Metabolic Profiling of a Mediterranean-Inspired (Poly)phenol-Rich Mixture in the Brain: Perfusion Effect and In Vitro Blood–Brain Barrier Transport Validation DOI Creative Commons
María Ángeles Ávila‐Gálvez,

Beatriz Garay-Mayol,

Alicia Marín

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 24, 2025

A Mediterranean diet rich in (poly)phenols has been linked to neuroprotection, but its effects likely depend on the ability of phenolic metabolites cross blood-brain barrier (BBB). This study evaluated kinetics plasma and brain distribution Sprague-Dawley rats following oral administration a polyphenol-rich extract mixture from foods (pomegranate, lemon, orange, grape, olive). UPLC-ESI-QTOF analyses revealed 39 phenolic-derived plasma, which 20 were nonperfused (NPB) 19 perfused brains (PB), including hydroxytyrosol tyrosol sulfates, ellagic acid, dihydrocaffeic derived metabolites. Kinetic data showed substantially higher metabolite concentrations than brain, with slightly levels NPB. The BBB transport efficiency was validated vitro using human microvascular endothelial cells (HBMECs), showing improved when tested as mixtures. These findings confirm that circulating can reach tissues, contributing preventing neurodegenerative diseases.

Язык: Английский

Enhanced brain delivery and antiproliferative activity of resveratrol using milk-derived exosomes DOI Creative Commons
María Ángeles Ávila‐Gálvez,

Beatriz Garay-Mayol,

Juan Antonio Giménez‐Bastida

и другие.

Journal of Agriculture and Food Research, Год журнала: 2024, Номер 18, С. 101370 - 101370

Опубликована: Авг. 25, 2024

The polyphenol resveratrol (RSV) undergoes extensive phase II metabolism, which limits its bioavailability and bioactivity, including anticancer effects. Growing preclinical evidence reinforces milk-derived exosomes (EXOs) as promising nanocarrier drugs bioactives delivery systems. Herein, to overcome the mentioned RSV's drawbacks, EXOs were loaded with RSV (EXO-RSV) evaluate brain in Sprague-Dawley rats vitro antiproliferative effects against human neuroblastoma SH-SY5Y glioblastoma U-87MG cancer cells compared non-encapsulated RSV. Pharmacokinetic analyses perfused brains showed detection only after EXO-RSV administration, not administration. Encapsulation also resulted lower concentrations of II-derived metabolites circulation. Additionally, blood-brain barrier (BBB) transport assays using microvascular endothelial (HBMECs) corroborated that encapsulation enhanced efficiency protected it from cellular metabolism. In silico phase-II conjugates, primarily 3-glucuronide, capacity than cross BBB. Finally, (47−750 nM) an increased dose-dependent efficacy on both cell lines (10 μM), was ineffective 72 h treatment. Our findings suggest protect metabolism enhance activity.

Язык: Английский

Процитировано

4
Exosomes: Traversing the blood-brain barrier and their therapeutic potential in brain cancer DOI
Xiaopei Zhang,

Nichole Artz,

Dennis A. Steindler

и другие.

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Год журнала: 2025, Номер unknown, С. 189300 - 189300

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Metabolic Profiling of a Mediterranean-Inspired (Poly)phenol-Rich Mixture in the Brain: Perfusion Effect and In Vitro Blood–Brain Barrier Transport Validation DOI Creative Commons
María Ángeles Ávila‐Gálvez,

Beatriz Garay-Mayol,

Alicia Marín

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 24, 2025

A Mediterranean diet rich in (poly)phenols has been linked to neuroprotection, but its effects likely depend on the ability of phenolic metabolites cross blood-brain barrier (BBB). This study evaluated kinetics plasma and brain distribution Sprague-Dawley rats following oral administration a polyphenol-rich extract mixture from foods (pomegranate, lemon, orange, grape, olive). UPLC-ESI-QTOF analyses revealed 39 phenolic-derived plasma, which 20 were nonperfused (NPB) 19 perfused brains (PB), including hydroxytyrosol tyrosol sulfates, ellagic acid, dihydrocaffeic derived metabolites. Kinetic data showed substantially higher metabolite concentrations than brain, with slightly levels NPB. The BBB transport efficiency was validated vitro using human microvascular endothelial cells (HBMECs), showing improved when tested as mixtures. These findings confirm that circulating can reach tissues, contributing preventing neurodegenerative diseases.

Язык: Английский

Процитировано

0