Therapeutic Potential of d-MAPPS™ for Ocular Inflammatory Diseases and Regeneration of Injured Corneal and Retinal Tissue DOI Creative Commons

Carl Randall Harrell

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(21), С. 13528 - 13528

Опубликована: Ноя. 4, 2022

The invasion of microbial pathogens and/or sterile inflammation caused by physical/chemical injuries, increased ocular pressure, oxidative stress, and ischemia could lead to the generation detrimental immune responses in eyes, which result excessive tissue injury vision loss. bioavailability eye drops that are enriched with immunoregulatory trophic factors may concurrently suppress intraocular promote repair regeneration is generally low. We recently developed "derived- Multiple Allogeneic Proteins Paracrine Signaling regenerative biologics platform technology d-MAPPS™", a bioengineered biological product immunomodulatory can efficiently injured corneal retinal tissues. results obtained preclinical clinical studies showed d-MAPPS™ viability cells, inhibited production inflammatory cytokines alleviated inflammation, restored loss patients suffering from meibomian gland dysfunction dry disease. Herewith, we emphasized molecular mechanisms responsible for therapeutic efficacy presented main beneficial effects settings, indicating topical administration be considered new approach treatment diseases

Язык: Английский

Immunopathological mechanisms and clinical manifestations of ocular graft-versus-host disease following hematopoietic stem cell transplantation DOI
Rohan Bir Singh, Wonkyung Cho, Catherine Liu

и другие.

Bone Marrow Transplantation, Год журнала: 2024, Номер 59(8), С. 1049 - 1056

Опубликована: Май 31, 2024

Язык: Английский

Процитировано

9
Molecular nature of ocular surface barrier function, diseases that affect it, and its relevance for ocular drug delivery DOI Creative Commons
K. Paul Satinder,

Peri Sohnen,

Sudha Swamynathan

и другие.

The Ocular Surface, Год журнала: 2023, Номер 30, С. 3 - 13

Опубликована: Авг. 3, 2023

Язык: Английский

Процитировано

18
Cell therapy in the cornea: The emerging role of microenvironment DOI
Giuseppe Suanno, Vincenzo Giuseppe Genna, Eleonora Maurizi

и другие.

Progress in Retinal and Eye Research, Год журнала: 2024, Номер 102, С. 101275 - 101275

Опубликована: Май 24, 2024

Язык: Английский

Процитировано

7
Single cell analysis of short-term dry eye induced changes in cornea immune cell populations DOI Creative Commons
Jehan Alam,

Ebru Yaman,

Gerda Cristal Villalba Silva

и другие.

Frontiers in Medicine, Год журнала: 2024, Номер 11

Опубликована: Март 15, 2024

Background Dry eye causes corneal inflammation, epitheliopathy and sensorineural changes. This study evaluates the hypothesis that dry alters percentages transcriptional profiles of immune cell populations in cornea. Methods Desiccating stress (DS) induced was created by pharmacologic suppression tear secretion exposure to drafty low humidity environment. Expression profiling cells performed single-cell RNA sequencing (scRNA-seq). Cell differentiation trajectories fate were modeled through velocity analysis. Confocal microscopy used immunodetect cells. Irritation response topical neurostimulants assessed. Results Twelve based on their identified at baseline consist monocytes, resident (rMP) MMP12/13 high macrophages, dendritic (cDC2), neutrophils, mast cells, pre T/B innate (γDT, ILC2, NK) conventional T B lymphocytes. macrophages largest normal cornea comprising 18.6 18.2 percent, respectively. rMP increased 55.2% after 5 days DS. Significant changes expression 1,365 genes (adj p < 0.0001) noted with increases cytokines chemokines ( Tnf, Cxcl1, Ccl12, Il1rn ), inflammatory markers Vcam, Adam17, Junb TAM receptor Mertk decreases complement MHCII genes. A trajectory from monocytes terminal state found. Phagocytosis, C-type lectin signaling, NF-kappa signaling Toll-like among pathways enhanced activity these The percentage MRC1 + rMPs they observed basal epithelium adjacent epithelial nerve plexus. Concentration chemokine CXCL1 it heightened irritation/pain responses topically applied hypertonic saline. Conclusion These findings indicate DS recruits differentiate inflammation associated proximity nerves neurosensitizers suggests contribute eye.

Язык: Английский

Процитировано

6
Suppression of Neovascularization by Topical and Subconjunctival Bevacizumab After High-Risk Corneal Transplantation DOI Creative Commons
Thomas H. Dohlman, Rohan Bir Singh, Francisco Amparo

и другие.

Ophthalmology Science, Год журнала: 2024, Номер 4(4), С. 100492 - 100492

Опубликована: Фев. 13, 2024

PURPOSETo assess the effectiveness of topical and subconjunctival bevacizumab in suppressing vascularization graft host bed following high-risk corneal transplantation.DESIGNSecondary analysis prospective, randomized, double-blind, placebo-controlled multi-centric clinical trial.PARTICIPANTSThe study includes patients over 18 years old who underwent penetrating keratoplasty, which was defined as 1 or more quadrants excluding limbus.MethodsPatients were randomized to treatment control groups. The group received injection (2.5 mg/0.1 ml) on day procedure followed by (10 mg/ml) 4 times per for weeks. vehicle (0.9% sodium chloride) (carboxymethylcellulose 1%) a weeks.Main outcome measureVessel invasion area vessels beds.ResultsThis included 56 eyes transplantation, with equal numbers (control) group. mean age 61.2 ± 15.9 those treated 60.0 16.1 years. vessel at baseline comparable (16.72 3.19%) groups (15.48 3.12%, p=0.72). Similarly, areas also similar (35.60± 2.47%) (34.23 2.64%, p=0.9) baseline. reduction significantly higher (83.7%) period 52 weeks compared (61.5%, p<0.0001). In group, reduced 75.8% 46.5% post-procedure cases first (3.54 1.21%) repeat (3.80 0.40%) transplantation treatment. patients, (9.76 0.32%) (8.06 1.02%, p<0.0001) keratoplasty. week (11.70 3.38%) (11.64 1.74%) procedures, whereas (27.87 2.57%) (24.11 2.17%) keratoplasty patients.ConclusionsIn undergoing vascularized is efficacious reducing bed, thereby risk rejection beds.

Язык: Английский

Процитировано

5
Advances in the application of gelatin-based materials in anterior segment diseases DOI
Zhijian Zhang, Chuanfeng An, Chunxiao Yan

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142695 - 142695

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Morphological alterations in corneal nerves of patients with dry eye and associated biomarkers DOI

Yuichi Uchino,

Miki Uchino,

Miki Mizuno

и другие.

Experimental Eye Research, Год журнала: 2023, Номер 230, С. 109438 - 109438

Опубликована: Март 16, 2023

Язык: Английский

Процитировано

11
Imaging assessment of conjunctival goblet cells in dry eye disease DOI Creative Commons
Y Liu, Zhengyu Duan, Jin Yuan

и другие.

Clinical and Experimental Ophthalmology, Год журнала: 2024, Номер 52(5), С. 576 - 588

Опубликована: Март 30, 2024

Abstract Dry eye disease (DED) is a widespread, multifactorial, and chronic disorder of the ocular surface with disruption tear film homeostasis as its core trait. Conjunctival goblet cells (CGCs) are specialised secretory found in conjunctival epithelium that participate formation by secreting mucin. Changes both structure function CGCs hallmarks DED, imaging assessment important for diagnosis, classification, severity evaluation DED. Existing methods include biopsy, impression cytology vivo confocal microscopy, which can be used to assess morphology, distribution, density CGCs. Recently, moxifloxacin‐based fluorescence microscopy has emerged novel technique enables efficient, non‐invasive This article presents comprehensive overview their alterations context well current assessment. Additionally, potential directions visual discussed.

Язык: Английский

Процитировано

4
Allergic Conjunctivitis: Review of Current Types, Treatments, and Trends DOI Creative Commons

Fiza Tariq

Life, Год журнала: 2024, Номер 14(6), С. 650 - 650

Опубликована: Май 21, 2024

Allergic conjunctivitis is an allergen-induced immune response secondary to the binding of immunoglobulin-E (IgE) sensitized mast cells. Approximately 40% North Americans and 20% world’s population are impacted by some form allergy it continues increase in prevalence, especially among children. Specified IgE antibodies can be found almost all cases exposure seasonal or perennial allergens. Activation degranulation cells lead increased tear levels histamine, tryptase, leukotrienes, cytokines, prostaglandins. The release these factors initiates recruitment inflammatory conjunctival mucosa, which causes late-phase reaction. Signs symptoms ocular allergies include itching, tearing, chemosis, hyperemia, decreased productivity poor quality life. Many treatment options available improve symptoms, including, cell stabilizers, antihistamines, dual-acting agents, steroids, nonsteroidal anti-inflammatory drugs (NSAIDS), other off-label modalities. This review article provides overview different types allergic conjunctivitis, its pathology immunology, recommended methods treatment.

Язык: Английский

Процитировано

4
Mapping Resident Immune Cells in the Murine Ocular Surface and Lacrimal Gland by Flow Cytometry DOI Creative Commons

Baikai Ma,

Yifan Zhou,

Yuzhe Hu

и другие.

Ocular Immunology and Inflammation, Год журнала: 2023, Номер 31(4), С. 748 - 759

Опубликована: Март 3, 2023

Background The ocular surface and lacrimal gland have a frontline position in mucosal immunology. However, there been few updates to the immune cell atlas of these tissues recent years.Purpose To map cells murine gland.Methods Central peripheral corneas, conjunctiva, were dissociated into single suspensions, followed by flow cytometry. Discrepancy between central corneas was compared. In conjunctiva gland, myeloid clustered tSNE FlowSOM based on expression F4/80, Ly6C, Ly6G, MHC II. ILCs, type 1 cells, 3 analyzed.Results number about 16 folds that corneas. B accounted for 8.74% most tended out be monocytes, macrophages, classical dendritic (cDCs). ILC3 6.28% 3.63% ILCs respectively. Th1, Tc1, NK predominant cells. γδ T17 outnumbered Th17 among T cells.Conclusion resident reported first time. Additionally, we proposed strategy clustering better understand their heterogeneity FlowSOM. Furthermore, identified Compositions summarized. Our study provides fundamental reference novel insights homeostasis diseases.

Язык: Английский

Процитировано

9