Fabrication of Porous Proteinaceous Microspheres via One-Step Pickering Double Emulsions: Controllable Structure and Interfacial Cascade Biocatalysis

Langmuir, Год журнала: 2025, Номер unknown

Опубликована: Март 17, 2025

Methods based on double emulsions for producing porous microspheres have gained popularity as an effective and adaptable strategy. However, these are frequently composed of organic polymers that lack sufficient mechanical strength. Additionally, the conventional two-step process use surfactants present notable challenges. A promising solution is to replace traditional with inorganic particles, utilizing a Pickering emulsion approach. Herein, we introduced one-step approach creating emulsions, followed by straightforward solvent evaporation produce proteinaceous microspheres. By harnessing enhanced stability can manipulate morphology pore structure varying oil-(ethanol/water) volume ratio, size type emulsifier, ripening time, rotary temperature, addition enzymes. Furthermore, innovatively proposed coencapsulation glucose oxide (GOx) horseradish peroxidase (HRP) interfacial cascade catalysis, showing excellent catalytic activity, recovery, reusability. This study presents new, scalable using emulsion. It demonstrates significant potential biocatalysis, expected be applied in fields such medicine, drug delivery, biotechnology due their suitability encapsulating bioactive materials.

Язык: Английский

Pickering emulsions stabilized by protein/polysaccharide polyelectrolyte complexes for lipase catalysis

Food Chemistry, Год журнала: 2025, Номер unknown, С. 143969 - 143969

Опубликована: Март 1, 2025

Язык: Английский