Differential heat shock protein responses in two species of Pacific salmon and their utility in identifying heat stress DOI Creative Commons
Vanessa R. von Biela, Amy M. Regish, Lizabeth Bowen

и другие.

Conservation Physiology, Год журнала: 2023, Номер 11(1)

Опубликована: Янв. 1, 2023

Rapid and accelerating warming of salmon habitat has the potential to lower productivity Pacific (Oncorhynchus species) populations. Heat stress biomarkers can indicate where is most likely affecting fish populations; however, we often lack clear classifications that separate individuals with without heat needed make these tools operational. We conducted a exposure experiment trials lasting 12 or 36 h using juvenile Chinook tshawytscha) coho kisutch) validate in white muscle. Following habituation 13°C, were exposed water temperatures increased 15°C, 17°C, 19°C, 21°C 23°C. shock protein 70 abundance (HSP70 measured by ELISA) transcription 13 genes (mRNA qPCR) including three (hsp70, hsp90, hsp27) measured. A distinct response was apparent 23°C HSP70. threshold for classification > 2 ng HSP70 mg.1 total identified 100% 21 treated compared 4% cooler treatments. For salmon, 3 Transcription from panel separated between stressful temperature experiences (≥21°C ≥23°C salmon) ~ 85% correct classification. Our findings more temperature-sensitive than support use sampled muscle assessing individual wild an option non-lethal biopsies spawning adults.

Язык: Английский

Fishway performance of adult Chinook salmon completing one of the world's longest inland salmon migrations to the upper Yukon River DOI
William M. Twardek, Steven J. Cooke,

N. W. R. Lapointe

и другие.

Ecological Engineering, Год журнала: 2022, Номер 187, С. 106846 - 106846

Опубликована: Ноя. 21, 2022

Язык: Английский

Процитировано

7
Intestinal lesions and parasites associated with senescence and prespawn mortality in Chinook Salmon (Oncorhynchus tshawytscha) DOI Creative Commons

Stephanie Nervino,

Tamsen Polley,

James T. Peterson

и другие.

Journal of Fish Diseases, Год журнала: 2023, Номер 47(2)

Опубликована: Окт. 27, 2023

Abstract Prespawn mortality (PSM) presents a major problem for the recovery of spring Chinook Salmon ( Oncorhynchus tshawytscha ) populations. In Willamette River, Oregon, PSM exceeds 90% in some years but factors explaining it are not well understood. We examined intestinal tissue samples using histological slides from over 783 collected between 2009 and 2021, which included tissues fish, artificially spawned captive broodstock (BS) normal river run comprised trapped (Live) naturally post‐spawned (RPS) fish river. observed degeneration epithelium loss villous structure, with concurrent severe enteritis. A natural progression decline epithelial integrity (EI) through summer fall until spawning subsequent death was also observed. Live exhibited high EI scores (mean = 68%), BS variable (35%) RPS (14%). prominent (13%), very similar to despite having been earlier year. Hence, we argue that low strongly linked PSM. Ceratonova shasta Enterocytozoon schreckii were common all groups, neither either or EI.

Язык: Английский

Процитировано

4
Behavioural responses to acute warming precede critical shifts in the cellular and physiological thermal stress responses in fish DOI Creative Commons
Travis Durhack, Matt J. Thorstensen, Theresa E. Mackey

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 1, 2024

From a conservation perspective, it is important to identify when sub-lethal temperatures begin adversely impact an organism. However, unclear whether, during acute exposures, these cellular thresholds occur at similar other physiological or behavioural changes. To test this, we estimated temperature preference (15.1 ± 1.1 °C) using shuttle box, thermal optima for aerobic scope (10—15 respirometry, agitation (22.0 1.4 as the point where fish exhibits avoidance response and CTmax (28.2 0.4 upper limit 1 yr old Brook Trout (Salvelinus fontinalis) acclimated 10 °C. We then acutely exposed different subset of sampled tissues they reached target after 60 min recovery used qPCR estimate mRNA transcript levels genes associated with heat shock proteins, oxidative stress, apoptosis, inducible transcription factors. A major shift in transcriptome occurred near temperature, which may link between stress response.

Язык: Английский

Процитировано

1
Predicting favourable streams for anadromous salmon spawning and natal rearing under climate change DOI Creative Commons
Josephine C. Iacarella, J. Daniel Weller

Canadian Journal of Fisheries and Aquatic Sciences, Год журнала: 2023, Номер 81(1), С. 1 - 13

Опубликована: Авг. 17, 2023

Successful management of imperilled anadromous salmon stocks requires understanding how will respond to a changing climate across their extensive ranges. Though largely unused for date, environmental niche models (ENMs) can provide strong foundation estimating where and much habitat likely remain favourable. We applied comprehensive suite ENM statistical approaches five Pacific species, focusing on freshwater spawning natal rearing as not only critical population survival, but also highly susceptible climate. developed ENMs ranges British Columbia (BC), Canada, western US, projected future scenarios BC. Projections indicated net expansion favourable species in BC, with the most change occurring by 2060. However, shifts varied greatly geographically notable reductions coastal southern interior streams. Our results basis identifying important changes conditions help inform priorities restoration protection actions.

Язык: Английский

Процитировано

3
Differential heat shock protein responses in two species of Pacific salmon and their utility in identifying heat stress DOI Creative Commons
Vanessa R. von Biela, Amy M. Regish, Lizabeth Bowen

и другие.

Conservation Physiology, Год журнала: 2023, Номер 11(1)

Опубликована: Янв. 1, 2023

Rapid and accelerating warming of salmon habitat has the potential to lower productivity Pacific (Oncorhynchus species) populations. Heat stress biomarkers can indicate where is most likely affecting fish populations; however, we often lack clear classifications that separate individuals with without heat needed make these tools operational. We conducted a exposure experiment trials lasting 12 or 36 h using juvenile Chinook tshawytscha) coho kisutch) validate in white muscle. Following habituation 13°C, were exposed water temperatures increased 15°C, 17°C, 19°C, 21°C 23°C. shock protein 70 abundance (HSP70 measured by ELISA) transcription 13 genes (mRNA qPCR) including three (hsp70, hsp90, hsp27) measured. A distinct response was apparent 23°C HSP70. threshold for classification > 2 ng HSP70 mg.1 total identified 100% 21 treated compared 4% cooler treatments. For salmon, 3 Transcription from panel separated between stressful temperature experiences (≥21°C ≥23°C salmon) ~ 85% correct classification. Our findings more temperature-sensitive than support use sampled muscle assessing individual wild an option non-lethal biopsies spawning adults.

Язык: Английский

Процитировано

3