The
enzymatic
modification
of
proteins
using
protein
hydrolase
preparations
to
prepare
bioactive
peptides
has
been
widely
used
in
the
food
industry.
In
this
paper,
dynamic
changes
four
exogenous
enzymes
and
reaction
time
on
process
prepared
sea
cucumber
were
characterized
at
level
macromolecules
peptide
small
molecules
further
reveal
their
effects
reaction.
239
precursor
with
6734
sequences
(3-25
amino
acid
residues)
identified
from
7
group
digestion
products
(Holothuria
leucospilota)
peptidome
analysis.
620
shared
detected
groups
different
hydrolysis
times,
while
177
proteases.
relative
abundance
distribution
non-shared
varied
methods,
revealing
dynamics
HL
proteins.
structural
characteristics
physicochemical
properties
each
differentially
analyzed
combination
bioanalytical
methods.
Monitoring
a
microscopic
perspective
can
help
production
quality
control
functional
cucumbers.
LWT,
Год журнала:
2023,
Номер
191, С. 115648 - 115648
Опубликована: Дек. 14, 2023
Endogenous
peptides
and
proteins
are
important
nutrients
in
milk
participants
various
biological
processes.
In
the
present
study,
we
compared
endogenous
parent
bovine
from
different
lactation
stages
using
4D
label-free
technique.
were
identified
by
database
search
or
de
novo
analysis.
Through
searches,
matched
10186
milk,
which
derived
1147
proteins.
By
analysis,
a
total
of
1991
peptides.
Among
these
peptides,
4852
differentially
expressed
(DEEPs)
between
colostrum
(BC)
mature
(BM)
found
with
bioactivities,
including
ACE-inhibitory,
immunomodulatory
anticancer
activities.
Gene
Ontology
(GO)
annotation
showed
that
BC
BM
mainly
structural
molecule
activity
category
within
molecular
function
subgroup.
The
primary
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
pathway
was
related
to
ribosomes.
These
results
reveal
changes
functions
activities
during
provide
novel
insight
into
developing
infant
formula
powder
based
on
sources.
Food & Function,
Год журнала:
2023,
Номер
14(18), С. 8261 - 8275
Опубликована: Янв. 1, 2023
Donkey
colostrum,
due
to
its
abundance
of
active
ingredients,
including
lysozyme,
proteins,
and
peptides,
is
essential
for
the
growth
immune
defence
newborns.
However,
research
on
endogenous
peptides
in
donkey
colostrum
inadequate.
This
study
analysed
profiles
their
potential
bioactivity,
enzymes
that
generated
these
using
two
different
strategies.
A
total
6202
were
characterised
through
a
database
search,
while
an
additional
2997
identified
de
novo.
Among
1142
proteins
identified,
trypsin
plasmin
demonstrated
highest
bioactivities.
Furthermore,
bioinformatics-based
screening
antioxidant
angiotensin
I-converting
enzyme
inhibitory
dipeptidyl
peptidase
IV
as
three
most
peptides.
Gene
Ontology
(GO)
annotation
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
pathway
analyses
conducted.
These
findings
enhance
our
knowledge
provide
crucial
information
regarding
nutritional
factors
future
development
functional
foods
derived
from
sources.
Dairy,
Год журнала:
2023,
Номер
4(1), С. 150 - 166
Опубликована: Фев. 3, 2023
The
destabilization
of
UHT
milk
during
its
shelf
life
can
be
promoted
by
the
residual
proteolytic
activity
attributed
to
protease
AprX
from
Pseudomonas.
To
better
understand
hydrolysis
patterns
AprX,
and
evaluate
feasibility
using
low-temperature
inactivation
(LTI)
for
release
peptides
through
on
proteins
was
examined
an
LC-MS/MS-based
peptidomic
analysis.
Milk
samples
were
either
directly
incubated
hydrolyzed
or
preheated
under
LTI
conditions
(60
°C
15
min)
then
incubated.
Peptides
parent
(the
which
originated)
identified
quantified.
mapped
cleavage
frequency
amino
acids
in
P1/P1′
positions
analyzed,
after
influence
potential
bitterness
formed
determined.
Our
results
showed
that
a
total
2488
48
proteins,
with
most
abundant
originating
κ-casein
β-casein.
may
also
non-specifically
hydrolyze
other
milk.
Except
decreasing
skim
milk,
did
not
significantly
reduce
AprX-induced
proteins.
Therefore,
feasible
production.
Molecular Nutrition & Food Research,
Год журнала:
2024,
Номер
68(8)
Опубликована: Апрель 1, 2024
Scope
In
cases
where
breast
milk
is
unavailable
or
inadequate,
hydrolyzed
infant
formula
recommended
as
the
primary
alternative.
The
aim
of
this
study
to
assess
and
compare
allergenicity
two
partially
whey‐based
formulas
(PHF‐Ws)
using
serum
samples
from
patients
with
cow's
allergy
(CMA).
Methods
results
LC‐MS/MS
technology
used
investigate
peptide
distribution
in
both
samples.
immunoreactivity
PHF‐Ws
27
50
Chinese
infants
(02
years)
CMA
analyzed.
demonstrate
that
even
a
similar
degree
hydrolysis
(DH),
protein
sources,
peptides
molecular
weights
<5
kDa,
differences
number
residual
allergenic
epitopes
segments
can
lead
varying
immune
responses.
Conclusion
have
notably
high
intolerance
rates,
exceeding
10%
among
CMA.
Therefore,
suggesting
may
not
be
suitable
for
children
China.
Journal of Dairy Science,
Год журнала:
2024,
Номер
107(10), С. 7594 - 7608
Опубликована: Июнь 7, 2024
Mare
milk
has
a
unique
protein
composition
that
makes
it
preferred
option
for
adult
and
infant
nutrition.
Several
functional
properties
have
been
attributed
to
this
milk,
but
with
little
current
evidence.
In
fact,
knowledge
on
mare
is
still
limited.
particular,
studies
addressing
the
performance
of
proteins
during
human
gastrointestinal
digestion
are
scarce,
which
limits
understanding
nutritional
quality
functionality.
For
reason,
present
study
describes
digestibility
release
peptides
as
affected
by
management
lactation
stage,
factors
known
affect
composition.
samples
from
3
different
farms
collected
6
mo
(n
=
54)
were
subjected
static
in
vitro
model
measure
peptide
digestibility.
study,
detailed
description
individual
amino
acid
behavior
process
was
given.
first
time,
2
equine
β-lactoglobulin
isoforms
(I
II)
described
individually.
addition,
found
stage
system
can
significantly
milk.
Presumably,
differences
influence
structure
enzyme
accessibility,
might
an
impact
behavior.
Although
no
specific
bioactive
identified,
several
precursors
previously
found.
These
findings
could
support
idea
food
added
value.
The
enzymatic
modification
of
proteins
using
protein
hydrolase
preparations
to
prepare
bioactive
peptides
has
been
widely
used
in
the
food
industry.
In
this
paper,
dynamic
changes
four
exogenous
enzymes
and
reaction
time
on
process
prepared
sea
cucumber
were
characterized
at
level
macromolecules
peptide
small
molecules
further
reveal
their
effects
reaction.
239
precursor
with
6734
sequences
(3-25
amino
acid
residues)
identified
from
7
group
digestion
products
(Holothuria
leucospilota)
peptidome
analysis.
620
shared
detected
groups
different
hydrolysis
times,
while
177
proteases.
relative
abundance
distribution
non-shared
varied
methods,
revealing
dynamics
HL
proteins.
structural
characteristics
physicochemical
properties
each
differentially
analyzed
combination
bioanalytical
methods.
Monitoring
a
microscopic
perspective
can
help
production
quality
control
functional
cucumbers.
