Functional Analysis of Durum Wheat GASA1 Protein as a Biotechnological Alternative Against Plant Fungal Pathogens and a Positive Regulator of Biotic Stress Defense

Plants, Год журнала: 2025, Номер 14(1), С. 112 - 112

Опубликована: Янв. 2, 2025

Plants are frequently challenged by a variety of microorganisms. To protect themselves against harmful invaders, they have evolved highly effective defense mechanisms, including the synthesis numerous types antimicrobial peptides (AMPs). Snakins such compounds, encoded GASA (Gibberellic Acid-Stimulated Arabidopsis) gene family, and involved in response to biotic abiotic stress. Here, we examined function newly identified TdGASA1 its protein Triticum durum subjected different stress-related simulants, as mechanical injury, methyl jasmonate (MeJA), indole-3-acetic acid (IAA), salicylic (SA), hydrogen peroxide (H2O2), well infection with pathogenic fungi Fusarium graminearum Aspergillus niger. We found that wheat, transcripts were markedly increased these stress simulants. Isolated purified exhibited significant antifungal activity growth inhibition test conducted on eight species solid liquid media. Transgenic Arabidopsis lines overexpressing obtained this study showed higher tolerance detrimental effects H2O2, MeJA, ABA treatment. In addition, resistance niger, which was linked marked increase antioxidant leaves under conditions. This correlated upregulation pathogenesis-related genes (AtPDF1.2a, AtERF1, AtVSP2, AtMYC2, AtPR1, AtACS6, AtETR1, AtLOX2) transgenic lines. Overall, our results indicate respond ubiquitously range stimuli seem be crucial for basal plants fungi. could therefore valuable target genetic engineering enhance wheat

Язык: Английский

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Advances in cheese safety and quality: harnessing irradiation technologies for enhanced preservation

Journal of Food Science and Technology, Год журнала: 2025, Номер 62(3), С. 433 - 452

Опубликована: Янв. 15, 2025

Язык: Английский

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Enhancing Antibacterial Efficacy: Synergistic Effects of Citrus aurantium Essential Oil Mixtures against Escherichia coli for Food Preservation

Foods, Год журнала: 2024, Номер 13(19), С. 3093 - 3093

Опубликована: Сен. 27, 2024

Essential oils (EOs) from various medicinal and aromatic plants are known for their diverse biological activities, including antimicrobial effects. Citrus aurantium EO is traditionally used therapeutic benefits due to its high content of bioactive compounds. Therefore, this study focuses on potential use as a food preservative by investigating the combined antibacterial properties EOs leaves (EO1), flowers (EO2), small branches (EO3) against six bacterial strains agar disk diffusion, minimum inhibitory concentration (MIC), bactericidal (MBC) methods. The chemical compositions were analysed gas chromatography–mass spectrometry (GC-MS) revealed presence numerous compounds responsible properties. MIC values 3.12 mg/mL, 4.23 1.89 EO1, EO2 EO3, respectively, while MBC 12.5 6.25 respectively. A simplex centroid design was created analyse effect individual E. coli. showed enhanced activity compared oils, suggesting synergistic (e.g., trial 9 with an 0.21 mg/mL), allowing lower concentrations reducing negative effects flavour aroma. Additionally, practical application investigated (at twice MIC) in raw chicken meat stored at 4 °C 21 days. EOs, individually combination, effectively extended shelf life inhibiting growth (total count less than 1 × 104 CFU/g treated samples 7 107 control day storage). underlines C. natural preservatives that represent sustainable effective alternative synthetic chemicals preservation.

Язык: Английский

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Insight into Pelargonium odoratissimum Essential Oil Preservative Properties Effect on Ground Beef

Foods, Год журнала: 2024, Номер 13(19), С. 3181 - 3181

Опубликована: Окт. 7, 2024

Pelargonium plants are very popular and well-known for their essential oils (EOs), which used medicinal purposes in food. This study focused on the EO of

Язык: Английский

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