Journal of Fish Diseases,
Год журнала:
2024,
Номер
47(11)
Опубликована: Июль 29, 2024
Abstract
Globally,
streptococcal
disease
caused
by
Streptococcus
agalactiae
is
known
for
its
high
mortality
rate,
which
severely
limits
the
development
of
tilapia
breeding
industry.
As
a
third‐generation
vaccine,
DNA
vaccines
have
shown
great
application
prospects
in
prevention
and
control
aquatic
diseases,
but
their
low
immunogenicity
development.
The
combination
molecular
adjuvants
proved
to
be
an
effective
method
inducing
protective
immunity.
This
study
constructed
recombinant
plasmids
encoding
HSP70
IL‐1β
genes
(pcHSP70
pcIL‐1β)
verify
effectiveness
as
S.
vaccine
(pcSIP)
immunized
model.
results
revealed
that
serum‐specific
IgM
production,
enzyme
activities,
immune‐related
gene
expression
with
pcSIP
plus
pcHSP70
or
pcIL‐1β
were
significantly
higher
than
those
alone.
It
worth
noting
improved
immune
protection
vaccines,
relative
percentage
survival
(RPS)
51.72%
(pcSIP
pcHSP70)
44.83%
pcIL‐1β),
respectively,
compared
alone
(24.14%).
Thus,
our
indicated
are
promising
controlling
infection.
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Авг. 12, 2024
Biomaterials
are
substances
that
can
be
injected,
implanted,
or
applied
to
the
surface
of
tissues
in
biomedical
applications
and
have
ability
interact
with
biological
systems
initiate
therapeutic
responses.
Biomaterial-based
vaccine
delivery
possess
robust
packaging
capabilities,
enabling
sustained
localized
drug
release
at
target
site.
Throughout
process,
they
contribute
protecting,
stabilizing,
guiding
immunogen
while
also
serving
as
adjuvants
enhance
efficacy.
In
this
article,
we
provide
a
comprehensive
review
contributions
biomaterials
advancement
development.
We
begin
by
categorizing
biomaterial
types
properties,
detailing
their
reprocessing
strategies,
exploring
several
common
systems,
such
polymeric
nanoparticles,
lipid
hydrogels,
microneedles.
Additionally,
investigated
how
physicochemical
properties
routes
influence
immune
Notably,
delve
into
design
considerations
adjuvants,
showcasing
application
development
for
cancer,
acquired
immunodeficiency
syndrome,
influenza,
corona
virus
disease
2019
(COVID-19),
tuberculosis,
malaria,
hepatitis
B.
review,
highlight
successful
instances
where
enhanced
efficacy
discuss
limitations
future
directions
immunotherapy.
This
aims
offer
researchers
understanding
stimulate
further
progress
related
fields.
Aquaculture Reports,
Год журнала:
2023,
Номер
32, С. 101739 - 101739
Опубликована: Сен. 18, 2023
To
encourage
aquaculture
sustainability
and
lower
fish
production
costs,
products
need
to
be
substituted
with
sustainable
ingredients
in
aquafeeds.
Toward
this
goal,
we
examined
whether
a
natural
mix
of
highly
concentrated
free
amino
acids
could
alleviate
the
negative
effects
low–fish
meal
(FM)
diets
on
Nile
tilapia
terms
growth
performance
nutrient
utilization.
In
present
study,
2
×
3
factorial
design
two
FM
levels
(5
%
10
%)
was
used
prepare
experimental
three
Kera-Stim®
50
(KS),
acid
mixture,
supplemented
(0
%,
0.25
0.5
%).
(average
initial
body
weight
20
g)
were
cultured
18
cages
provided
one
six
for
eight
weeks.
We
found
that
dietary
KS
supplementation
significantly
increased
parameters
including
final
weight,
gain,
specific
rate
compared
without
(P
<
0.05).
Moreover,
had
substantial
positive
effect
feed
conversion
ratio
Interestingly,
not
only
low-FM
but
also
economic
profit
index,
suggesting
efficiency.
Furthermore,
lysozyme,
catalase,
superoxide
dismutase,
glutathione
peroxidase,
reductase
activity
by
supplementing
whole-body
ash,
fat,
crude
protein
polyunsaturated
fatty
acids,
such
as
arachidonic
(20:4
n6)
docosahexaenoic
(C22:6
n3).
concluded
diet
can
mitigate
nutritional
achieve
optimum
compromising
or
revenue-to-cost
ratios.
E3S Web of Conferences,
Год журнала:
2023,
Номер
442, С. 02010 - 02010
Опубликована: Янв. 1, 2023
Bacterial
vaccines
have
been
tested
for
their
effectiveness
in
protecting
fish
from
potential
virulent
diseases.
The
current
vaccine
preparations
are
mostly
liquid
form,
which
affects
prices
due
to
an
increase
the
cost
of
shipment,
stability
products
also
has
limitations
long-term
storage.
This
study
aims
develop
a
method
preparation
bivalent
through
freeze
dry
with
skimmed
milk
as
filler
optimization
products.
by
can
reduce
96.67%
total
weight
compared
form.
quality
test
showed
that
containing
bacteria
Aeromonas
hydrophila
and
Streptococcus
agalactiae
passed
sterility
viability
test.
application
provide
positive
response
antibody
titer
other
hematological
parameter
responses
survival
rate
fish.
development
freeze-dried
optimize
recommended
product
disease
management.
