Microwave‐assisted synthesis of 4,4‐dimethylsterol‐conjugated linoleic acid ester and its lipid‐lowering benefits evaluation
Journal of Food Science,
Год журнала:
2025,
Номер
90(4)
Опубликована: Апрель 1, 2025
Abstract
4,4‐Dimethylsterol
(RST),
rich
in
rice
bran
oil,
is
often
considered
to
have
good
lipid‐lowering
properties,
and
conjugated
linoleic
acid
(CLA),
a
special
fatty
acid,
also
similar
but
the
low
solubility
poor
oxidation
stability
limits
their
application
food
industry.
Typically,
sterol
esters
better
after
esterification.
However,
it
difficult
esterify
4,4‐dimethylsterol
for
its
additional
methyl
groups.
Therefore,
microwave‐assisted
esterification
of
with
CLA
was
developed
this
study,
function
4,4‐dimethylsterol–CLA
(RSE)
evaluated
high‐fat
diet
(HFD)‐induced
mice
model.
The
results
showed
that
when
reaction
condition
molar
ratio
RST
1:3
catalyzed
5%
(mol%)
p‐toluene
sulfonic
25%
dodecyl
benzene
maintained
at
microwave
reactor
125
min
100°C,
highest
rate
93.63%
acquired.
Furthermore,
HFD‐induced
experiments
demonstrated
RSE
could
significantly
reduce
body
weight,
as
well
serum
liver
triglyceride
(TG)
levels,
while
exert
hepatoprotective
properties
compared
RST.
Additionally,
found
affect
appetite
regulate
expression
lipid
metabolism–related
genes
mice.
These
findings
suggest
holds
potential
field
functional
foods.
Язык: Английский
Enzymatic Synthesis of Phytosterol Esters Using Recombinant E. Coli Whole Cell Expressing Lipase from Burkholderia Sp
Опубликована: Янв. 1, 2025
Язык: Английский
Dual role of ionic liquid modified β-Cyclodextrin in dynamic conformational regulation and substrate enrichment for improved lipase immobilization
Carbohydrate Polymers,
Год журнала:
2025,
Номер
unknown, С. 123786 - 123786
Опубликована: Май 1, 2025
Язык: Английский
Protective effect of ginseng seed oil nanoemulsion against human coronavirus OC43 through modulation of ABCG transporters
Journal of Functional Foods,
Год журнала:
2024,
Номер
117, С. 106240 - 106240
Опубликована: Май 20, 2024
Recent
studies
have
highlighted
the
potential
of
unsaturated
fatty
acids
in
natural
oils
to
influence
virus
replication.
This
study
extracted
ginseng
seed
oil
(GSO)
and
evaluated
its
physiochemical
parameters,
lipid
class,
oxidation
stability.
GSO
was
then
synthesized
into
an
oil/water
nanoemulsion
(NE)
through
optimized
formulation,
time,
temperature,
storage
conditions.
GSO-NE
showed
no
cytotoxicity
up
5
µg/mL
but
effectively
inhibited
HCoV-OC43
replication
suppressed
proinflammatory
biomarkers
infected
cells.
RNA
sequencing
revealed
significant
alterations
gene
expression
profile
by
treatment,
particularly
implicating
ABC
transporters
signaling
pathway.
Notably,
downregulated
ABCG
subfamily
membrane
proteins,
crucial
for
regulation
virus-infected
The
downregulation
these
genes
(ABCG1,
ABCG2,
ABCG4,
ABCG5/8)
reversed
GSO-NE,
as
demonstrated
qRT-PCR.
Furthermore,
ABCG1
ABCG5/8
confirmed
using
immunoblotting
techniques.
These
findings
suggest
a
promising
avenue
future
research
or
therapeutic
applications.
Язык: Английский
Intensification of the process of ergosterol accumulation by yeast Saccharomyces cerevisiae by changing the composition of the culture medium and using ultrasound
BIO Web of Conferences,
Год журнала:
2024,
Номер
139, С. 08003 - 08003
Опубликована: Янв. 1, 2024
The
chemical
synthesis
of
vitamin
D2,
an
important
nutrient
for
living
organisms,
is
complex
due
to
the
asymmetric
structure
molecule.
This
opens
up
significant
prospects
development
biotechnological
approaches
cyanocobalamin
synthesis,
which
forms
basis
this
work.
study
considers
possibility
using
media
different
compositions,
as
well
ultrasound
exposure
intensify
biosynthesis
ergosterol
by
yeast
Saccharomyces
cerevisiae
a
precursor
D2.
standard
YEPD
medium
considered
medium,
low-carb
and
sodium
nitrate
dilution.
modes
ultrasonic
varied
in
power
processing
time,
9
variants
were
evaluated.
Together,
intensification
process
cells
can
ensure
economic
viability,
stability,
expediency
its
production
on
industrial
scale.
research
results
have
shown
proposed
approaches.
Thus,
introduction
into
allowed
increase
synthesized
biomass
18.2%
compared
with
medium.
Optimization
treatment
has
achieving
content
11.82%
per
dry
matter.
Язык: Английский