Soil labile organic carbon fractions mediate microbial community assembly processes during long‐term vegetation succession in a semiarid region

iMeta, Год журнала: 2023, Номер 2(4)

Опубликована: Окт. 22, 2023

Conceptual diagram for the labile organic carbon (OC) fractions mediating microbial assembly processes during long-term vegetation succession. Owing to its sparse and low soil (SOC) content, Loess Plateau is one of regions with highest (C) sequestration potential [1]. Vegetation succession a widely adopted strategy ecosystem recovery capacity rehabilitate degraded lands facilitate matter in semiarid region [2-5]. Additionally, it can influence underground community dynamics [6, 7]. The Ziwuling has undergone approximately 160 years secondary succession, making unique area complete sequence natural following farmland abandonment on [4]. Thus, this provide us better understanding patterns changes (Figure 1A Supporting Information: Table S1). Considering vital role microorganisms function, examining temporal communities crucial [8]. composition are essential function [9, 10]. Deterministic stochastic two primary ecological involved [11]. Traditionally, been regarded as being primarily influenced by deterministic processes, aligning principle that, "everything everywhere, but environment selects" [12]. Nevertheless, should not be disregarded when considering random extinctions or dispersal events [13]. Land use type also notable effect [14-16]. Stochastic have greater agricultural fields, whereas their impact diminishes forested soils, bacterial temperate grasslands [16]. Furthermore, there environmental differences between topsoil (0–20 cm) subsoil (20–40 terrestrial ecosystems [17]. Previous studies indicated that dominate assembly, drift prevailing limitation playing dominant subtropical paddy soils [18]. This indicates associations characteristics different spatial distributions, which may affect function. Although extensive research conducted process [12, 19, 20], uncertainties about remain owing resource balance strongly abiotic factors including nitrogen [19, 21, 22]. Research salt marsh plant shown fluctuations SOC exert strongest selective pressure assemblies [23]. Among eight indicators, had greatest [24]. commonly conceptualized stable C fraction storage models [25], easily oxidizable (EOC), particulate (POC), dissolved considered OC serve early indicators variation [26, 27]. In contrast, recalcitrant (ROC) mineral-associated highly [28]. functional diversity SOC, many these predominantly focused association 29, 30], leaving relationship relatively unclear. Within framework biotic cannot overlooked, could determine attributes niche occupancy 31]. Keystone taxa connected taxonomic groups play roles functionality [32]. For example, Sulfuricella, Rhodobacter, Comamonadaceae, drive mediated graphene derivatives [33]. However, keystone largely unknown ecosystems, enhancing comprehending functionality. To address issues, were selected progression explore structure, co-occurrence networks, relationships fractions. We hypothesized that: (1) stronger than because nutrient limitations, (2) given represents readily usable energy source microorganisms, likely mainly governed Long-term significantly increased accumulation 0–40 cm from pioneer weed stage onwards (p < 0.001; Figure 1B). content was higher all stages After shrubland stage, compared When reached forest (>110 years), gradually stabilized Succession age depth affected comprised phyla Proteobacteria, Actinobacteria, Acidobacteria, Gemmatimonadota, Chloroflexi 1C). Basidiomycota, Ascomycota, Mortierellomycota, Glomeromycota, Kixellomycota fungal abundances Actinobacterta, Mortierellomycota subsoil, those Chloroflexi, Ascomycota lower 0.05; relative Overall, decreased alpha communities, Chao 1 Shannon indices (Supporting S2). A combination NMDS analysis, ANOSIM, Adonis statistical tests varied 1D). Soil Tables S2 S3). along across depths investigated using null model analyses. results showed (|βNTI| > 2), especially homogeneous selection, critical bacteria fungi at both 2A–D). weaker subsoil. (homogeneous selection) later 2A,C). Drift limitations 2B,D). networks distinct each 2E–H). Edges average degree depth, demonstrating network more complex (Table S4). positive correlations cross-kingdom further explored S3), found nodes edges among climax forests S4), suggesting complexity networks. Network analysis identified genus Dongia (ASV901) amplicon variant (ASV) bacteria, genera MND1 (ASV155) MB-A2-108 (ASV266) 2E,F S5). ASVs included (ASV4), family Pezizaceae (ASV45), Fusarium (ASV136), class Sordarioclamycetes (ASV689) 2G Alternaria (ASV44), (ASV70), Filobasidium (ASV374), order Chaetothyriales (ASV250) 2H abundance negatively correlated 3A). It worth noting ASV901 correlation taxon–environment community. consisted 60 230 3B), while 44 104 3B). EOC (connection number: 42) most important closely associated EOC, POC, ROC (with connection numbers 20, respectively) linear regression reflected variations bacteria. providing suitable explanation Figures S5 S6). These demonstrated effectively explained OC. Therefore, used mediators illustrate impacts partial least squares path (PLS-PM) 3C–F). main drivers regulated affecting traits total (0.51) S7A), (−0.56) S7C). Bacterial impacted 3D,F S12). effects (0.73) (−0.97) respectively S7B,D). Microbial various life strategies generate responses, [34]. Based mineralization growth rates, classified into r- (copiotrophic, fast-growing) K-strategists (oligotrophic, slow-growing) [35]. study, shifted S8), agreement previous [36, 37]. increase ratio extracellular enzyme activity corroborates result S9 S10). produce enzymes degrade [38-40]. An unstable conducive rapid r-strategists [40], driven [39]. species reduce availability [41]. indicate community, shifts K-strategists, strengthens stability. resources, resulting ASV [42, 43]. Ecological comprising ratios negative due interactions decreasing oscillations disturbed [44, 45]. stability fungi. modularity revealed limiting losing [46]. 2E,H suggests depths. fewer perturbations maintain number nodes, edges, correlations, indicating tended stabilize stage. other generally pools [47]. Consequently, factor communities. suggest increases Clarifying [48]. dominated 2A−D). niche-based theory states structure [14]. Stronger extreme, low-resource general phenomenon [13], explains (lower βNTI) supports first hypothesis. Homogeneous selection usually determined similar conditions exerted significant forces, less expected happen chance [49]. despite still dominating consistent current views, advancement supply under weak Extreme pH acts strict filter phylogenetic clustering, regardless successional age, [50, 51]. we difference small S11), thought caused slow shift processes. importance suddenly 2A,B), related nutrients such PLS-PM driving mechanisms 3C–F S7), confirmed second an applied affects [26]. controlling they [42]. study suggested paramount increase, diminishes. βNTI supported observation pathogenic rhizosphere, decrease benefits maintenance rhizosphere [52, 53]. recognized beneficial bacterium aid pathogen resistance, Xanthomonadaceae, ensure healthy [54]. assembly. Interactions common facilitating 55]. 3C–F), conclusion attributed Some volatiles released antagonistic [56], decomposition [57]. summary, OC, regulating thereby process. illustrated fractions, Given key rather sequences. neutral hypothesis [23], highlighting non-negligible determining directly tested laboratory, so validation through controlled experiments required confirm relationship. new targeted therapy indicator microenvironment. examined cultured metagenomic metatranscriptomic approaches future studies. show drove taxa. addition, Taken together, provides insights link over long scales. Jingwei Shi Lin Yang conceived supervised study. Jiwei Li established experimental sites. Liao collected samples analyzed data. Shuo Jiao, Zhouping Shangguan, Lei Deng assisted data analysis. wrote manuscript input authors. National Natural Science Foundation China (42277471, 42307578, U2243225), Key Development Program Shaanxi Province (2021ZDLSF05-02), Funding Top Young Talents Ten Thousand Plan (2021) Forestry Grassland Administration (2020132601). authors declare no conflict interest. support findings available corresponding author upon reasonable request. DNA sequences generated Center Biotechnology Information's Gen-Bank database project accession PRJNA1022789 PRJNA1023009. scripts saved GitHub https://github.com/Shijingweisjw/iMETA-R-code. Materials (figures, tables, scripts, graphical abstract, slides, videos, Chinese translated version, updated materials) online DOI iMeta http://www.imeta.science/ Please note: publisher responsible any supporting information supplied Any queries (other missing content) directed article.

Язык: Английский

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High stochasticity in rare bacterial community assembly in rice-wheat rotation soils at a regional scale

Soil Biology and Biochemistry, Год журнала: 2024, Номер 195, С. 109479 - 109479

Опубликована: Май 20, 2024

Язык: Английский

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Impacts of forest expansion on microbial diversity and community assembly in fragmented mountain ecosystems

Environmental Research, Год журнала: 2025, Номер unknown, С. 120956 - 120956

Опубликована: Янв. 1, 2025

Язык: Английский

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Effect of aridity on the β-diversity of alpine soil potential diazotrophs: insights into community assembly and co-occurrence patterns

mSystems, Год журнала: 2023, Номер 9(1)

Опубликована: Дек. 7, 2023

Microbial diversity plays a vital role in the maintenance of ecosystem functions. However, current understanding mechanisms that shape microbial along environmental gradients at broad spatial scales is relatively limited, especially for specific functional groups, such as potential diazotrophs. Here, we conducted an aridity-gradient transect survey from 60 sites across Tibetan Plateau, largest alpine planet, to investigate ecological processes (e.g., local species pools, community assembly processes, and co-occurrence patterns) underlie β-diversity soil diazotrophic communities. We found aridity strongly negatively affected abundance, richness, Diazotrophs displayed distance-decay pattern gradient, with organisms living lower habitats having stronger pattern. Arid had complexity, including number edges vertices, average degree, keystone taxa, compared humid habitats. Local pools explained limited variations β-diversity. In contrast, patterns stochastic dispersal limitation drift) played significant regulating The relative importance changed increasing aridity, weakening whereas enhancing. genera

Язык: Английский

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Bacterial and Fungal Communities Respond Differently to Changing Soil Properties Along Afforestation Dynamic

Microbial Ecology, Год журнала: 2025, Номер 88(1)

Опубликована: Фев. 6, 2025

Abstract Spontaneous afforestation following land abandonment has been increasingly recognized as a nature-based solution to mitigate climate change and provide measurable benefits biodiversity. However, effects on biodiversity, particularly soil microbial communities, are still poorly characterized, with most previous studies focusing artificial plantations rather than forest rewilding dynamics. Here, we assessed changes in topsoil physical–chemical properties related dynamics of bacterial fungal community composition structure spontaneous abandoned grasslands Northeast Italy over the last 70 years. With space-for-time approach, selected four chronosequences representing different successional stages: grassland, early (2000–2020), intermediate (1978–2000), late (1954–1978). Results showed that progressively reduced pH total phosphorus (P), while organic carbon (SOC), nitrogen (N), C:N ratio increased. Correspondingly, overall α-diversity community, by ITS DNA metabarcoding, decreased after an initial increase from grassland conditions, substrate acidification trophic specialization. Bacterial diversity, 16S was highest at stages, then later likely limited lower matter quality. Shifts included ectomycorrhizal Basidiomycota linked topsoil’s higher SOC, N, ratio. Differently, responded substantially pH, acidity favoring Proteobacteria (Pseudomonadota) Acidobacteria (Acidobacteriota) stages. Our findings first contribution clarify how fungi bacteria respond afforestation. This is relevant context mitigation, considering fundamental role microorganisms shaping storage

Язык: Английский

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Reforestation significantly enriches soil microbial carbon, nitrogen, and phosphorus cycling genes but simplifies their co-occurrence network

Applied Soil Ecology, Год журнала: 2025, Номер 207, С. 105935 - 105935

Опубликована: Фев. 9, 2025

Язык: Английский

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Effects of cropland abandonment succession on soil microorganism and multifunctionality in the arid zone of Northwest China

Applied Soil Ecology, Год журнала: 2025, Номер 207, С. 105954 - 105954

Опубликована: Фев. 10, 2025

Язык: Английский

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Mixed eucalyptus plantations in southern subtropical China effectively stabilized the rhizosphere-associated bacterial network and facilitated soil ecological function

Applied Soil Ecology, Год журнала: 2025, Номер 208, С. 105963 - 105963

Опубликована: Фев. 19, 2025

Язык: Английский

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Stochastic processes dominate bacterial and fungal community assembly in ultra-high-altitude areas of southeast Tibet

Ecological Indicators, Год журнала: 2025, Номер 173, С. 113394 - 113394

Опубликована: Март 29, 2025

Язык: Английский

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Unraveling microbial assembly and co-occurrence relationship at seasonal and vertical scales in an aged organic-contaminated site

International Biodeterioration & Biodegradation, Год журнала: 2025, Номер 202, С. 106080 - 106080

Опубликована: Апрель 4, 2025

Язык: Английский

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