Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 17, 2025
In
this
article,
a
dual-signal
sensor
for
the
fluorescence
and
colorimetric
detection
of
glyphosate
(Gly)
is
developed
based
on
DNAzyme-mediated
click
chemistry
DNAzyme-regulated
CeO2
peroxidase-like
activity.
DNAzyme
can
bind
to
Cu+,
triggering
reaction
between
3-Azido-7-hydroxycoumarin
(AHC)
3-ethyn-1-ol
(BOL),
thus
generating
strong
signal
at
475
nm.
Due
coordination
Gly
Cu2+,
amount
reduced
Cu+
decreases,
resulting
in
weakening
fluorescence.
addition,
inhibit
catalytic
site
enzyme
activity,
while
DNAzyme,
which
does
not
participate
reaction,
be
adsorbed
by
CeO2,
further
inhibiting
activity
reducing
oxidation
color
change
3,3′,5,5′-tetramethylbenzidine
(TMB).
The
limit
dual-mode
sensing
platform
0.15
μg/mL,
0.19
μg/mL.
This
method
has
been
successfully
applied
tap
water
soybeans,
promising
application
pesticide
residue
detection.
