Enzyme Immobilization with Plant-based Polysaccharides through Complex Coacervation

LWT, Год журнала: 2025, Номер 219, С. 117537 - 117537

Опубликована: Фев. 15, 2025

Plant-based polysaccharides (PSs) were used to immobilize phytase in a coacervate system. Molecular docking predicted the intermolecular interactions and conformations between polysaccharide correlated them activity recovery of complex. PSs with two different functional groups, sulfate (iota (IC), lambda (LC), kappa (KC) carrageenan) carboxylate (low methoxyl pectin (LMP) sodium alginate (SA)) investigated. The optimized conditions for coacervation pH 4 phytase-to-polysaccharide volume ratio 12:1. Zeta potential measurements, FTIR spectroscopy, molecular confirmed that electrostatic hydrogen bonding main driving forces formation. Coacervate complexes formed LMP, SA, or KC showed high retention after immobilization, approximately 30% yield complex 75% immobilization efficiency phytase. lower enzyme observed IC LC is attributed these binding enzyme's active site. Overall, this work contributes body knowledge about can serve as guide formulating stable, ingredients plant-based diet.

Язык: Английский

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Development of Phytase Targeted PLGA Nanoparticles Loaded with Albendazole Sulfoxide and Praziquantel and Evaluation of Efficacy on the Hydatid Cysts

Journal of Drug Delivery Science and Technology, Год журнала: 2025, Номер 105, С. 106662 - 106662

Опубликована: Янв. 31, 2025

Язык: Английский

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Improving Thermal and Gastric Stability of Phytase via pH Shifting and Coacervation: A Demonstration of Bayesian Optimization for Rapid Process Tuning

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Апрель 20, 2025

Abstract Phytase (phyA) breaks down phytate, which can help with nutrient absorption in a plant-based seed diet or high-phytate food. Unfortunately, it is prone to denaturation at food preparation temperatures and easily inactivated by pepsin during gastric digestion. To protect phyA for use high-temperature processes (100 °C) digestion, chitosan (CS) was used complex phyA. Bayesian optimization, machine learning technique, demonstrate how expedite the optimization process. Thermal stability of optimized increased from 20% (Control: native state) up 74% 4:1 CS (CS-phyA) 52% 1:1 CS-phyA as measured phytase activity assay. Chitosan complexation also improved retention enzyme after thermal digestion 13-fold, retaining residual 40% 22% complexes compared itself, only retained 3% activity. Molecular docking circular dichroism were investigate underlying interaction mechanism between secondary structure heat treatment. Confocal laser scanning microscopy (CLSM) electron (SEM) confirmed revealed morphology. With stability, there great potential efficiently expanding applications high matrix. Graphical Highlights using pH shifting efficiency (BO) promising tool conditions

Язык: Английский

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Catalytic Properties of Immobilized Phytase of Silvania hatchlandensis FG 3.9.1

Applied Biochemistry and Microbiology, Год журнала: 2025, Номер 61(1), С. 84 - 91

Опубликована: Фев. 1, 2025

Язык: Английский

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Food industry side streams: an unexploited source for biotechnological phosphorus upcycling

Current Opinion in Biotechnology, Год журнала: 2024, Номер 90, С. 103209 - 103209

Опубликована: Сен. 25, 2024

Язык: Английский

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