Increased calcification by erythrophagocytosis in aortic valvular interstitial cells DOI Creative Commons

Zihan Qin,

Magnus Bäck, Anders Franco‐Cereceda

и другие.

ESC Heart Failure, Год журнала: 2024, Номер unknown

Опубликована: Окт. 27, 2024

Abstract Background Calcific aortic valve disease (CAVD) progresses over time to severe stenosis and eventually heart failure. Recent evidence indicates that intraleaflet haemorrhage (ILH) strongly promotes CAVD progression. However, it remains poorly understood how mechanistically contributes valvular calcification. Method ILH was identified as iron deposition by morphological analysis. To elucidate the underlying mechanism, human interstitial cells (VIC) were cultured in presence of fresh or senescent red blood (RBC), simulating vivo conditions. Result common valves derived from patients with stenosis. VIC undergo erythrophagocytosis RBC, leading intracellular accumulation analogous observed following exposure extracellular iron. The RBC significantly intensified calcification, which mitigated ferroptosis inhibition. Conclusions Our results identify VIC, enhanced calcification through ferroptosis. This may be a crucial component pathophysiological mechanisms links accelerated

Язык: Английский

Research progress on ferroptosis in Myelodysplastic syndromes DOI Creative Commons

Yifan Yang,

Jiongping Han,

Yanjun Wei

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Фев. 28, 2025

Myelodysplastic syndromes (MDS) are a group of malignancies characterized by clonal proliferation hematopoietic stem cells, ineffective hematopoiesis, peripheral cytopenias, and high risk transformation to acute myeloid leukemia. Current therapeutic strategies for MDS have limited efficacy. Thus, identifying new targets prognostic biomarkers is critical future research direction. Ferroptosis, type iron-dependent programmed cell death, has become recent hotspot in the field oncology research. Recent results demonstrated that iron metabolism, lipid other pathways can be targeted induce ferroptosis cells. In addition, ferroptosis-related genes significance prognosis diagnosis MDS. This article reviews current progress on MDS, including its potential targeting as intervention strategy.

Язык: Английский

Процитировано

0

Nesfatin-1 enhances vascular smooth muscle calcification through facilitating BMP-2 osteogenic signaling DOI Creative Commons
Xuexue Zhu, Xinyu Meng, Guo Chen

и другие.

Cell Communication and Signaling, Год журнала: 2024, Номер 22(1)

Опубликована: Окт. 11, 2024

Vascular calcification (VC) arises from the accumulation of calcium salts in intimal or tunica media layer aorta, contributing to higher risk cardiovascular events and mortality. Despite this, mechanisms driving VC remain incompletely understood. We previously described that nesfatin-1 functioned as a switch for vascular smooth muscle cells (VSMCs) plasticity hypertension neointimal hyperplasia. In this study, we sought investigate role mechanism VC. The expression was measured calcified VSMCs aortas, well patients. Loss- gain-of-function experiments were evaluated roles pathogenesis. transcription activation detected using mass spectrometry. found levels both patients with coronary calcification. Loss-of-function revealed key regulator by facilitating osteogenic transformation VSMCs. Mechanistically, promoted de-ubiquitination stability BMP-2 via inhibiting E3 ligase SYTL4, interaction potentiated signaling induced phosphorylation Smad, followed HDAC4 nuclear exclusion. dissociation RUNX2 elicited acetylation subsequent translocation, leading upregulation OPN, critical player From small library natural compounds, identified Curculigoside Chebulagic acid reduced development binding nesfatin-1. Eventually, designed spectrometry-based DNA-protein screening identify STAT3 mediated context Overall, our study demonstrates enhances thereby stabilizing downstream SMAD1/5/9 HDAC4. This cascade leads transcriptional MSX2, These insights position potential therapeutic target preventing treating VC, advancing understanding molecular underlying condition.

Язык: Английский

Процитировано

1

Increased calcification by erythrophagocytosis in aortic valvular interstitial cells DOI Creative Commons

Zihan Qin,

Magnus Bäck, Anders Franco‐Cereceda

и другие.

ESC Heart Failure, Год журнала: 2024, Номер unknown

Опубликована: Окт. 27, 2024

Abstract Background Calcific aortic valve disease (CAVD) progresses over time to severe stenosis and eventually heart failure. Recent evidence indicates that intraleaflet haemorrhage (ILH) strongly promotes CAVD progression. However, it remains poorly understood how mechanistically contributes valvular calcification. Method ILH was identified as iron deposition by morphological analysis. To elucidate the underlying mechanism, human interstitial cells (VIC) were cultured in presence of fresh or senescent red blood (RBC), simulating vivo conditions. Result common valves derived from patients with stenosis. VIC undergo erythrophagocytosis RBC, leading intracellular accumulation analogous observed following exposure extracellular iron. The RBC significantly intensified calcification, which mitigated ferroptosis inhibition. Conclusions Our results identify VIC, enhanced calcification through ferroptosis. This may be a crucial component pathophysiological mechanisms links accelerated

Язык: Английский

Процитировано

0