Arsenic
(As),
found
in
diverse
ecosystems,
poses
major
public
health
risks
various
parts
of
the
world.
Arsenotrophic
bacteria
contaminated
environments
help
reduce
toxicity
by
converting
arsenite
(AsIII)
to
less
harmful
arsenate
(AsV).
We
assumed
that
Achromobacter
aegrifaciens
strains
from
As-contaminated
tubewell
water
and
soil
would
share
similar
genomic
characteristics
associated
with
arsenic
detoxification
bioremediation.
To
investigate
this,
we
employed
both
culture-dependent
culture-independent
viz.
whole
genome
sequencing
(WGS)
methods
thoroughly
elucidate
phenotypic
genotypic
features
two
A.
isolated
(BAW48)
(BAS32)
samples
collected
Bogura
district
Bangladesh.
Both
BAW48
BAS32
isolates
demonstrated
As(III)
oxidation
KMNO4
test,
which
was
corroborated
molecular
analysis
confirming
presence
aioA
arsB
genes
strains.
These
were
be
phylogenetically
related
many
spp.,
biological
inorganic
reactors,
environmental
soils,
sediments
human
clinical
across
geographical
regions.
Moreover,
possessed
distinct
heavy
metal
resistance
conferring
Co,
Zn,
Cu,
Cd,
Hg,
As,
Cr.
Three
As
gene
clusters
such
as
oxidizing
aioBA,
reducing
arsRCDAB
MMA(III)
ars
(arsHCsO)
cluster
predicted
genomes
aegrifaciens.
Further
analyses
revealed
profiles
strains,
mobile
genetic
elements,
antimicrobials
genes,
virulence
metabolic
features.
Pangenome
synteny
showed
are
evolutionary
other
but
closely
one
another.
The
data
confirmed
can
oxidize
detoxify
metals
like
suggesting
their
potential
for
findings
align
our
assumption
provide
a
basis
developing
sustainable
solutions
bioremediation
efforts
environments.
Antibiotics,
Год журнала:
2023,
Номер
12(12), С. 1678 - 1678
Опубликована: Ноя. 29, 2023
The
study
examines
the
antibiotic
resistance
of
metal-tolerant
bacteria
isolated
from
wastewater
treatment
plant
a
large
city
to
six
antibiotics
belonging
β-lactam
antibiotics,
aminoglycosides
and
amphenicols.
Resistance
sewage
sludge
multitolerant
heavy
metals
18
tetracyclines,
aminoglycosides,
diaminopyrimidines,
amphenicols
ansamycins
was
studied
also.
Out
10,
microorganisms
facilities
only
Klebsiella
pneumonia
strain
(tolerant
3
mM
Cu)
secondary
settling
tank
did
not
show
at
concentrations
considered.
maximum
amount
typical
for
strains
Serratia
fonticola
SS0-1,
fresh
resistant
5
mmol
Cu
Pb,
or
Stenotrophomonas
maltophilia
SS0-5,
also
Zn
Cu.
It
is
possible
that
bacterial
develops
as
result
use
themselves,
but
environmental
pollution
with
metals,
vice
versa.
Asian Journal of Chemistry,
Год журнала:
2024,
Номер
36(3), С. 531 - 542
Опубликована: Фев. 28, 2024
The
release
of
metals
into
the
environment
raises
serious
concerns
about
their
harmful
effects
on
both
wildlife
and
human
health.
biosphere
is
experiencing
with
pervasive
presence
heavy
metal
pollutants
such
as
arsenic
(As),
cadmium
(Cd),
mercury
(Hg),
lead
(Pb),
chromium
(Cr),
copper
(Cu)
nickel
(Ni),
which
pose
significant
environmental
challenges.
While
certain
are
essential
for
regulating
fundamental
metabolic
processes
upholding
overall
physiology
microorganisms,
excessive
exposure
to
can
be
detrimental
survival
function.
As
a
result
remarkable
adaptability,
particularly
bacteria
Pseudomonas
fluorescens,
Escherichia
coli,
Serratia
marcescens,
Bacillus
cereus
Alcaligenes
sp.,
have
evolved
sophisticated
defence
mechanisms
combat
stress
caused
by
metals.
One
process
creation
metal-binding
proteins
(MBPs),
may
bind
sequester
metals,
thus
significantly
lowering
toxicity
in
bacteria.
Metalloproteomics,
subfield
metallomics,
focuses
discovery
characterization
(MBPs)
metal-resistant
bacteria,
resulting
opening
doors
innovative
bioremediation
techniques
therapeutic
treatments
against
bacterial
diseases.
This
review
explores
intriguing
world
MBPs
emphasizes
role
resistance,
detoxification
homeostasis.
Furthermore,
metallochaperones
been
extensively
studied
using
metalloproteomic
methodologies
utilized
proteins.
study
also
provides
useful
information
interactions
between
these
different
MBPs,
advances
our
understanding
how
respond
Bioremediation Journal,
Год журнала:
2024,
Номер
unknown, С. 1 - 14
Опубликована: Март 14, 2024
Cr(VI)
is
one
of
the
most
widely
used
elements
in
various
industrial
sectors
and,
hence,
extensively
found
as
a
toxicant
ecosystem.
The
biotransformation
via
microorganisms
considered
an
ecofriendly
approach
for
its
detoxification.
present
study
focused
on
isolation
indigenous
bacterium
from
municipal
solid
waste
dumpsite
soils
with
relatively
higher
resistance
(>1000
mg
L−1
broth)
and
97%
reduction
(100
L−1)
under
conditions
37
°C,
pH
7,
120
rpm
48
h.
biochemical
16
S
rRNA
gene
sequencing
showed
that
isolate
exhibits
99%
similarity
Mammaliicoccus
sciuri.
Partial
reductase
sequences
were
retrieved,
showing
96%
to
NADPH-dependent
FMN
reductases.
Fourier
transform
infrared
spectroscopy,
scanning
electron
microscopy-energy
dispersive
X-ray
spectroscopy
presence
Cr
products
bacterial
cells.
photoelectron
spectroscopic
analysis
confirmed
valence
state
residual
Cr(III).
Furthermore,
quantitative
localization
reduced
transmission
microscopic
intracellular
accumulation
A
multifaceted
mechanism,
including
surface
adsorption
bioaccumulation
after
biotransformation,
confers
potential
identity
strain
sciuri
jv5
toward
remediation
polluted
environment
niche.
Microorganisms,
Год журнала:
2024,
Номер
12(8), С. 1621 - 1621
Опубликована: Авг. 8, 2024
Antimicrobial
resistance
(AMR)
is
a
growing
global
health
challenge,
compromising
bacterial
infection
treatments
and
necessitating
robust
surveillance
mitigation
strategies.
The
overuse
of
antimicrobials
in
humans
farm
animals
has
made
them
hotspots
for
AMR.
However,
the
spread
AMR
genes
wildlife
environment
represents
an
additional
turning
these
areas
into
new
hotspots.
Among
bacteria
considered
to
be
high
concern
public
health,
Arsenic
(As),
found
in
diverse
ecosystems,
poses
major
public
health
risks
various
parts
of
the
world.
Arsenotrophic
bacteria
contaminated
environments
help
reduce
toxicity
by
converting
arsenite
(AsIII)
to
less
harmful
arsenate
(AsV).
We
assumed
that
Achromobacter
aegrifaciens
strains
from
As-contaminated
tubewell
water
and
soil
would
share
similar
genomic
characteristics
associated
with
arsenic
detoxification
bioremediation.
To
investigate
this,
we
employed
both
culture-dependent
culture-independent
viz.
whole
genome
sequencing
(WGS)
methods
thoroughly
elucidate
phenotypic
genotypic
features
two
A.
isolated
(BAW48)
(BAS32)
samples
collected
Bogura
district
Bangladesh.
Both
BAW48
BAS32
isolates
demonstrated
As(III)
oxidation
KMNO4
test,
which
was
corroborated
molecular
analysis
confirming
presence
aioA
arsB
genes
strains.
These
were
be
phylogenetically
related
many
spp.,
biological
inorganic
reactors,
environmental
soils,
sediments
human
clinical
across
geographical
regions.
Moreover,
possessed
distinct
heavy
metal
resistance
conferring
Co,
Zn,
Cu,
Cd,
Hg,
As,
Cr.
Three
As
gene
clusters
such
as
oxidizing
aioBA,
reducing
arsRCDAB
MMA(III)
ars
(arsHCsO)
cluster
predicted
genomes
aegrifaciens.
Further
analyses
revealed
profiles
strains,
mobile
genetic
elements,
antimicrobials
genes,
virulence
metabolic
features.
Pangenome
synteny
showed
are
evolutionary
other
but
closely
one
another.
The
data
confirmed
can
oxidize
detoxify
metals
like
suggesting
their
potential
for
findings
align
our
assumption
provide
a
basis
developing
sustainable
solutions
bioremediation
efforts
environments.
