Scientific Reports, Год журнала: 2024, Номер 14(1)
Опубликована: Дек. 19, 2024
Язык: Английский
Nature Reviews Genetics, Год журнала: 2025, Номер unknown
Опубликована: Апрель 3, 2025
Язык: Английский
Процитировано
1
Genome Research, Год журнала: 2023, Номер 33(12), С. 2079 - 2093
Опубликована: Дек. 1, 2023
Mammalian sperm show an unusual and heavily compacted genomic packaging state. In addition to its role in organizing the compact hydrodynamic head, it has been proposed that chromatin architecture helps program gene expression early embryo. Scores of genome-wide surveys have reported patterns accessibility, nucleosome localization, histone modification, chromosome folding. Here, we revisit these studies light recent reports obtained from mouse epididymis are contaminated with low levels cell-free chromatin. absence proper lysis, readily recapitulate multiple prominent chromatin, suggesting profiles primarily reflect contaminating Removal DNA, appropriate lysis conditions, together required reveal a state distinct most previous reports. Using ATAC-seq explore relatively accessible loci, identify landscape open loci associated development transcriptional control. Histone modification folding also strongly support hypothesis prior suffer contamination, but technical challenges reliably preserving head prevent us confidently assaying true localization for epigenetic marks. Together, our knowledge mammalian remains largely incomplete, motivate future efforts more accurately characterize genome organization mature sperm.
Язык: Английский
Процитировано
19
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(2), С. 718 - 718
Опубликована: Янв. 5, 2024
Infertility affects millions of couples worldwide and has a profound impact not only on their families, but also communities. Telomere attrition been associated with infertility, DNA damage fragmentation. Oxidative stress shown to affect sperm integrity telomere length. Sirtuins such as SIRT1 SIRT3 are involved in aging oxidative response. The aim the present study is determine role regulating stress, shortening, association oligospermia. Therefore, we assessed protein levels SIRT3, total antioxidant capacity (TAC), superoxide dismutase (SOD), malondialdehyde (MDA) catalase activity (CAT) seminal plasma 272 patients oligospermia 251 fertile men. We measured length (STL) leukocyte (LTL) using standard real-time quantitative PCR assay. Sperm chromatin protamine deficiency were per methods. Our results for oligospermic demonstrate significant reductions semen parameters, shorter STL LTL, lower SOD, TAC, CAT, levels, higher MDA conclude that TL sperms leukocytes increased accounts high fragmentation sperms. support hypothesis various parameters state or caused by reduced proteins.
Язык: Английский
Процитировано
8
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Фев. 8, 2024
ABSTRACT DNA methylation (DNAme) serves a stable gene regulatory function in somatic cells (1). In the germ line and during early embryogenesis, however, DNAme undergoes global erasure re-establishment to support cell embryonic development (2). While de novo acquisition male is essential for setting genomic imprints, other intergenerational roles paternal defining chromatin after fertilization are unknown. To approach this question, we reduced levels of developing through conditional deletion methyltransferases DNMT3A DNMT3B undifferentiated spermatogonia. We observed that maintenance spermatogonia while catalyzes spermatogonial differentiation. Mutant nevertheless completed their differentiation sperm. Failing Dnmt3a / Dnmt3b double deficient associated with increased nucleosome occupancy mature sperm, preferentially at sites higher CpG content, supporting model modulates retention sperm (3). assess impact altered formation chromatin, measured H3K4me3 maternal alleles 2-cell embryos using newly developed transposon-based tagging assay modified chromatin. Our data show renders permissive establishment embryos, independently possible inheritance born H3K4me3. Together, study provides first evidence paternally inherited directs development.
Язык: Английский
Процитировано
4
Current Opinion in Genetics & Development, Год журнала: 2024, Номер 86, С. 102190 - 102190
Опубликована: Апрель 11, 2024
Язык: Английский
Процитировано
4
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown
Опубликована: Янв. 11, 2025
Abstract DNA methylation patterns are inherited from the parental germline to embryo. In mature sperm, sites of unmethylated tightly coupled histone retention at gene regulatory elements that implicated in paternal epigenetic inheritance. The timing and mechanism site-specific demethylation male currently remains unknown. Here, we perform genome-wide profiling during spermatogenesis by capturing methylated through interaction with a methyl-DNA binding protein domain (MBD). Our data demonstrate there is change mitosis-to-meiosis transition. Importantly, genomic demethylated this transition predetermine nucleosome spermatozoa. These results suggest prepares embryonic expression after fertilization. We therefore propose as novel phase reprogramming contributes regulation.
Язык: Английский
Процитировано
0
Aging Cell, Год журнала: 2024, Номер 23(8)
Опубликована: Май 16, 2024
Abstract The sperm epigenome is thought to affect the developmental programming of resulting embryo, influencing health and disease in later life. Age‐related methylation changes old fathers may mediate increased risks for reproductive offspring medical problems. impact paternal age on has been extensively studied humans and, a lesser extent, rodents cattle. Here, we performed comparative analysis effects protein‐coding genes human marmoset methylomes. gained growing importance as non‐human primate model aging age‐related diseases. Using reduced representation bisulfite sequencing, identified differentially methylated transcription start site (ageTSS) regions 204 27 genes. direction was opposite, increasing with marmosets decreasing humans. None ageTSS both species. Although average levels all TSS were highly correlated between humans, majority being hypomethylated sperm, more than 300 endowed species‐specifically (hypo)methylated TSS. Several glycosphingolipid (GSL) biosynthesis pathway, which plays role embryonic stem cell differentiation regulation development, (<5%) fully (>95%) sperm. expression patterns defined sets GSL differed considerably pre‐implantation embryo stages blastocyst tissues, respectively.
Язык: Английский
Процитировано
3
Nature Communications, Год журнала: 2025, Номер 16(1)
Опубликована: Янв. 7, 2025
Abstract In the germ line and during early embryogenesis, DNA methylation (DNAme) undergoes global erasure re-establishment to support cell embryonic development. While DNAme acquisition male development is essential for setting genomic imprints, other intergenerational roles paternal in defining chromatin are unknown. Through conditional gene deletion of de novo methyltransferases Dnmt3a and/or Dnmt3b , we observe that DNMT3A primarily safeguards against hypomethylation undifferentiated spermatogonia, while DNMT3B catalyzes spermatogonial differentiation. Failing / double deficient spermatogonia associated with increased nucleosome occupancy mature sperm, preferentially at sites higher CpG content, supporting model modulates retention sperm. To assess impact altered sperm formatting chromatin, measure H3K4me3 maternal alleles 2-cell embryos using a transposon-based tagging approach. Our data show reduced renders permissive establishment embryos, independently possible inheritance born H3K4me3. Together, this study provides evidence paternally inherited directs formation
Язык: Английский
Процитировано
0
EMBO Molecular Medicine, Год журнала: 2025, Номер unknown
Опубликована: Янв. 22, 2025
Abstract Recent studies argue for a novel concept of the role chromatin as carrier epigenetic memory through cellular and organismal generations, defining coordinating gene activity states physiological functions. Environmental insults, such exposures to unhealthy diets, smoking, toxic compounds, infections, can epigenetically reprogram germ-line cells influence offspring phenotypes. This review focuses on intergenerational transgenerational inheritance in different plants, animal species humans, presenting up-to-date evidence arguments effects light Darwinian Lamarckian evolutionary theories. An overview changes induced by infection or other immune challenges is presented, how these changes, known epimutations, contribute shaping The mechanisms that mediate transmission alterations via germline are also discussed. Understanding relationship between environmental fluctuations, resistance, susceptibility diseases critical unraveling disease etiology adaptive evolution.
Язык: Английский
Процитировано
0
Опубликована: Апрель 9, 2025
DNA methylation patterns are inherited from the parental germline to embryo. In mature sperm, sites of unmethylated tightly coupled histone retention at gene regulatory elements that implicated in paternal epigenetic inheritance. The timing and mechanism site-specific demethylation male currently remains unknown. Here, we perform genome-wide profiling during spermatogenesis by capturing methylated through interaction with a methyl-DNA binding protein domain (MBD). Our data demonstrate there is change mitosis-to-meiosis transition. Importantly, genomic demethylated this transition predetermine nucleosome spermatozoa. These results suggest prepares embryonic expression after fertilization. We therefore propose as novel phase reprogramming contributes regulation.
Язык: Английский
Процитировано
0