Sauchinone Ameliorates Senescence Through Reducing Mitochondrial ROS Production

Antioxidants, Год журнала: 2025, Номер 14(3), С. 259 - 259

Опубликована: Фев. 24, 2025

One of the major causes senescence is oxidative stress caused by ROS, which mainly generated from dysfunctional mitochondria. Strategies to limit mitochondrial ROS production are considered important for reversing senescence, but effective approaches reduce them have not yet been developed. In this study, we screened secondary metabolites that plants produce under and discovered sauchinone as a potential candidate. Sauchinone induced function recovery, enabling efficient electron transport within chain (ETC). This led decrease in production, byproduct inefficient transport. The reduction rejuvenated senescence-associated phenotypes. To understand underlying mechanism rejuvenates carried out RNA sequencing found VAMP8 key gene. was downregulated sauchinone. Knockdown decreased levels subsequently function, similar effect Taken together, these studies revealed novel reduces regulating expression. Our results open new avenue aging research control production.

Язык: Английский

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Molecular Mechanisms of the Anti-Inflammatory Effects of Epigallocatechin 3-Gallate (EGCG) in LPS-Activated BV-2 Microglia Cells

Brain Sciences, Год журнала: 2023, Номер 13(4), С. 632 - 632

Опубликована: Апрель 7, 2023

Chronic neuroinflammation is associated with many neurodegenerative diseases, such as Alzheimer's. Microglia are the brain's primary immune cells, and when activated, they release various proinflammatory cytokines. Several natural compounds anti-inflammatory antioxidant properties, epigallocatechin 3-gallate (EGCG), may provide a promising strategy for inflammation-related diseases involving activated microglia cells. The objective of current study was to examine molecular targets underlying effects EGCG in BV-2 cells were grown, stimulated, treated EGCG. Cytotoxicity nitric oxide (NO) production evaluated. Immunoassay, PCR array, WES™ Technology utilized evaluate inflammatory, neuroprotective modulators well signaling pathways involved mechanistic action neuroinflammation. Our findings showed that significantly inhibited mediator NO LPS-stimulated In addition, ELISA analysis revealed decreases cytokine IL-6 while it increases TNF-α. array downregulated MIF, CCL-2, CSF2. It also upregulated IL-3, IL-11, TNFS10. Furthermore, inflammatory mRNA expression mTOR, NF-κB2, STAT1, Akt3, CCL5, SMAD3 upregulating Ins2, Pld2, A20/TNFAIP3, GAB1. Additionally, reduced relative protein Akt3. These suggest be used its prevent diseases.

Язык: Английский

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Senescence Rejuvenation through Reduction in Mitochondrial Reactive Oxygen Species Generation by Polygonum cuspidatum Extract: In Vitro Evidence

Antioxidants, Год журнала: 2024, Номер 13(9), С. 1110 - 1110

Опубликована: Сен. 14, 2024

Oxidative stress caused by reactive oxygen species (ROS) is one of the major causes senescence. Strategies to reduce ROS are known be important factors in reversing senescence, but effective strategies have not been found. In this study, we screened substances commonly used as cosmetic additives find with antioxidant effects. Polygonum cuspidatum (P. cuspidatum) extract significantly reduced levels senescent cells. A novel mechanism was discovered which P. ROS, a byproduct inefficient oxidative phosphorylation (OXPHOS), increasing OXPHOS efficiency. The reduction restored senescence-associated phenotypes and enhanced skin protection. Then, identified polydatin active ingredient that exhibited Polydatin, contains stilbenoid polyphenols act singlet scavengers through redox reactions, increased efficiency subsequently phenotypes. summary, our data confirmed effects on senescence rejuvenation protection reduction. This finding may treatment clinical fields.

Язык: Английский

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Identification of senescence rejuvenation mechanism of Magnolia officinalis extract including honokiol as a core ingredient

Aging, Год журнала: 2025, Номер unknown

Опубликована: Фев. 21, 2025

Aging | doi:10.18632/aging.206207. Yun Haeng Lee, Eun Young Jeong, Ye Hyang Kim, Ji Ho Park, Jee Hee Yoon, Yoo Jin So Hun Yeon Kyung Nam, Yoon Cha, Seong Youngjoo Byun, Song Seok Shin, Joon Tae Park

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Epigallocatechin Gallate in Camellia sinensis Ameliorates Skin Aging by Reducing Mitochondrial ROS Production

Pharmaceuticals, Год журнала: 2025, Номер 18(5), С. 612 - 612

Опубликована: Апрель 23, 2025

Background: Reactive oxygen species (ROS) generated by mitochondrial dysfunction damage cellular organelles and contribute to skin aging. Therefore, strategies reduce ROS production are considered important for alleviating aging, but no effective methods have been identified. Methods: In this study, we evaluated substances utilized as cosmetic ingredients discovered Camellia sinensis (C. sinensis) a substance that reduces levels. Results: C. extracts were found act senolytics selectively kill senescent fibroblasts containing dysfunctional mitochondria. addition, facilitated efficient electron transport in the chain (ETC) increasing efficiency of oxidative phosphorylation (OXPHOS), thereby reducing production, byproduct inefficient ETC. This novel mechanism led restoration aging barrier. Furthermore, epigallocatechin gallate (EGCG) was identified an active ingredient plays key role extract-mediated recovery. Indeed, similar extracts, EGCG reduced improved artificial model. Conclusions: Our data uncovered which extract reverses via selective cell death/increased OXPHOS efficiency. results suggest or may be used therapeutic agent reverse clinical applications.

Язык: Английский

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Synergistic ROS Reduction Through the Co-Inhibition of BRAF and p38 MAPK Ameliorates Senescence

Antioxidants, Год журнала: 2024, Номер 13(12), С. 1465 - 1465

Опубликована: Ноя. 28, 2024

Reactive oxygen species (ROS)-mediated damage to macromolecules and cellular organelles is one of the major causes senescence. Therapeutic strategies that lower ROS levels have been proposed as important treatments for senescence, but effective mechanisms reducing not discovered. Here, we aimed find a combination has synergistic effect on reduction using senomorphics known reduce ROS. Combination treatment with BRAF inhibitor SB590885 p38 MAPK SB203580 showed compared either drug alone. The through this led restored mitochondrial function ameliorated senescence-associated phenotypes. To elucidate underlying mechanism by which two drugs reverses performed RNA sequencing identified metallothionein 2A (MT2A) key gene. MT2A was upregulated in response therapy, overexpression decrease subsequent recovery phenotypes, similar effects therapy. Taken together, found reduction, contributed phenotypes gene regulation. This study opens up new avenue aging research demonstrating therapy existing can enhance ability reverse senescence reversal be achieved regulation regulated

Язык: Английский

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Antioxidant and anti-ageing effects of oleuropein aglycone in canine skeletal muscle cells

Tissue and Cell, Год журнала: 2024, Номер 88, С. 102369 - 102369

Опубликована: Март 29, 2024

Reactive oxygen species (ROS) are normally produced in skeletal muscle. However, an imbalance their regulatory systems can lead to accumulation and ultimately oxidative stress, which is one of the causes ageing process. Companion dogs share same environment lifestyle as humans, making them excellent comparative model for study ageing, well they constitute a growing market bioactive molecules that improve quality life pets. The anti-ageing properties oleuropein aglycone (OLE), compound from olive leaves known its antioxidant properties, were investigated Myok9 canine muscle cell model. After incubation with OLE, senescence was induced cellular by hydrogen peroxide (H2O2). Analyses performed on cells after seven days differentiation. stress H2O2 treatment differentiated led significant increase ROS formation, reduced OLE pretreatment alone or combination about 34% 32%, respectively. Cells treated showed 48% area senescent stained SA-β-gal, while significantly coloured 52%. H2O2, activity, possibly through autophagy activation, indicated expression autophagic markers.

Язык: Английский

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Therapy-induced senescence in breast cancer: an overview

Exploration of Targeted Anti-tumor Therapy, Год журнала: 2024, Номер 5(4), С. 902 - 920

Опубликована: Июль 25, 2024

Outcomes for women with breast cancer have improved dramatically in recent decades. However, many patients present intrinsic drug resistance and others are initially sensitive to anti-cancer drugs but acquire during the course of their treatment, leading recurrence and/or metastasis. Drug therapy-induced senescence (TIS) is a form characterised by induction cell cycle arrest emergence senescence-associated secretory phenotype (SASP) that can develop response chemo- targeted- therapies. A wide range anticancer interventions lead SASP induction, inducing genotoxic stress, hyperactivation signalling pathways or oxidative stress. TIS be anti-tumorigenic short-term, pro-tumorigenic long-term creating pro-inflammatory immunosuppressive microenvironment. Moreover, promote angiogenesis epithelial-mesenchymal transition neighbouring cells. In this review, we will describe characteristics detail changes accompany its induction. We also discuss strategies targeting senescent cells order prevent delay tumour recurrence.

Язык: Английский

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What matters in aging is signaling for responsiveness

Pharmacology & Therapeutics, Год журнала: 2023, Номер 252, С. 108560 - 108560

Опубликована: Ноя. 10, 2023

Язык: Английский

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