Encapsulation of Immobilized β-Glucosidase with Calcium Metal–Organic Frameworks for Enhanced Stability in Hydrolysis of Cellobiose DOI
Lili Xu,

Huanruo Liu,

Xiaohan Wang

и другие.

Langmuir, Год журнала: 2024, Номер 40(35), С. 18727 - 18735

Опубликована: Авг. 19, 2024

β-Glucosidase (β-G) holds promising applications in various fields, such as biomass energy, food, pharmaceuticals, and environmental protection, yet its industrial application is still limited by issues of stability recycling. Herein, we first immobilized β-G onto the surface magnetic chitosan nanoparticles (MCS/β-G) through adsorption methods. Subsequently, utilizing metal–organic framework (MOF), CaBDC, which possesses good under acidic conditions, encapsulated MCS/β-G. The resulting biocatalyst (MCS/β-G@CaBDC) exhibited excellent activity recyclability. MCS/β-G@CaBDC can convert 91.5% cellobiose to glucose 60 min maintained 81.9% after 10 cycles. apparent Km value was 0.148 mM, lower than free (0.166 mM) MCS/β-G (0.173 mM). CaBDC layer increased mass transfer resistance reaction but also triggered structural rearrangement during encapsulation process. This resulted β-sheet content rising 68.4%, which, turn, contributed enhancing rigidity β-G. Moreover, saturated strength this could reach 37.3 emu/g, facilitating recovery. prepared study exhibits prospects, immobilization method provide valuable insights into field enzyme immobilization.

Язык: Английский

High-internal-phase emulsions stabilized by egg high-density lipoprotein and phosvitin: Improvement of properties free from polysaccharides DOI

Shuze Ren,

Mahmoud Abou-Elsoud, Xi Huang

и другие.

Food Hydrocolloids, Год журнала: 2024, Номер 158, С. 110487 - 110487

Опубликована: Авг. 1, 2024

Язык: Английский

Процитировано

6
Encapsulation of Immobilized β-Glucosidase with Calcium Metal–Organic Frameworks for Enhanced Stability in Hydrolysis of Cellobiose DOI
Lili Xu,

Huanruo Liu,

Xiaohan Wang

и другие.

Langmuir, Год журнала: 2024, Номер 40(35), С. 18727 - 18735

Опубликована: Авг. 19, 2024

β-Glucosidase (β-G) holds promising applications in various fields, such as biomass energy, food, pharmaceuticals, and environmental protection, yet its industrial application is still limited by issues of stability recycling. Herein, we first immobilized β-G onto the surface magnetic chitosan nanoparticles (MCS/β-G) through adsorption methods. Subsequently, utilizing metal–organic framework (MOF), CaBDC, which possesses good under acidic conditions, encapsulated MCS/β-G. The resulting biocatalyst (MCS/β-G@CaBDC) exhibited excellent activity recyclability. MCS/β-G@CaBDC can convert 91.5% cellobiose to glucose 60 min maintained 81.9% after 10 cycles. apparent Km value was 0.148 mM, lower than free (0.166 mM) MCS/β-G (0.173 mM). CaBDC layer increased mass transfer resistance reaction but also triggered structural rearrangement during encapsulation process. This resulted β-sheet content rising 68.4%, which, turn, contributed enhancing rigidity β-G. Moreover, saturated strength this could reach 37.3 emu/g, facilitating recovery. prepared study exhibits prospects, immobilization method provide valuable insights into field enzyme immobilization.

Язык: Английский

Процитировано

1