Differentiating Tangerine Peels from Other Citrus reticulata through GC-MS, UPLC-Q-Exactive Orbitrap-MS, and HPLC-PDA DOI Creative Commons
Zhen Song,

Jiepei Xu,

Jingyuan Tian

и другие.

ACS Omega, Год журнала: 2024, Номер 10(1), С. 1688 - 1704

Опубликована: Дек. 27, 2024

The nonvolatile and volatile compounds in the peels of 13 Citrus reticulata cultivars (4 mandarins, 5 tangerines, 4 hybrids) sinensis (sweet oranges) were analyzed. Initially, 66 detected using gas chromatography–mass spectrometry (GC-MS). Tangerines distinguished from other citrus (mandarins, sweet oranges, by having higher oil extraction rates relative contents o-Cymene, α-Terpinene, d-α-Pinene, Terpinolene, γ-Terpinene, l-β-Pinene, 3-Thujene. Additionally, 115 tentatively identified ultraperformance liquid chromatography-Q-Exactive Orbitrap tandem mass (UPLC-Q-Exactive Orbitrap-MS). C. contained fewer than did reticulata. Pterostilbene was all tangerines but not mandarins hybrids, suggesting its potential as a marker compound for differentiating Lastly, high-performance chromatography-photodiode array (HPLC-PDA) used to quantify 9 major components. Heat map principal component analysis showed that differed hybrids). It may be caused content synephrine, nobiletin, tangeretin, 5-hydroxy-6,7,8,3′,4′-pentamethoxyflavone tangerines. study obtain information application different types (tangerines, or peels, thereby promoting their recycling.

Язык: Английский

Astragaloside IV alleviates heatstroke brain injury and neuroinflammation in male mice by regulating microglial polarization via the PI3K/Akt signaling pathway DOI Open Access

Zeze Wang,

Zhen Luo, Yulong Tan

и другие.

Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 180, С. 117545 - 117545

Опубликована: Окт. 14, 2024

Язык: Английский

Процитировано

4
Euodiae Fructus: a review of botany, application, processing, phytochemistry, quality control, pharmacology, and toxicology DOI Creative Commons

Yule Hao,

Jiawen Qi,

Xinggui Huang

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Янв. 29, 2025

Euodiae Fructus (EF) is the dried and nearly ripe fruit of Euodia rutaecarpa , first recorded in Shen Nong’s Herbal Classic . EF a versatile Traditional Chinese Medicine (TCM) known for effects dispelling colds alleviating pain, suppressing adverse qi to relieve vomiting, boosting yang mitigate diarrhea. However, it should be noted that possesses mild toxicity. In TCM prescriptions, employed treat various ailments, including abdominal diarrhea, chronic non-atrophic gastritis, irritable bowel syndrome, primary dysmenorrhea. This review collected literature published before September 2024 on EF. An exhaustive analysis was conducted utilizing multiple sources, namely classic books scientific databases like Web Science, PubMed, Elsevier, ACS, ResearchGate, Google Scholar, National Knowledge Infrastructure. So far, more than 300 metabolites have been extracted identified from EF, exhibiting pharmacological effects, such as cardiovascular protection, gastrointestinal neuroprotection, anti-inflammation, analgesia, anti-tumor, glucose lipid metabolism regulation, etc. It also exhibits diverse toxicological properties poses specific toxic risks liver, heart, kidney. Nonetheless, research scarce regarding toxicology especially its cardiotoxicity nephrotoxicity. Further in-depth necessary explore mechanisms underlying EF’s develop strategies quality control toxicity mitigation. The can reduced by processing, but this aspect rarely discussed, needs further standardized. Evodiamine, rutaecarpine, limonin are effective one causes evodiamine rutaecarpine intensely studied, there few studies other Therefore, paper focuses botanical characteristics, traditional applications, processing methods, phytochemistry, control, pharmacology, We hope provides theoretical basis future high-value high-connotation development

Язык: Английский

Процитировано

0
Rotenone Induces Parkinsonism with Constipation Symptoms in Mice by Disrupting the Gut Microecosystem, Inhibiting the PI3K-AKT Signaling Pathway and Gastrointestinal Motility DOI Open Access
Liu Li, Yan Zhao,

Weixing Yang

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 2079 - 2079

Опубликована: Фев. 27, 2025

Parkinson's disease (PD) is one of the most common neurodegenerative diseases. Constipation a prodromal symptom PD. It important to investigate pathogenesis constipation symptoms in Rotenone has been successfully used establish PD animal models. However, specific mechanism rotenone-induced not well understood. In this work, we found that appeared earlier than motor impairment mice gavaged with low dose rotenone (30 mg/kg·BW). only caused loss dopaminergic neurons and accumulation α-synuclein, but also significantly reduced serum 5-HT levels 5-HTR4 striatum colon. The mRNA expression aquaporins, gastrointestinal motility factors (c-Kit, Cx43, smMLCK MLC-3) mouse colon was regulated by rotenone. addition, both brain showed inflammation barrier dysfunction; PI3K/AKT pathway substantia nigra inhibited Importantly, structure, composition function gut microbiota were altered Some taxa closely associated symptoms, inflammation, status mice. Akkermansia, Staphylococcus Lachnospiraceae_UCG-006 may play role exacerbating whereas Acinetobacter, Lactobacillus, Bifidobacterium, Solibacillus Eubacterium_xylanophilum_groups be beneficial stimulating peristalsis, maintaining alleviating damage conclusion, low-dose can cause parkinsonism disrupting intestinal microecosystem inhibiting PI3K-AKT motility.

Язык: Английский

Процитировано

0
The Improvement Effects of Sika Deer Antler Protein in an Alzheimer's Disease Mouse Model via the Microbe–Gut–Brain Axis DOI Creative Commons

Lei Li,

Lulu Wang, Weixing Ding

и другие.

Food Science & Nutrition, Год журнала: 2024, Номер 13(1)

Опубликована: Дек. 30, 2024

ABSTRACT Alzheimer's disease (AD) is a neurodegenerative disorder of the central nervous system. The interplay between intestinal microbiota and metabolites believed to influence brain function pathogenesis conditions through microbe–gut–brain axis. Sika deer antler protein possesses neuroprotective properties; however, precise mechanism by which it improves AD remains unclear. ameliorated in vivo activating phosphatidylinositol 3‐kinase (PI3K)/protein kinase B (AKT)/nuclear factor erythroid 2–related 2 (Nrf2) signaling pathway. metabolome tissues contents were tested analyzed according microbe‐gut‐brain theory. increased beneficial bacterial levels decreased harmful levels. Correlation analyses using gut flora–metabolomics pathway ultimately revealed that sika modulated tract bi‐directionally via tyrosine metabolism pathway, thereby establishing connection within Kyoto Encyclopedia Genes Genomes (KEGG) analysis differential metabolite targets DAP4 group showed enriched pathways mainly included PI3K/AKT, was consistent with findings pharmacodynamic mechanisms observed experiments. This suggests may be involved interactions improve PI3K/AKT/Nrf2 These add our understanding axis facilitated offer novel insights for further research on alleviating AD.

Язык: Английский

Процитировано

2
Mechanisms by which Ganglioside GM1, a specific type of glycosphingolipid, ameliorates BMAA-induced neurotoxicity in early-life stage of zebrafish embryos DOI
Yanjun Liu, Yanan Zhu, Xiaoxu Wang

и другие.

Food Research International, Год журнала: 2024, Номер 200, С. 115502 - 115502

Опубликована: Дек. 9, 2024

Язык: Английский

Процитировано

0
Exploring the key role of neuroinflammation in the formation of delayed encephalopathy after acute carbon monoxide poisoning based on multi-omics and snRNA-seq DOI Creative Commons
Jinlan Li, Jun Li, Jachih Fu

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Дек. 10, 2024

Abstract Despite the high incidence and disability rates of delayed encephalopathy after acute carbon monoxide poisoning (DEACMP), its pathogenesis remains enigmatic, specific predictive markers are lacking. This study aimed to elucidate molecular underpinnings identify biomarkers DEACMP through multi-omics single-nucleus RNA sequencing (snRNA-seq). We collected clinical data blood samples from 105 participants, including healthy controls (HCs), patients (ACOP), those receiving comprehensive treatment for ACOP (ACOP-CT). Untargeted metabolomics was employed profile serum metabolites across these groups. Additionally, individuals HCs, ACOP, non-delayed (DEACMP-N), groups (n = 3 each) were randomly selected transcriptome potential targets pivotal signaling pathways associated with DEACMP. Furthermore, we established severe Control Sprague-Dawley rat models assessed neurocognitive function using Morris water maze on 28th day. Subsequently, three rats Control, DEACMP, + Dexamethasone Selenomethionine snRNA-seq analyze hippocampal single-cell transcriptional profiles. Immunofluorescence multiplexing then performed validate identified targets. Our analysis participants highlights role inflammation in influencing prognosis poisoning. Metabolomics 19 that significantly differed between DEACMP-N compared ACOP-CT follow-up results. Transcriptomics 12 indicated is primarily six pathways, lysosome tuberculosis. Given microglia central nervous system immune effectors, our revealed altered genes expression during KEGG highlighting phagosome, neutrophil extracellular trap formation, lysosome, tuberculosis as predominant pathways. Differential gene 28 differentially expressed The STRING database immunomultiplexing confirmed IFNGR1/STAT1/CTSS axis provides a overview metabolite expression, differential patients, offering robust theoretical foundation understanding

Язык: Английский

Процитировано

0
Differentiating Tangerine Peels from Other Citrus reticulata through GC-MS, UPLC-Q-Exactive Orbitrap-MS, and HPLC-PDA DOI Creative Commons
Zhen Song,

Jiepei Xu,

Jingyuan Tian

и другие.

ACS Omega, Год журнала: 2024, Номер 10(1), С. 1688 - 1704

Опубликована: Дек. 27, 2024

The nonvolatile and volatile compounds in the peels of 13 Citrus reticulata cultivars (4 mandarins, 5 tangerines, 4 hybrids) sinensis (sweet oranges) were analyzed. Initially, 66 detected using gas chromatography–mass spectrometry (GC-MS). Tangerines distinguished from other citrus (mandarins, sweet oranges, by having higher oil extraction rates relative contents o-Cymene, α-Terpinene, d-α-Pinene, Terpinolene, γ-Terpinene, l-β-Pinene, 3-Thujene. Additionally, 115 tentatively identified ultraperformance liquid chromatography-Q-Exactive Orbitrap tandem mass (UPLC-Q-Exactive Orbitrap-MS). C. contained fewer than did reticulata. Pterostilbene was all tangerines but not mandarins hybrids, suggesting its potential as a marker compound for differentiating Lastly, high-performance chromatography-photodiode array (HPLC-PDA) used to quantify 9 major components. Heat map principal component analysis showed that differed hybrids). It may be caused content synephrine, nobiletin, tangeretin, 5-hydroxy-6,7,8,3′,4′-pentamethoxyflavone tangerines. study obtain information application different types (tangerines, or peels, thereby promoting their recycling.

Язык: Английский

Процитировано

0