Pharmacology Research & Perspectives,
Год журнала:
2024,
Номер
13(1)
Опубликована: Дек. 26, 2024
ABSTRACT
The
lack
of
effective
treatments
for
dry
age‐related
macular
degeneration
(AMD)
is
in
part
due
to
a
preclinical
animal
model
that
recapitulates
features
the
clinical
state
including
retinal
pigment
epithelium
(RPE)
degeneration,
also
known
as
geographic
atrophy
(GA).
A
nonhuman
primate
GA
was
developed
and
its
responsiveness
an
approved
treatment,
avacincaptad
pegol
(ACP),
complement
C5
inhibitor,
evaluated.
Intravitreal
(ivt)
administration
sodium
iodate
(SI)
into
one
eye
male
Macaca
fascicularis
leads
areas
(mm
2
)
hyper‐
or
hypo‐autofluorescence.
Qualitative
changes
structure
over
time
were
observed
with
spectral
domain
optical
coherence
tomography
(OCT).
Six
days
after
SI
administration,
prior
mean
(±
SEM)
all
eyes
8.2
±
1.8
mm
.
Following
randomization
treatment
groups,
either
vehicle
ACP
ivt
injected
continued
every
4
weeks,
total
four
treatments.
Sixteen
weeks
area
vehicle‐treated
18.9
6.6
,
whereas
ACP‐treated
11.4
4.0
reduction
by
about
36%.
Increased,
followed
decreased,
overall
thickness
OCT
following
administration.
Treatment
did
not
change
alter
thinning.
Geographic
atrophy‐like
lesions
expand
are
current
macaque
could
be
utilized
further
explore
mechanism
AMD
develop
more
novel
therapeutics.
Experimental Gerontology,
Год журнала:
2024,
Номер
196, С. 112556 - 112556
Опубликована: Авг. 27, 2024
The
aim
of
this
study
was
to
evaluate
the
potential
protective
effect
Qiju
Granule
in
a
rat
model
age-related
macular
degeneration
(AMD)
and
investigate
underlying
mechanisms
involved.
European journal of medical research,
Год журнала:
2024,
Номер
29(1)
Опубликована: Дек. 23, 2024
Age-related
macular
degeneration
(AMD),
is
a
neurodegenerative
ocular
disease.
This
study
investigated
the
role
of
ferroptosis-related
genes
and
their
interaction
with
immune
cell
infiltration
in
AMD.
We
screened
differential
expression
(DEGs)
AMD
from
data
sets
Gene
Expression
Omnibus.
identified
differentially
expressed
(ferroDEGs)
by
intersecting
DEGs
genes.
Protein–protein
interactions
network
Cytoscape
were
used
for
screening
hub
Next,
we
analyzed
using
CIBERSORT
examined
crosstalk
between
ferroDEGs
infiltration.
Hub
each
cluster
proportions
different
clusters
normal
samples
single-cell
data.
The
ferroDEG
expressions
verified
mouse
models
RT–qPCR,
western
blot,
immunofluorescence
assay.
roles
ANXA1
ferroptosis
its
microglia
investigated.
that
include
six
(ANXA1,
DKK1,
CD44,
VIM,
TGFB2,
DUSP1).
Functional
analysis
those
was
found
to
be
correlated
leukocyte
migration
chemotaxis,
macrophage
migration,
gliogenesis.
high-risk
group
exhibited
elevated
levels
CD8+
T
cells,
activated
NK
M2
macrophages.
Single-cell
sequencing
revealed
high
degree
heterogeneity
monocytes
proportion
area
higher
samples.
Moreover,
observed
mRNA
protein
(P
<
0.01),
while
knockdown
reduced
GPX4
model.
Finally,
validated
increased
colocalization
assays.
offers
insights
into
pathogenesis
identifies
as
potential
target
related
protecting
response
future
research.
Pharmacology Research & Perspectives,
Год журнала:
2024,
Номер
13(1)
Опубликована: Дек. 26, 2024
ABSTRACT
The
lack
of
effective
treatments
for
dry
age‐related
macular
degeneration
(AMD)
is
in
part
due
to
a
preclinical
animal
model
that
recapitulates
features
the
clinical
state
including
retinal
pigment
epithelium
(RPE)
degeneration,
also
known
as
geographic
atrophy
(GA).
A
nonhuman
primate
GA
was
developed
and
its
responsiveness
an
approved
treatment,
avacincaptad
pegol
(ACP),
complement
C5
inhibitor,
evaluated.
Intravitreal
(ivt)
administration
sodium
iodate
(SI)
into
one
eye
male
Macaca
fascicularis
leads
areas
(mm
2
)
hyper‐
or
hypo‐autofluorescence.
Qualitative
changes
structure
over
time
were
observed
with
spectral
domain
optical
coherence
tomography
(OCT).
Six
days
after
SI
administration,
prior
mean
(±
SEM)
all
eyes
8.2
±
1.8
mm
.
Following
randomization
treatment
groups,
either
vehicle
ACP
ivt
injected
continued
every
4
weeks,
total
four
treatments.
Sixteen
weeks
area
vehicle‐treated
18.9
6.6
,
whereas
ACP‐treated
11.4
4.0
reduction
by
about
36%.
Increased,
followed
decreased,
overall
thickness
OCT
following
administration.
Treatment
did
not
change
alter
thinning.
Geographic
atrophy‐like
lesions
expand
are
current
macaque
could
be
utilized
further
explore
mechanism
AMD
develop
more
novel
therapeutics.
