Foodborne Pathogens and Disease, Год журнала: 2025, Номер unknown
Опубликована: Апрель 22, 2025
This study aimed to develop a novel, refrigeration-free preservation method extend quail egg shelf life and enhance consumer safety. The antimicrobial efficacy of limonene vapor against Salmonella Enteritidis Staphylococcus aureus was evaluated. Limonene nanoemulsions (0.04%, 0.08%, 0.12%) were used generate for coating eggshells. process performed using an ultrasonic device with controllable temperature chamber maintain different temperatures: 4°C (cold), 25°C (medium), 37°C (high temperature). enhanced by applying UV irradiation 10 min. mode action analyzed through bacterial morphology, biofilm formation, cell leakage. (0.08%) at ultraviolet (UV) reduced S. 6-7 log10 on eggshells, whereas 37°C, the reductions only 3.5 2.8 log10, respectively. not detected, remained within food safety standards when eggs stored 30 ± 2°C days, compared control, which showed growth one day. Possible mechanisms suggested that cold-generated formed thin film eggshell surface. caused deformation abnormal morphology in cells induced pore formation walls, enhancing injury. exposure further exacerbated cellular damage, resulting synergistic effect. However, this did cause significant membrane damage or lead substantial leakage intracellular materials aureus. cost-effective could be adapted on-farm application before distribution, ensuring safer consumers.
Язык: Английский