Mechanism of Bazi Bushen capsule in delaying the senescence of mesenchymal stem cells based on network pharmacology and experimental validation

Heliyon, Год журнала: 2024, Номер 10(6), С. e27646 - e27646

Опубликована: Март 1, 2024

Ageing is becoming an increasingly serious problem; therefore, there urgent need to find safe and effective anti-ageing drugs.AimsTo investigate the effects of Bazi Bushen capsule (BZBS) on senescence mesenchymal stem cells (MSCs) explore its mechanism action.MethodsNetwork pharmacology was used predict targets BZBS in delaying MSCs. For vitro studies, MSCs were treated with D-gal, BZBS, NMN, cell viability, senescence, stemness-related genes, cycle studied using counting kit-8 (CCK-8) assay, SA-β-galactosidase (SA-β-gal) staining, Quantitative Real-Time PCR (qPCR) flow cytometry (FCM), respectively. Alkaline phosphatase (ALP), alizarin red, oil red staining determine osteogenic lipid differentiation abilities Finally, expression senescence-related genes cyclin-related factors detected by qPCR western blotting.ResultsNetwork pharmacological analysis suggested that delayed interfering cycle. Our studies could significantly increase viability (P < 0.01), decrease quantity β-galactosidase+ downregulate p16 p21 0.05, P improve adipogenic differentiation, upregulate Nanog, OCT4 SOX2 0.01) senescent Moreover, reduced proportion G0/G1 phase enhanced CDK4, Cyclin D1, E2F1 0.01, respectively). Upon treatment HY-50767A, a CDK4 inhibitor, upregulation no longer observed group.ConclusionsBZBS can protect against D-gal-induced which may be associated regulation via D1/CDK4/E2F1 signalling pathway.

Язык: Английский

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Targeting Lkb1-Ampk-Sirt1-Induced Autophagy and Mitophagy Pathways Improve Cerebrovascular Homeostasis in App/Ps1 Mice

Опубликована: Янв. 1, 2025

Язык: Английский

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Targeting LKB1–AMPK–SIRT1–induced autophagy and mitophagy pathways improves cerebrovascular homeostasis in APP/PS1 mice

Free Radical Biology and Medicine, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

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Bazi Bushen capsule attenuates cardiac systolic injury via SIRT3/SOD2 pathway in high-fat diet-fed ovariectomized mice

Heliyon, Год журнала: 2024, Номер 10(11), С. e32159 - e32159

Опубликована: Май 31, 2024

BackgroundBazi Bushen capsule (BZBS) is a traditional Chinese herbal medicine that clinically used to treat fatigue and forgetfulness. However, it still unclear whether how BZBS affects menopausal women's heart function decline. This study aimed examine the effect of on cardiac in high-fat diet-fed ovariectomy (HFD-fed OVX) mouse model elucidate underlying mechanism this effect.MethodsThe experimental animals were divided into five groups: sham group, HFD-fed OVX (0.7, 1.4, 2.8g/kg) intervention groups. Senescence β-galactosidase staining echocardiography evaluate function. SwissTargetPrediction, KEGG GO enrichment analysis screen BZBS. The morphological functional changes mitochondria molecular assessed by transmission electron microscopy, western blotting biochemical assays. STRING database was protein-protein interaction (PPI) network. Molecular docking studies employed predict interactions specific compounds with their protein targets.ResultsBZBS treatment ameliorated senescence systole injury mice. revealed 530 targets 14 main components enriched mainly oxidative stress-associated pathway, which confirmed finding prevents abnormal stress damage Furthermore, showed are broadly related Sirtuins family. And upregulated SIRT3 elevated activity SOD2 hearts mice, also verified vitro. Additionally, we imperatorin osthole from expression directing transcription factors HDAC1, HDAC2, BRD4, regulate SIRT3.ConclusionThis research shows antioxidative cardioprotective role mice involves an increase SIRT3/SOD2 may play central roles process.

Язык: Английский

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Gut Aging: A wane from the normal to repercussion and Gerotherapeutic strategies

Heliyon, Год журнала: 2024, Номер 10(19), С. e37883 - e37883

Опубликована: Сен. 13, 2024

Globally, age-related diseases represent a significant public health concern among the elderly population. In aging, healthy organs and tissues undergo structural functional changes that put aged adults at risk of diseases. Some include cancer, atherosclerosis, brain disorders, muscle atrophy (sarcopenia), gastrointestinal (GIT) etc. organs, decline in stem cell function is starting point many conditions extremely important GIT disorder development. Many studies have established aging affects cells their surrounding supportive niche components. Although there advancement treating intestinal rising population coupled with higher occurrence chronic gut ailments necessitates more effective therapeutic approaches to preserve health. Notable strategies such as Western medicine, traditional Chinese other health-promotion interventions been reported several hold promise mitigating disorders. This review highlights findings across various facets focus on aging-associated components, thus deviation from normal repercussion, well essential mitigate aging.

Язык: Английский

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Liposomal α-cyperone targeting bone resorption surfaces suppresses osteoclast differentiation and osteoporosis progression via the PI3K/Akt axis

Nano Research, Год журнала: 2023, Номер 17(4), С. 2949 - 2959

Опубликована: Окт. 31, 2023

Язык: Английский

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Gut Aging: A Wane from the Normal to Repercussion and Gerotherapeutic Strategies

Опубликована: Янв. 1, 2024

Язык: Английский

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