Life,
Год журнала:
2023,
Номер
13(12), С. 2323 - 2323
Опубликована: Дек. 11, 2023
Consumers
today
seek
safe
functional
foods
with
proven
health-promoting
properties.
Current
evidence
shows
that
a
healthy
diet
can
effectively
alleviate
oxidative
stress
levels
and
reduce
inflammatory
markers,
thereby
preventing
the
occurrence
of
many
types
cancer,
hypertension,
cardiovascular
neurological
pathologies.
Nevertheless,
as
fruits
vegetables
are
mainly
consumed
fresh,
they
serve
vectors
for
transmission
pathogenic
microorganisms
associated
various
disease
outbreaks.
As
result,
there
has
been
surge
in
interest
microbiome
vegetables.
Therefore,
given
growing
sweet
cherries,
since
their
microbial
communities
have
largely
ignored,
primary
purpose
this
study
is
to
investigate
culturome
at
maturity
stages
first
time.
A
total
55
were
isolated
from
cherry
fruit,
comprising
23
bacteria
32
fungi
species.
Subsequently,
selected
isolates
molecularly
identified
by
amplifying
16S
rRNA
gene
ITS
region.
Furthermore,
it
was
observed
became
more
diverse
fruit
matured.
The
most
abundant
taxa
included
Pseudomonas
Ralstonia
among
bacteria,
Metschnikowia,
Aureobasidium,
Hanseniaspora
fungi.
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 10, 2025
Background
This
study
explores
the
impact
of
varying
altitudes
on
quality
characteristics
Mianning
ham.
Methods
By
utilizing
Solid-Phase
Microextraction—Gas
Chromatography–Mass
Spectrometry
(SPME-GC-MS)
technology
and
high-throughput
sequencing
techniques,
physicochemical
properties,
volatile
flavor
compounds,
shifts
in
microbial
communities
ham
at
different
were
investigated.
Results
Ham’s
water
content,
aw,
pH,
malondialdehyde
content,and
nitrite
content
high
higher,
while
salt
low
was
higher.
112
compounds
identified
fermented
for
0,
1,
2
years
altitude
altitude,
more
abundant
than
those
altitude.
The
main
1-octene-3-ol,
Nonyl
aldehyde,
Octanal,
15
other
compounds.
At
phylum
level,
dominant
bacteria
Firmicutes
Proteobacteria,
fungus
Basidiomycota.
Staphylococcus
bacterium
genus
Aspergillus
fungus.The
correlation
analysis
microorganisms
substances
showed
that
Cobetia
promoted
formation
Benzaldehyde
altitudes.
In
contrast,
Kocuria
1-Octanol,
Heptanol,1-Butanol,
2-Heptanone,
3-Hydroxy-2-butanone,
Hexanal
Discussion
There
obvious
differences
between
two
Environment Development and Sustainability,
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 2, 2024
Abstract
Two
major
challenges
in
the
modern
world
are
ensuring
food
security
and
using
sustainability
limited
areas,
face
of
climate
change
population
growth.
It
is
aimed
to
raise
awareness
on
importance
working
from
a
multidisciplinary
perspective,
together
with
developing
technology
science,
solving
current
problems/troubles,
that
simultaneous
use
bio-based
technologies
innovative
techniques
will
ensure
an
effective
role
overcoming
many
difficulties
future
generations
face,
as
well
can
be
achieved.
The
review
provides
detailed
systematic
analysis
mutually
supportive
sustainable
agricultural
system
within
framework
this
subject.
review,
which
universally
reliable
databases
were
used,
was
carried
out
information
obtained
practice
approach-based
articles
(>
4000).
fact
main
topic
has
lowest
publication
content
terms
"sustainability
biotechnology"
according
determined
keywords
indicates
need
for
additional
research
applications
contribute
literature.
Similar
aim,
it
also
been
concluded
development
multidisciplinary,
interdisciplinary
transdisciplinary
perspective
comprehensive
broader
focus
eliminating
deficiencies
fields
play
problems
related
safety
security.
Thus,
contributing
protection
should
considered
another
important
outcome
not
forgotten.
In
short,
being
aware
world,
especially
its
resources,
infinite
continuity
healthy
generations,
agriculture,
must
meticulously
protected,
developed
step
forward
by
more
strategies
field
work,
great
life.
Applied and Environmental Microbiology,
Год журнала:
2024,
Номер
90(11)
Опубликована: Ноя. 1, 2024
ABSTRACT
Fungi
generate
a
diverse
array
of
bioactive
compounds
with
significant
pharmaceutical
applications.
However,
the
chemical
diversity
natural
products
in
fungi
remains
largely
unexplored.
Here,
we
present
paradigm
for
specifically
discovering
and
from
by
integrating
genome
mining
building
block
molecular
network
coculture
analysis.
Through
pangenome
sequence
similarity
analysis,
identified
rare
type
I
polyketide
enzyme
Penicillium
sp.
ZJUT-34.
Subsequent
strategy
led
to
identification
isolation
pair
novel
polyketides,
(±)-peniphenone
E
[(±)−
1
],
three
known
polyketides
(
2–4
),
precursor
5–7
)
combined
culture
ZJUT-34
ZJUT23.
Their
structures
were
established
through
extensive
spectroscopic
including
NMR
HRESIMS.
Chiral
HPLC
separation
compound
yielded
enantiomers
(+)−
(−)−
,
their
absolute
configurations
determined
using
calculated
ECD
methods.
Compound
(±)−
is
notable
its
unprecedented
structure,
featuring
unique
2-methyl-hexenyl-3-one
moiety
fused
clavatol
core.
We
proposed
hypothetical
biosynthetic
pathway
.
Furthermore,
2
5
6
exhibited
strong
antioxidant
activity,
whereas
3
four
moderate
activity
compared
positive
control,
ascorbic
acid.
Our
research
demonstrates
pioneering
uncovering
merging
mining,
metabolomics,
cocultivation
This
approach
addresses
challenge
produced
enzymes
that
are
often
silent
under
conventional
conditions
due
gene
regulation.
IMPORTANCE
Polyketides,
particularly
those
complex
structures,
crucial
drug
development
synthesis.
study
introduces
discover
new
genomics,
strategies.
By
combining
networks,
coculturing
techniques,
isolated
polyketide,
E,
along
highlights
potential
strategies
explore
fungal
compounds.
The
successful
distinctive
effectiveness
this
integrated
method
enhancing
product
discovery
underscores
value
innovative
approaches
research.
BIO Web of Conferences,
Год журнала:
2024,
Номер
83, С. 04006 - 04006
Опубликована: Янв. 1, 2024
The
purpose
of
the
research
is
to
study
influence
various
crop
cultivation
technologies
on
structure
micromycete
complexes
and
level
microbial
soil
toxicosis.
Extensive,
organic
intensive
technologiesin
a
six-field
rotation
soddy-podzolic
gleyic
for
2021–2022
have
been
studied.
To
complex
deep
seeding
method,
spatial
occurrence
index
species,
Syorensen-Chekanovsky
similarity
coefficient
significance
indexwere
used.
determine
toxicosis,
method
initiated
community
according
V.S.
Guzev
was
According
results
experiments,
fungi
from
departments
Zygomycota
Ascomycota
were
discovered,
in
2021
–
10
2022
16
species.
In
2022,
largest
number
micromycetes
recorded
under
cereals
with
extensive
technologies,
2021,
high
variants
annual
perennial
grasses
using
same
technologies.
greatest
manifestation
toxicosis
noted
2021:
germinating
ability
test
seeds
control
–76.67-92.67%,
when
initiating
44.67-76.67%.
grain
crops
by
contributed
greater
Revista Destaques Acadêmicos,
Год журнала:
2024,
Номер
16(3)
Опубликована: Окт. 28, 2024
O
gênero
Penicillium
é
amplamente
utilizado
na
produção
de
biomoléculas
com
diversas
aplicações
industriais,
e
o
uso
linhagens
amazônicas
especialmente
importante
devido
à
sua
diversidade
genética
única,
que
pode
resultar
enzimas
propriedades
bioativas
distintas.
Assim,
objetivo
deste
trabalho
foi
investigar
a
proteínas
caracterizar
as
proteases
P.
purpurogenum
CFAM
214.
A
linhagem
fúngica
reativada
da
Coleção
Fungos
Amazônia
(CFAM),
cultivada
em
diferentes
meios
cultura,
Batata
Dextrose
Levedura
(BDL),
Yeast
Extract
Sucrose
(YES),
International
Streptomyces
Project
2
(ISP2)
Sabouraud
(SB),
foram
subsequentemente
analisados
por
Cromatografia
Líquida
acoplado
ao
Espectrômetro
Massas
(LC-MS).
Entre
esses,
um
meio
cultura
selecionado
realizada
curva
durante
168
horas.
Além
disso,
analisado
perfil
proteico
eletroforese
unidimensional.
efeito
do
pH
temperatura
atividade
proteolítica
avaliado
variando
essas
condições.
Os
dados
análise
variância
(ANOVA)
teste
Tukey.
cromatográfica
revelou
SB
induziu
maior
biomoléculas,
seguido
YES
BDL.
No
SB,
totais
atingiu
pico
1,7
µg/µL
120
unidimensional
indicou
extracelulares
predominantes
entre
50
150
kDa.
máxima
104,23
U/mL
também
horas
apresentaram
estabilidade
8
50°C.
Portanto,
esses
resultados
indicam
214
uma
fonte
promissora
proteases,
potencial
para
industriais
nas
áreas
limpeza,
alimentos,
medicamentos
têxteis.
