Amyloid Targeting-Gold Nanoparticles-Assisted X-ray Therapy Rescues Islet β-Cells from Amyloid Fibrils and Restores Insulin Homeostasis

ACS Nano, Год журнала: 2025, Номер 19(5), С. 5460 - 5474

Опубликована: Янв. 30, 2025

Type-2-diabetes is a metabolic disorder where misfolding and oligomerization of islet amyloid polypeptide (IAPP) around islet-β cells oligomerizes participates in the pathology. The oligomeric stage toxic but transitory leads to formation mature fibrils. pathological specifics fibrils are poorly understood. Here, we demonstrate that IAPP amyloids make gel-like transition, increasing viscosity local microenvironment encasing impeding their ability sense glucose release insulin. Using dual-targeted gold nanoparticles (AuNPs) capped with amyloid-fragments βCasein anti-IAPP antibodies, show X-ray irradiation AuNPs when bound results therapeutic remodelling amyloids, reduction solution, restoration glucose/insulin homeostasis. This study establishes can participate progressive pathology type-2-diabetes by suppressing insulin responsiveness at single islet-cell level. It also identifies model reversal using AuNPs-mediated therapy, this approach be rationally expanded other pathologies, such as Alzheimer's Parkinson's diseases.

Язык: Английский

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Assessing Drug Administration Techniques in Zebrafish Models of Neurological Disease

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(19), С. 14898 - 14898

Опубликована: Окт. 4, 2023

Neurological diseases, including neurodegenerative and neurodevelopmental disorders, affect nearly one in six of the world’s population. The burden resulting deaths disability is set to rise during next few decades as a consequence an aging To address this, zebrafish have become increasingly prominent model for studying human neurological diseases exploring potential therapies. Zebrafish offer numerous benefits, such genetic homology brain similarities, complementing traditional mammalian models serving valuable tool screening drug discovery. In this comprehensive review, we highlight various delivery techniques systems employed therapeutic interventions zebrafish, evaluate their suitability. We also discuss challenges encountered process present advancements innovative techniques.

Язык: Английский

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Sub-chronic exposure to paroxetine disrupts ecologically relevant behaviours in fish

The Science of The Total Environment, Год журнала: 2024, Номер 917, С. 170405 - 170405

Опубликована: Янв. 26, 2024

The functional conservation of important selective serotonin reuptake inhibitor (SSRI) targets in non-target organisms raises concerns about their potential adverse effects on the ecosystems. Although environmental levels SSRIs like paroxetine (PAR) have risen, knowledge regarding long-term exposure to PAR is limited. This study investigated impact sub-chronic (21 days) two sub-lethal concentrations (40 and 400 μg/L) behaviour adult zebrafish different scenarios: basal activity (under dark light conditions), stress response (evoked by sudden transitions) recovery. A new framework was employed for integrative fish's swimming performance based innate ability respond shifts. Several swimming-associated parameters (e.g., total distance, time inactivity, angles) thigmotaxis were monitored an integrated analysis each scenario. Data revealed reduced activity, impaired behavioural alterations recovery PAR-exposed fish. An anxiolytic effect particularly noticeable fish at μg/L (from light) dark) exposed 40 μg/L. detected PAR-induced modifications suggest a disruption brain glucocorticoid signalling that may implications individual level changing responses predators), with repercussions population community levels. Therefore, applied protocol proved sensitive detecting changes induced PAR.

Язык: Английский

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Design of an automated robotic microinjection system for batch injection of zebrafish embryos and larvae

Microsystems & Nanoengineering, Год журнала: 2024, Номер 10(1)

Опубликована: Янв. 29, 2024

This paper presents the design of a vision-based automated robotic microinjection system for batch injection both zebrafish embryos and larvae. A novel visual recognition algorithm based on an automatic threshold excessive dilatation is introduced to accurately identify center larval yolks. corresponding software developed using producer-consumer model as framework structure, friendly user interface designed allow operators choose from range desired functions according their different needs. In addition, microstructural agarose device fabricated simultaneously immobilize mixed batches Moreover, prototype by integrating hardware devices with algorithms. An experimental study conducted verify performance system. The results show that reported can larvae efficiently complete tasks mixtures success rate 92.05% in 13.88 s per sample. Compared manual existing systems, proposed demonstrates merits versatility, excellent efficiency, high rate, survival sufficient stability.

Язык: Английский

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Ecotoxicological Effects of TiO2 P25 Nanoparticles Aqueous Suspensions on Zebrafish (Danio rerio) Eleutheroembryos

Nanomaterials, Год журнала: 2024, Номер 14(4), С. 373 - 373

Опубликована: Фев. 17, 2024

Among nanoparticles (NPs), titanium dioxide is one of the most highly manufactured worldwide and widely used in multiple products for both industrial use personal care products. This increases probability release into aquatic environments, potentially affecting these ecosystems. The present study aimed to evaluate TiO2 P25 NP toxicity zebrafish embryos eleutheroembryos by evaluating LC50, hatching rate, embryo development, chemical analysis concentration accumulated eleutheroembryo tissues. Zebrafish ~2 h post-fertilization (hpf) were exposed 75, 100, 150, 200, 250 mg/L NPs 48 96 h. A total 40–60 placed each Petri dish respective treatments. Three replicates treatment group. Ti4+ concentrations determined inductively coupled plasma optical emission spectrometry (ICP-OES), a conversion factor was calculate highest calculated larvae 1.0199 after 1.2679 exposure. toxicological results indicated that did not have significant effect on mortality but an their development. LC20 LC30 experimentally, LC50 LC80 estimated using four different methods. Up 11% also presented physical malformations. These effects can be detrimental species affect Physical malformations observed all treatments, indicating teratogenic effects.

Язык: Английский

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Deep Learning Dives: Predicting Anxiety in Zebrafish Through Novel Tank Assay Analysis

Physiology & Behavior, Год журнала: 2024, Номер unknown, С. 114696 - 114696

Опубликована: Сен. 1, 2024

Язык: Английский

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Identifying knowledge gaps in understanding the effects of selective serotonin reuptake inhibitors (SSRIs) on fish behaviour

Chemosphere, Год журнала: 2023, Номер 335, С. 139124 - 139124

Опубликована: Июнь 5, 2023

Selective serotonin reuptake inhibitors (SSRIs) are a class of antidepressants increasingly prescribed to treat patients with clinical depression. As result the significant negative impact COVID-19 pandemic on population's mental health, its consumption is expected increase even more. The high these substances leads their environmental dissemination, evidence ability compromise molecular, biochemical, physiological, and behavioural endpoints in non-target organisms. This study aimed provide critical review current knowledge regarding effects SSRI fish ecologically relevant behaviours personality-dependent traits. A literature shows limited data concerning personality responses contaminants how such could be influenced by SSRIs. lack information may attributable widely adopted standardized protocols for evaluating fish. existing studies examining SSRIs across various biological levels overlook intra-specific variations behaviour physiology associated different patterns or coping styles. Consequently, some remain undetected, as styles capacity handle stressors. oversight potentially long-term ecological implications. Data support need more understand traits they impair fitness-related behaviours. Given considerable cross-species similarity dimensions, collected allow new insights into correlation between animal fitness.

Язык: Английский

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A northern snakehead (Channa argus) model of intestinal inflammation induced by Aeromonas hydrophila: Construction and transcriptome analysis

Aquaculture, Год журнала: 2023, Номер 580, С. 740323 - 740323

Опубликована: Ноя. 10, 2023

Язык: Английский

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MEndoB, a chimeric lysin featuring a novel domain architecture and superior activity for the treatment of staphylococcal infections

mBio, Год журнала: 2024, Номер 15(2)

Опубликована: Янв. 26, 2024

One of the most pressing challenges our era is rising occurrence bacteria that are resistant to antibiotics. Staphylococci prominent pathogens in humans, which have developed multiple strategies evade effects Infections caused by these resulted a high burden on health care system and significant loss lives. In this study, we successfully engineered lytic enzymes exhibit an extraordinary ability eradicate staphylococci. Our findings substantiate importance meticulous lead candidate selection identify therapeutically promising peptidoglycan hydrolases with unprecedented activity. Hence, they offer new avenue for treating staphylococcal infections.

Язык: Английский

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Reversible Influence of Hemipiperazine Photochromism on the Early Development of Zebrafish Embryo

ChemBioChem, Год журнала: 2024, Номер 25(8)

Опубликована: Март 5, 2024

This study explores the potential of controlling organismal development with light by using reversible photomodulation activity in bioactive compounds. Specifically, our research focuses on plinabulin 1, an inhibitor tubulin dynamics that contains a photochromic motif called hemipiperazine. The two isomeric forms, Z-1 and E-1, can partially interconvert light, yet show remarkable thermal stability darkness. Z-isomer exhibits higher cytotoxicity due to stronger binding α-tubulin's colchicine site. less toxic E-1 form, considered "pro-drug", be isolated vitro stored. Upon activation blue or cyan it predominantly generates more form. Here we demonstrate 1 effectively photomodulate epiboly, critical microtubule-dependent cell movement during gastrulation zebrafish embryos. highlights for precise control cellular activities development.

Язык: Английский

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