Hyperosmotic stress-induced NLRP3 inflammasome activation via the mechanosensitive PIEZO1 channel in dry eye corneal epithelium

The Ocular Surface, Год журнала: 2025, Номер 36, С. 106 - 118

Опубликована: Янв. 18, 2025

Язык: Английский

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Exosomes in Ocular Health: Recent Insights into Pathology, Diagnostic Applications and Therapeutic Functions

Biomedicines, Год журнала: 2025, Номер 13(1), С. 233 - 233

Опубликована: Янв. 19, 2025

Exosomes are extracellular vesicles ranging from 30 to 150 nm in diameter that contain proteins, nucleic acids and other molecules. Produced by virtually all cell types, they travel throughout the body until reach their target, where can trigger a wide variety of effects transferring molecular cargo recipient cells. In context ocular physiology, exosomes play very important role embryological development, regulation homeostasis immune system, which is crucial for normal vision. Consequently, pathological situations, also undergo modifications terms quantity, composition content, depending on etiology disease. However, mechanisms contribute pathology has not yet been studied depth, many questions remain unanswered. This review aims summarize most recent knowledge function system healthy individuals during processes degenerative, infectious, neurodegenerative, vascular inflammatory nature, such as keratoconus, keratitis, glaucoma, diabetic retinopathy uveitis. Furthermore, given unique characteristics, potential diagnostic biomarkers or therapeutic agents application clinical ophthalmology explored, along with main limitations researchers face today field.

Язык: Английский

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Oridonin Preserves Retinal Pigmented Epithelial Cell Tight Junctions and Ameliorates Choroidal Neovascularization

Investigative Ophthalmology & Visual Science, Год журнала: 2025, Номер 66(2), С. 56 - 56

Опубликована: Фев. 21, 2025

To investigate the role and mechanism of oridonin (ORI), a bioactive diterpenoid extracted from Chinese herbal medicine Rabdosia rubescens, on integrity outer blood-retinal barrier (oBRB) during choroidal neovascularization (CNV). ARPE-19 cells were exposed to hypoxia treated with ORI. The expression ZO-1 occludin in axis TGFβR/SUV39H1/KLF11 was detected by WB, chromatin immunoprecipitation, luciferin report activity assay, immunofluorescence assay (IF), effect ORI properties studied. A laser-induced mouse CNV model constructed, administrated oral gavage. IF choroid flat mounts done confirm BRB integrity. Indocyanine green angiography retina-RPE-choroid performed determine formation retinal function. Hematoxylin eosin staining TUNEL carried out appraise ocular systemic cytotoxicity caused protected hypoxia-induced destruction promoted axis, maintaining hypoxia. improved mice mitigated without any or (n = 4-5 each group). ameliorates subsequent via regulating pathway RPE cells.

Язык: Английский

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Oroxylin A alleviates pyroptosis and apoptosis in human corneal epithelial cells under hyperosmotic stress by activating the SIRT3-SOD2/HIF-1α pathway

Experimental Eye Research, Год журнала: 2025, Номер 255, С. 110345 - 110345

Опубликована: Март 15, 2025

Язык: Английский

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TRPV1-targeted ion-responsive hydrogel against pyroptosis of dry eye disease

Chemical Engineering Journal, Год журнала: 2024, Номер unknown, С. 156155 - 156155

Опубликована: Сен. 1, 2024

Язык: Английский

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Oridonin attenuates diabetic retinopathy progression by suppressing NLRP3 inflammasome pathway

Molecular and Cellular Endocrinology, Год журнала: 2024, Номер unknown, С. 112419 - 112419

Опубликована: Ноя. 1, 2024

Язык: Английский

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Corneal Mucin‐Targeting Liposome Nanoplatforms Enable Effective Treatment of Dry Eye Diseases by Integrated Regulation of Ferroptosis and Inflammation

Advanced Science, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 28, 2024

Abstract The incidence of dry eye disease (DED) has been increasing annually worldwide, creating an urgent need for new therapies. Due to the multifactorial mechanism underlying DED, traditional medications focused on decreasing ocular surface inflammation have unable address all harmful factors and fail achieve a complete clinical cure. Ferroptosis, form programmed cell death characterized by lipid peroxidation, become pivotal contributor oxidative stress‐driven pathology. Therefore, therapeutic targeting ferroptosis may be attractive option management. Herein, sialic acid‐targeting peptide‐modified liposome loaded with Cyclosporine A (CsA), typical anti‐inflammatory drug, Ferrostatin‐1 (Fer‐1), selective inhibitor, is developed termed as CF@SNPs, combing sustaining DED treatment. This multifunctional liposomal encapsulation demonstrates excellent aqueous solubility; moreover, peptide prolongs retention, further enhancing efficacy. CF@SNPs treatment comprehensively alleviates symptoms, including improving corneal defects, augmenting goblet count, restoring tear secretion. Specifically, attenuate pathology suppressing p53‐SLC7A11‐GSH‐dependent TNF‐α‐associated inflammatory cascades, accompanied favorable biocompatibility in vivo. These results underscore promising potential this superior nano‐formulation pharmacotherapy.

Язык: Английский

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Run-Mu-Ling Granules Mitigate Ocular Surface Inflammatory Injury Associated with Dry Eye by Suppressing the NLRP3/GSDMD-Mediated Pyroptosis Pathway

Journal of Inflammation Research, Год журнала: 2024, Номер Volume 17, С. 10769 - 10784

Опубликована: Дек. 1, 2024

Run-Mu-Ling granules (RMLG), a traditional Chinese medicinal formula, are used to treat dry eye (DE); however, the underlying mechanism remains poorly understood. This study aimed elucidate potential molecular mechanisms by which RMLG alleviates ocular surface inflammation in DE.

Язык: Английский

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Cell Death Pathways in Dry Eye Disease: Insights into Ocular Surface Inflammation

The Ocular Surface, Год журнала: 2024, Номер 34, С. 535 - 544

Опубликована: Окт. 1, 2024

Язык: Английский

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