Bacterial biota associated with the invasive insect pest Tuta absoluta (Meyrick)

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Апрель 9, 2024

Abstract Tuta absoluta (the tomato pinworm) is an invasive insect pest with a highly damaging effect on tomatoes causing between 80 and 100% yield losses if left uncontrolled. Resistance to chemical pesticides have been reported in some T. populations. Insect microbiome plays important role the behavior, physiology, survivability of their host. In bid explore develop alternative control method, associated this was studied. study, we unraveled bacterial biota larvae adults by sequencing analyzing 16S rRNA V3-V4 gene regions using Illumina NovaSeq PE250. Out 2,092,015 amplicon sequence variants (ASVs) recovered from 30 samples (15 15 adults), 1,268,810 823,205 ASVs were obtained adults, respectively. A total 433 genera shared larval while 264 139 unique Amplicon metagenomic analyses sequences showed dominance phylum Proteobacteria adult Firmicutes dominated samples. Linear discriminant analysis size (LEfSe) comparison revealed Pseudomonas, Delftia Ralstonia be differentially enriched Enterococcus , Enterobacter Lactococcus Klebsiella Wiessella abundant larvae. The diversity indices that communities not different collected geographical regions. However, significantly differed based sample type adults. co-occurrence network correlated taxa strong interaction microbial communities. functional FAPROTAX denitrification, arsenite oxidation, methylotrophy methanotrophy as active groups microbiomes. Our results core taxonomic, functional, interacting microbiota these indicate harbor similar but transitory set bacteria. provide novel insight basis for exploring microbiome-based biocontrol strategy well ecological significance identified discussed.

Язык: Английский

---

The Impact of High-Temperature Stress on the Growth and Development of Tuta absoluta (Meyrick)

Insects, Год журнала: 2024, Номер 15(6), С. 423 - 423

Опубликована: Июнь 5, 2024

Insect life processes and reproductive behaviors are significantly affected by extremely high temperatures. This study focused on Tuta absoluta, which poses a severe threat to tomato cultivars. The effects of intense heat stress the growth, development, oviposition, longevity T. absoluta were investigated. investigation encompassed various developmental stages, including eggs, pupae, adults. revealed that egg hatching pupa emergence rates reduced at temperature 44 °C maintained for 6 h. adults emerged after pupal stages exposed h was compared control. Notably, there no significant variation in adult fecundity egg-stage exposure However, all treatments exhibited control temperatures during stage. Adult survival 40 3 74.29% 22.40%, respectively, dramatically less than control, 100%. differences noted terms production. These results offer better understanding complex interactions between extreme history traits thereby offering valuable insights implementing management strategies alleviate its impact crops response climate change.

Язык: Английский

---

Phytochemical properties and insecticidal activity of hexane extract of Melia azedarach fruit against Tuta absoluta (Meyrick)

Archives of Phytopathology and Plant Protection, Год журнала: 2025, Номер unknown, С. 1 - 14

Опубликована: Янв. 11, 2025

The tomato leaf miner (TLM), Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), is important pest on tomato, potato and other Solanaceous with a huge economic importance. Management of the can be problematic, especially when infestation pressure high. Phytochemical contact toxicity hexane extract Melia azedarach L. (MA) fresh fruit developmental stage egg, 2nd larval instars pupae T. were evaluated in laboratory condition, 27 ± 2 °C, 65 5% relative humidity 16 Light:8 Dark hours photoperiods. findings showed that M. azadrach harmful to at various stages, including larvae pupae. major components 9,12-Octadecadienoic acid (Z,Z)- methyl ester (69.375%), 9-Octadecenoic (Z)- (17.231%) Hexadecanoic acid, (7.361%), respectively. Analysis probit data LC50 for MA instar larvae, found 948.93, 346.72 1.75 μl/ml, Sublethal concentrations had significant impact nutrition (p < 0.001). results controls open closed environments recommended protecting environment health users.

Язык: Английский

---

Monitoring of Tomato Leaf Miner (Tuta absoluta) and Assessment of Management Practices Adopted in Tanahun, Nepal

Trends in Agricultural Sciences, Год журнала: 2025, Номер 4(1), С. 1 - 12

Опубликована: Янв. 27, 2025

Язык: Английский

---

An overview of sustainable management strategies for Tuta absoluta

International Journal of Pest Management, Год журнала: 2025, Номер unknown, С. 1 - 24

Опубликована: Фев. 4, 2025

Язык: Английский

---

Genome-Wide Identification of the Genes of the Odorant-Binding Protein Family Reveal Their Role in the Olfactory Response of the Tomato Leaf Miner (Tuta absoluta) to a Repellent Plant

Agronomy, Год журнала: 2024, Номер 14(1), С. 231 - 231

Опубликована: Янв. 22, 2024

The remarkable biological and evolutionary adaptations of insects to plants are largely attributed the powerful chemosensory systems insects. tomato leaf miner (Tuta absoluta) is a destructive invasive pest with global distribution that poses serious threat production nightshade crops, especially tomatoes. Functional can attract or repel insect pests by releasing volatiles interact olfactory system insects, thereby reducing damage target crops. However, there limited research on interaction between T. absoluta genes functional plants. In this study, 97 members putative odorant-binding protein (OBP) family have been identified in whole genome absoluta. Phylogenetic analysis involving various Lepidopteran Dipteran species, including D. melanogaster, revealed OBP gene families present conserved clustering patterns. Furthermore, Plus-C subfamily showed extremely significant expansion. Moreover, expression levels varied significantly different developmental stages; is, highest number were expressed adult stage, followed larval fewer high abundance egg stage. On other hand, through Y-tube olfactometer, we plant—Plectranthus tomentosa—that repels Finally, screened response P. tomentosa at genomic level using RT-qPCR. These results laid good foundation for controlling further studying genes.

Язык: Английский

---

Farmers' perception and adoption of management practices against tomato damage by tomato leaf miner (Tuta absoluta) in Pokhara, Nepal

Archives of Agriculture and Environmental Science, Год журнала: 2024, Номер 9(2), С. 317 - 323

Опубликована: Июнь 25, 2024

The tomato leaf miner, Tuta absoluta, poses a significant threat to cultivation globally, with notable impact on yields. This study investigates the perceptions and practices of farmers in Pokhara, Nepal, regarding T. absoluta management. We aimed assess current adoption management strategies, identify influencing factors, uncover knowledge gaps among farmers. surveyed 69 randomly selected households involved using semi-structured questionnaire. survey collected data observations damage, (chemical, physical, pheromone-based), awareness integrated pest (IPM) ecosystem services. Our findings reveal that 94% observed damage their fields, predominant reliance chemical pesticides (86.96%). Awareness environmentally friendly practices, such as IPM, were notably lacking. Factors age household head, crop rotation, cropping systems, family type significantly influenced various practices. While 44.93% used pheromones, 31% recognized them effective. highlights severe Pokhara insufficiency Bridging eco-friendly approaches, pheromones biological controls, is crucial. emphasize need tailor extension services by considering sociodemographic factors promoting Ultimately, our will contribute more sustainable agricultural settings.

Язык: Английский

---

Chitin Deacetylase 1 Gene as an Optimal RNAi-Based Target for Controlling the Tomato Leaf Miner Tuta absoluta

Insects, Год журнала: 2024, Номер 15(11), С. 838 - 838

Опубликована: Окт. 25, 2024

Chitin is a critical component of both the exoskeleton and internal structures insects, which can protect insects from mechanical damage, dehydration pathogen infection, plays significant role in molting process. deacetylases (CDAs), key enzymes involved chitin metabolism, are widely distributed among arthropods microorganisms. In this study, we identified

Язык: Английский

---

To Effectively Control Tuta absoluta (Meyrick) and Improve Crop Yields, Consider Exploring the Benefits of Intercropping Tomatoes with Garlic

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 11, 2024

Diversifying agroecosystems is crucial for maintaining ecosystem functions, including pest control, beneficial insect diversity, and soil fertility. In this study, the impact of intercropping tomato (T) (Lycopersicon esculentum (Mill.) garlic (G) (Allium sativum L.) in three designs 4E:4G, 4E:8G, 4E:12G (row ratios) were assessed on density leafminer (TLM), Tuta absoluta (Meyrick), fruit infestations, diversity tomato-dwelling predators, parasitism rate, crop yields compared with sole crops over two growing seasons 2022 2023. Three intercrops caused a significant reduction number TLM eggs, larvae, galleries, as well percentage infested fruits to plots. The abundance primary Nesidiocoris tenuis (Reuter) Orius niger (Wolff), higher Furthermore, annual predators enhanced than Besides, there was noticeable enhancement Shannon index (the values 1.98 4T: 4G 2.31 12G 2023) 1.68 1.82 2023 land equivalent ratio (LER) showed notable improvement, ranging from 1.19 1.32 intercrop, crops. Therefore, farmers may consider implementation aforementioned strategy integrated management T. infestations their fields.

Язык: Английский

---

Understanding Greenhouse Tomato (Solanum lycopersicum L.) Growers’ Perceptions for Optimal Phthorimaea absoluta (Meyrick) Management—A Survey in Greece

Agriculture, Год журнала: 2024, Номер 14(12), С. 2291 - 2291

Опубликована: Дек. 13, 2024

The tomato (Solanum lycopersicum L.) leafminer, Phthorimaea absoluta (Meyrick) (Lepidoptera: Gelechiidae), is an extremely invasive pest and threatening production worldwide. Several strategies have been developed implemented for managing P. absoluta. Effective control based on integrated management (IPM) schemes include different cultural practices techniques. This study seeks to (a) examine the perspectives, attitudes, opinions of greenhouse producers in Greece regarding methods (b) determine whether perceived damage caused by could be predicted implementation various strategies. A questionnaire was employed ascertain views beliefs participants. comprised five-point Likert scale questions. Principal component analysis (PCA) binary logistic regression were applied relationship between reported insect utilization findings indicate that greater adoption alternative higher levels adherence preventive measures against are associated with a perception reduced damage. Conversely, individuals strong reliance pesticides use more likely perceive level Ultimately, as growers trying deal absoluta, education research other alternatives help reduction chemical use, promoting environmentally friendly practices. highlight over sustainable production. can guide future extension efforts develop tailored approaches Greek similar environments.

Язык: Английский

---