Evaluation of in vitro pharmacological activities of medicinal mushrooms in the context of dry eye disease

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Март 5, 2025

Ethnic groups worldwide use mushrooms, particularly polypores (a group of fungi with woody fruiting bodies), to manage inflammatory conditions. In this study, the in vitro anti-inflammatory potential and mycochemical composition six polypore extracts derived from fruit bodies Fomes fomentarius (L.) Fr. (FF), Ganoderma lucidum (Fr.) P. Karst. (GL), tsugae Murrill (GT), Gloeophyllum odoratum (Wulfen) Imazeki (GO), Laricifomes officinalis (Vill.) Kotl. Pouzar (LO), sclerotium Inonotus obliquus Pilát (IO) were analyzed for their relevance treat dry eye disease (DED). Ethanolic fungal materials prepared chemically characterized by UHPLC-ELSD/MS TLC analyses before investigating extracts' cytotoxic, antioxidant, anti-inflammatory, lipid-stimulating properties. Radical scavenging intracellular reactive oxygen species (ROS) assays carried out UVB-exposed human corneal epithelial (HCE-T) immortalized meibomian gland (IHMGEC) cells evaluate antioxidant capacities. To examine influence processes, associated DED, a secretion assay pro-inflammatory cytokines was conducted HCE-T LPS-stimulated monocytic THP-1 cells. The lipid droplets secreted IHMGECs determine Extracts GT, GL, GO, IO found have high radical abilities. They significantly reduced ROS iHMGEC GO GL inhibited cytokine even at low concentrations. All tested (IP10, IL-6, IL-8, α) Several investigated exhibit multifaceted pharmacological activities. Due cytotoxic activity on HCE-T, iHMGEC, cells, are pertinent treatment

Язык: Английский

To Explore the Mechanism of <i>T</i><i>remella fuciformis</i> Ameliorating Constipation by Network Pharmacology Analysis and Molecular Docking

Hans Journal of Food and Nutrition Science, Год журнала: 2025, Номер 14(02), С. 214 - 229

Опубликована: Янв. 1, 2025

Язык: Английский

A study integrated metabolomics, network pharmacology, and machine learning to investigate differential metabolites of volatile compounds in food evaluation: A case of porcini mushrooms

Food Bioscience, Год журнала: 2024, Номер unknown, С. 105177 - 105177

Опубликована: Сен. 1, 2024

Язык: Английский