Gene mobility elements mediate cell type specific genome organization and radial gene movement in vivo DOI Creative Commons
Tanguy Lucas, Lin-Ing Wang, Juniper Glass-Klaiber

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 1, 2024

ABSTRACT Understanding the level of genome organization that governs gene regulation remains a challenge despite advancements in chromatin profiling techniques. Cell type specific architectures may be obscured by averaging heterogeneous cell populations. Here we took reductionist perspective, starting with relocation hunchback to nuclear lamina Drosophila neuroblasts. We previously found this event terminates competence produce early-born neurons and is mediated an intronic 250 base-pair element, which term mobility element (GME). over 800 putative GMEs globally are accessible Polycomb (PcG) target sites. appear distinct from PcG response elements, however, largely inaccessible Performing situ Hi-C purified neuroblasts, form megabase-scale interactions, spanning multiple topologically associated domain borders, preferentially contacting other GMEs. These interactions stage-specific. Notably, undergo developmentally- timed mobilization to/from neuroblast lamina, swapping GFP reporter transgene intron GME relocates embryos. propose constitute organizational framework mediate gene-to-lamina during progenitor state transitions vivo .

Язык: Английский

Gene mobility elements mediate cell type specific genome organization and radial gene movement in vivo DOI Creative Commons
Tanguy Lucas, Lin-Ing Wang, Juniper Glass-Klaiber

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 1, 2024

ABSTRACT Understanding the level of genome organization that governs gene regulation remains a challenge despite advancements in chromatin profiling techniques. Cell type specific architectures may be obscured by averaging heterogeneous cell populations. Here we took reductionist perspective, starting with relocation hunchback to nuclear lamina Drosophila neuroblasts. We previously found this event terminates competence produce early-born neurons and is mediated an intronic 250 base-pair element, which term mobility element (GME). over 800 putative GMEs globally are accessible Polycomb (PcG) target sites. appear distinct from PcG response elements, however, largely inaccessible Performing situ Hi-C purified neuroblasts, form megabase-scale interactions, spanning multiple topologically associated domain borders, preferentially contacting other GMEs. These interactions stage-specific. Notably, undergo developmentally- timed mobilization to/from neuroblast lamina, swapping GFP reporter transgene intron GME relocates embryos. propose constitute organizational framework mediate gene-to-lamina during progenitor state transitions vivo .

Язык: Английский

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