Network pharmacology and cell experiment demonstrate the neuroprotective potential on hypertension-induced nerve injury of hazelnut-derived peptide YYLLVR

Food Bioscience, Год журнала: 2025, Номер unknown, С. 106089 - 106089

Опубликована: Фев. 1, 2025

Язык: Английский

Immunomodulatory Potential of Kaempferol Isolated from Peronema canescens Jack. Leaves Through Inhibition of IL-6 Expression

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3068 - 3068

Опубликована: Март 27, 2025

Sungkai leaves were selected due to their herbal medicine prevalence and documented biological activities. This study explores the immunomodulatory potential of kaempferol isolated from (Peronema canescens Jack.) through a combination in silico vitro methods. P. extracted with ethanol using maceration, followed by fractionation n-hexane, ethyl acetate, water separatory funnel. Among all fractions, acetate fraction demonstrated strongest inhibitory effect on IL-6 (Interleukin 6) expression, leading further separation for enhanced analysis its activity. The resulting sub-fractions purified vacuum liquid chromatography n-hexane gradient. Sub-fraction E was preparative thin-layer obtain pure compound identified as UV, FTIR, MS, NMR analyses. then evaluated molecular docking dynamics simulations, employing MM-PBSA (Molecular Mechanics Poisson-Boltzmann Surface Area) binding affinity calculations. Kaempferol showed (ΔG) -5.98 kcal/mol, slightly stronger than TLA (tartaric acid) (-5.90 kcal/mol). Key interactions amino acid residues, such Gln175, Arg182, Arg179, observed. Additionally, simulation that exhibited better stability between 15 ns 100 ns. has strong van der Waals (-17.02 kcal/mol) electrostatic (-293.16 kcal/mol), free energy (-17.85 significantly (-1.00 stability, combined ability reduce expression vitro, highlights kaempferol's potential.

Язык: Английский

Supplements extracted from Lophatherum gracile Brongn. ameliorates hyperuricemia by regulating nucleotide metabolic enzymes and urate transporters

Food & medicine homology., Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский