Irisin-loaded electrospun core-shell nanofibers as calvarial periosteum accelerate vascularized bone regeneration by activating the mitochondrial SIRT3 pathway DOI Creative Commons
Xi Hua,

Mingzhuang Hou,

Lei Deng

и другие.

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Июль 26, 2023

Abstract The scarcity of native periosteum poses a significant clinical barrier in the repair critical-sized bone defects. challenge enhancing regenerative potential healing is further compounded by oxidative stress at fracture site. However, introduction artificial has demonstrated its ability to promote regeneration through provision appropriate mechanical support and controlled release pro-osteogenic factors. In this study, polylactic acid (PLLA)/hyaluronic (HA)-based nanofibrous membrane was fabricated using coaxial electrospinning technique. incorporation irisin into core-shell structure PLLA/HA nanofibers (PLLA/HA@Irisin) achieved sustained release. vitro experiments that PLLA/HA@Irisin membranes exhibited favorable biocompatibility. osteogenic differentiation marrow mesenchymal stem cells (BMMSCs) improved PLLA/HA@Irisin, as evidenced increase alkaline phosphatase (ALP) activity matrix mineralization. Mechanistically, significantly enhanced mitochondrial function BMMSCs via activation sirtuin 3 antioxidant pathway. To assess therapeutic effectiveness, were implanted situ calvarial defects rats. results four eight weeks post-surgery indicated implantation superior efficacy promoting vascularized formation, enhancement synthesis development new blood vessels. our study indicate electrospun possess characteristics biomimetic periosteum, showing for effectively treating improving maintaining redox homeostasis BMMSCs.

Язык: Английский

ANXA2 promotes osteogenic differentiation and inhibits cellular senescence of periodontal ligament cells (PDLCs) in high glucose conditions DOI Creative Commons
Yanlin Huang, Jiaye Wang,

Chunhui Jiang

и другие.

PeerJ, Год журнала: 2024, Номер 12, С. e18064 - e18064

Опубликована: Сен. 18, 2024

Periodontal ligament cells (PDLCs) are a major component of the periodontal and have an important role in regeneration tissue maintenance homeostasis. High glucose can affect activity function PDLCs variety ways; therefore, it is particularly to find ways alleviate effects high on PDLCs. Annexin A2 (ANXA2) calcium- phospholipid-binding protein involved cellular functions processes, including cytokinesis, cytophagy, migration, proliferation.

Язык: Английский

Процитировано

0
The Role of Mitochondrial Permeability Transition in Bone Metabolism, Bone Healing, and Bone Diseases DOI Creative Commons

Xiting Zhu,

Zihao Qin, Min Zhou

и другие.

Biomolecules, Год журнала: 2024, Номер 14(10), С. 1318 - 1318

Опубликована: Окт. 17, 2024

Bone is a dynamic organ with an active metabolism and high sensitivity to mitochondrial dysfunction. The permeability transition pore (mPTP) low-selectivity channel situated in the inner membrane (IMM), permitting exchange of molecules up 1.5 kDa out IMM. Recent studies have highlighted critical role mPTP bone tissue, but there currently lack reviews concerning this topic. This review discusses structure function its impact on bone-related cells pathological states. activity reduced during osteogenic differentiation mesenchymal stem (MSCs), while desensitisation may underlie mechanism enhanced resistance apoptosis neoplastic osteoblastic cells. over-opening triggers swelling, regulated cell death, inflammatory response. In particular, involved dexamethasone-induced osteoblast dysfunction bisphosphonate-induced osteoclast apoptosis. vivo, plays significant maintaining homeostasis, many disorders linked excessive opening. Genetic deletion or pharmacological inhibition has shown potential enhancing injury recovery alleviating diseases. Here, we findings relationship at both cellular disease levels, highlighting novel avenues for approaches targeting promote healing manage disorders.

Язык: Английский

Процитировано

0
Irisin-loaded electrospun core-shell nanofibers as calvarial periosteum accelerate vascularized bone regeneration by activating the mitochondrial SIRT3 pathway DOI Creative Commons
Xi Hua,

Mingzhuang Hou,

Lei Deng

и другие.

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Июль 26, 2023

Abstract The scarcity of native periosteum poses a significant clinical barrier in the repair critical-sized bone defects. challenge enhancing regenerative potential healing is further compounded by oxidative stress at fracture site. However, introduction artificial has demonstrated its ability to promote regeneration through provision appropriate mechanical support and controlled release pro-osteogenic factors. In this study, polylactic acid (PLLA)/hyaluronic (HA)-based nanofibrous membrane was fabricated using coaxial electrospinning technique. incorporation irisin into core-shell structure PLLA/HA nanofibers (PLLA/HA@Irisin) achieved sustained release. vitro experiments that PLLA/HA@Irisin membranes exhibited favorable biocompatibility. osteogenic differentiation marrow mesenchymal stem cells (BMMSCs) improved PLLA/HA@Irisin, as evidenced increase alkaline phosphatase (ALP) activity matrix mineralization. Mechanistically, significantly enhanced mitochondrial function BMMSCs via activation sirtuin 3 antioxidant pathway. To assess therapeutic effectiveness, were implanted situ calvarial defects rats. results four eight weeks post-surgery indicated implantation superior efficacy promoting vascularized formation, enhancement synthesis development new blood vessels. our study indicate electrospun possess characteristics biomimetic periosteum, showing for effectively treating improving maintaining redox homeostasis BMMSCs.

Язык: Английский

Процитировано

0