Molecules,
Год журнала:
2023,
Номер
28(23), С. 7889 - 7889
Опубликована: Дек. 1, 2023
In
this
study,
a
fluorescence
resonance
energy
transfer
(FRET)-based
aptasensor
for
the
detection
of
aflatoxin
B1
(AFB1)
was
designed
using
carboxyfluorescein
(FAM)-labeled
aptamer
and
short
complementary
DNA
(cDNA)
labeled
with
low
molecular
quencher
RTQ1.
The
sensing
principle
based
on
restored
FAM-aptamer
due
to
ligand-induced
displacement
cDNA
in
presence
AFB1,
leading
destruction
aptamer/cDNA
duplex
preventing
convergence
FAM
RTQ1
at
effective
FRET
distance.
Under
optimal
conditions,
linear
correlation
obtained
between
intensity
AFB1
concentration
range
2.5–208.3
ng/mL
limit
assay
equal
0.2
ng/mL.
time
30
min.
proposed
has
been
successfully
validated
by
analyzing
white
wine
corn
flour
samples,
recovery
ranging
from
76.7%
91.9%
84.0%
86.5%,
respectively.
This
work
demonstrates
possibilities
as
an
easily
accessible
tool
sensitive
detection.
homogeneous
is
appropriate
choice
contaminant
screening
complex
matrices.
Food Additives & Contaminants Part A,
Год журнала:
2024,
Номер
41(4), С. 438 - 459
Опубликована: Фев. 26, 2024
This
review
explores
the
repercussions
of
mycotoxin
contamination
in
food
and
feed,
emphasising
potential
threats
to
agriculture,
animal
husbandry
public
health.
The
primary
objective
is
make
a
comprehensive
assessment
neurotoxic
consequences
exposure,
an
aspect
less
explored
current
literature.
Emphasis
placed
on
prominent
mycotoxins,
including
aflatoxins,
fumonisins,
zearalenone
(ZEA)
ochratoxins,
known
for
inducing
acute
chronic
diseases
such
as
liver
damage,
genetic
mutation
cancer.
To
elucidate
effects,
studies
were
conducted,
revealing
association
between
exposure
neurological
damage.
encompasses
impairments
learning
memory,
motor
alterations,
anxiety
depression.
underlying
mechanisms
involve
oxidative
stress,
disrupting
balance
reactive
oxygen
species
(ROS)
antioxidant
capacity.
stress
linked
neuronal
brain
inflammation,
neurochemical
imbalance,
subsequent
behavioural
changes.
underscores
need
preventive
measures
against
exposure.
While
complete
avoidance
ideal,
exploration
into
use
antioxidants
viable
solution
discussed,
given
widespread
many
products.
Specifically,
protective
role
natural
compounds,
polyphenols,
highlighted,
showcasing
their
efficacy
mitigating
mycotoxicosis
central
nervous
system
(CNS),
evidenced
by
findings
various
models.
In
summary,
countering
mycotoxin-induced
neurotoxicity
requires
multifaceted
approach.
identified
compounds
show
promise,
but
practical
hinges
factors
like
bioavailability,
toxicity
understanding
action.
Extensive
research
crucial,
considering
diverse
responses
different
mycotoxins
conditions.
Successful
implementation
relies
specific
mycotoxin(s)
involved
achievable
effective
concentrations.
Further
clinical
trials
are
imperative
establish
safety
these
applications.
Toxics,
Год журнала:
2024,
Номер
12(5), С. 365 - 365
Опубликована: Май 15, 2024
Food
plants
are
the
basis
of
human
nutrition,
but,
in
contaminated
places,
they
can
uptake
contaminants.
Environmental
contamination
and
climate
change
modify
food
quality;
generally,
have
a
negative
impact
on
imply
risks
to
health.
Heavy
metals,
like
lead,
arsenic,
cadmium,
chromium,
be
present
at
various
environmental
levels
(soil,
water,
atmosphere),
widely
distributed
world.
carry
out
heavy
metal
bioaccumulation,
defense
pathway
for
plants,
which
is
different
every
plant
species.
Accumulation
frequent
roots
leaves,
metals
fruits
seeds;
As
Cd
always
present.
In
addition,
other
contaminants
bioaccumulate
including
emerging
contaminants,
persistent
organic
pollutants
(POPs),
pesticides,
microplastics.
these
but
also
leaves
fruits,
depending
their
chemical
structure.
The
literature
published
recent
years
was
examined
understand
distribution
among
plants.
literature,
old
agronomical
practices
new
integrated
technology
clean
control
soil,
monitor
crops
been
proposed
mitigate
produce
high
quality
safety.
Quality Assurance and Safety of Crops & Foods,
Год журнала:
2024,
Номер
16(3), С. 82 - 110
Опубликована: Авг. 31, 2024
Mycotoxins
are
poison
that
filamentous
fungi
generate
under
specific
conditions.
in
food
and
feed
have
a
detrimental
effect
on
both
human
animal
health,
resulting
significant
financial
losses
for
agriculture
sector.
Despite
the
continuing
advancement
of
traditional
approaches,
modern
research
trends
favor
novel
alternatives.
Therefore,
it
is
crucial
to
prevent
mycotoxin
contamination,
which
has
raised
concerns
around
globe.
Recent
advancements
management
contamination
been
possible
by
application
promising
new
nanomaterials.
negative
impacts
but
nanotechnology
methods
appear
be
viable,
efficient,
affordable
solutions.
This
review
elucidates
information
incidence
toxicology
mycotoxins.
Nanotechnology’s
potential
removal
mycotoxins
mentioned
briefly.
Then,
attention
directed
using
newly
developed
nanomaterials
regulate
such
as
testing,
production,
inhibition,
adsorption,
The
issues
regarding
toxicity,
incidence,
tentatively
presented
along
with
prospects
remove
from
feed.
Molecules,
Год журнала:
2023,
Номер
28(23), С. 7889 - 7889
Опубликована: Дек. 1, 2023
In
this
study,
a
fluorescence
resonance
energy
transfer
(FRET)-based
aptasensor
for
the
detection
of
aflatoxin
B1
(AFB1)
was
designed
using
carboxyfluorescein
(FAM)-labeled
aptamer
and
short
complementary
DNA
(cDNA)
labeled
with
low
molecular
quencher
RTQ1.
The
sensing
principle
based
on
restored
FAM-aptamer
due
to
ligand-induced
displacement
cDNA
in
presence
AFB1,
leading
destruction
aptamer/cDNA
duplex
preventing
convergence
FAM
RTQ1
at
effective
FRET
distance.
Under
optimal
conditions,
linear
correlation
obtained
between
intensity
AFB1
concentration
range
2.5–208.3
ng/mL
limit
assay
equal
0.2
ng/mL.
time
30
min.
proposed
has
been
successfully
validated
by
analyzing
white
wine
corn
flour
samples,
recovery
ranging
from
76.7%
91.9%
84.0%
86.5%,
respectively.
This
work
demonstrates
possibilities
as
an
easily
accessible
tool
sensitive
detection.
homogeneous
is
appropriate
choice
contaminant
screening
complex
matrices.
