The Effects of a Microbial Enzyme Mixture on Macronutrient Hydrolysis in a Static Simulation of Oro-Gastric Digestion That Models Human Digestive Senescence

Foods, Год журнала: 2025, Номер 14(6), С. 937 - 937

Опубликована: Март 10, 2025

Observational studies have shown that human digestive function declines naturally with age. Oral enzyme supplementation is a candidate strategy to enhance macronutrient digestion in older adults. The objective of this study was test the effects mixture six microbial preparations (ENZ) on nutrient bioaccessibility from mixed meal an vitro model senescence. included chicken meat, peas, and potatoes. INFOGEST 2.0 static simulation oro-gastric used physiology along consensus protocol aging. Analytical testing gastric digesta measurements free amino nitrogen (FAN), acid (AA), fatty (FA), glycerol, maltose, glucose concentrations. Peptide distribution profiles were evaluated by size exclusion chromatography (SEC) gel electrophoresis. After simulating meal, all outcomes compared pepsin-only controls, except further enhanced ENZ aging condition standard (FAN: 77.1 vs. 39.3%; essential AA: 100.4 57.6%; total FA: 12.8- 8.0-fold; maltose: 142.1 0.7%). SEC confirmed ENZ's proteolytic capacity generate more lower molecular weight peptides AAs conditions pepsin alone. Gel electrophoresis enhancement ENZ. These data showcase hydrolytic activity toward macronutrients suggest compensate for reduced aging-adapted simulation.

Язык: Английский

Development of soft food gels from high-methyl pectin-protein conjugates via acid- and heat-induced crosslinking for dysphagia-friendly applications

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142139 - 142139

Опубликована: Март 1, 2025

Язык: Английский