Characterization of a GH43 Bifunctional Glycosidase from Endophytic Chaetomium globosum and Its Potential Application in the Biotransformation of Ginsenosides
BioTech,
Год журнала:
2025,
Номер
14(1), С. 18 - 18
Опубликована: Март 12, 2025
The
GH43
family
of
glycosidases
represents
an
important
class
industrial
enzymes
that
are
widely
utilized
across
the
food,
pharmaceutical,
and
various
other
sectors.
In
this
study,
we
identified
a
glycoside
hydrolytic
enzyme,
Xyaf313,
derived
from
plant
endophytic
fungus
Chaetomium
globosum
DX-THS3,
which
is
capable
transforming
several
common
ginsenosides.
enzyme
function
analysis
reveals
Xyaf313
exhibits
dual
functionality,
displaying
both
α-L-arabinofuranosidase
β-D-xylosidase
activity.
When
acting
as
α-L-arabinofuranosidase,
achieves
optimal
activity
23.96
U/mg
at
temperature
50
°C
pH
7.
contrast,
its
results
in
slight
reduction
to
23.24
U/mg,
with
similar
conditions
those
observed
for
Furthermore,
demonstrates
considerable
resistance
most
metal
ions
chemical
reagents.
Notably,
while
maximum
occurs
°C,
it
maintains
high
room
(30
°C),
relative
exceeding
90%.
Measurements
ginsenoside
transformation
show
can
convert
ginsenosides
Rc,
Rb1,
Rb2,
Rb3
into
Rd,
underscoring
potential
pharmaceutical
applications.
Overall,
our
findings
contribute
identification
new
bifunctional
hydrolases,
highlight
significance
fungi
promising
resource
screening
carbohydrate-decomposing
enzymes,
present
candidate
biotransformation
Язык: Английский
The emerging role of honeysuckle flower in inflammatory bowel disease
Frontiers in Nutrition,
Год журнала:
2025,
Номер
12
Опубликована: Март 28, 2025
Crohn's
disease
(CD)
and
ulcerative
colitis
(UC),
referred
to
as
inflammatory
bowel
(IBD),
pose
considerable
challenges
in
treatment
because
they
are
chronic
conditions
that
easily
relapse.
The
occurrence
of
IBD
continues
rise
developing
countries.
Nonetheless,
the
existing
therapies
for
have
limitations
fail
address
needs
patients
thoroughly.
There
is
an
increasing
need
new,
safe,
highly
effective
alternative
medications
patients.
Traditional
Chinese
Medicine
(TCM)
employed
drug
development
management
due
its
wide-range
biological
activities,
minimal
toxicity,
limited
side
effects.
Extensive
research
has
shown
certain
TCM
exhibits
significant
therapeutic
benefits
treatments.
Honeysuckle
(Lonicera
japonica)
was
used
clinical
settings
IBD.
Bioactive
metabolites
L.
japonica,
such
luteolin,
quercetin,
cyanidin,
chlorogenic
acid
(CGA),
caffeic
(CA),
saponin,
exhibit
managing
honeysuckle
flower
a
potential
candidate
anti-inflammatory,
immune
system-regulating,
antioxidant
qualities.
This
paper
reviews
It
discusses
fundamental
mechanism
japonica
bioactive
prevention
Язык: Английский