Journal of Ethnopharmacology, Год журнала: 2024, Номер 338, С. 119098 - 119098
Опубликована: Ноя. 16, 2024
Язык: Английский
Heliyon, Год журнала: 2024, Номер 10(6), С. e27506 - e27506
Опубликована: Март 1, 2024
Osteoarthritis (OA) is a prevalent degenerative joint disease characterized by cartilage degradation, inflammatory arthritis, and dysfunction. Currently, there lack of effective early diagnostic methods treatment strategies for OA. Bioinformatics biomarker research provide new possibilities detection personalized therapy In this study, we investigated the molecular mechanisms OA important signaling pathways involved in progression through bioinformatics analysis. Firstly, using limma package, analyzed differentially expressed genes (DEGs) between normal healthy samples tissue samples. These DEGs were found to be primarily biological processes such as extracellular matrix (ECM) binding, immune receptor activity, cytokine well including receptors, ECM-receptor interaction, PI3K-Akt. Gene set enrichment analysis revealed that group, AMPK, B cell receptor, IL-17, PPAR downregulated, while calcium pathway, adhesion molecules, TGF-β Wnt pathway upregulated. Additionally, constructed co-expression module network WGCNA identified key modules associated with OA, from which selected 7 most predictive characteristic genes. Among them, ANTXR1, KCNS3, SGCD, LIN7A correlated level infiltration. This study elucidates underlying development identifies potential markers therapeutic targets, providing insights diagnosis
Язык: Английский
Процитировано
6
Journal of Inflammation Research, Год журнала: 2025, Номер Volume 18, С. 827 - 846
Опубликована: Янв. 1, 2025
The phenomenon of "kidney-brain crosstalk" has stimulated scholarly inquiry into the correlations between kidney injury (KI) and Alzheimer's disease (AD). Nonetheless, precise interactions shared mechanisms KI AD have yet to be fully investigated. primary goal this study was investigate link AD, with a specific focus on identifying diagnostic biomarkers for KI-related AD. first step present use Mendelian randomization (MR) analysis followed by verification in vivo vitro experiments. Subsequently, bioinformatics machine learning techniques were used identify KI-associated ferroptosis-related genes (FRGs) which validated following Moreover, relationship hub immune infiltration assessed using CIBERSORT, potential drugs or small molecules associated core identified via DGIdb database. MR showed that may risk factor Experiments combination D-galactose aluminum chloride found induce both ferroptosis emerging as bridge facilitate crosstalk Besides, we EGFR RELA significant value. These are NK_cells_resting B_cells_memory could targeted intervention treating gefitinib plumbagin. Our elucidates an important pathway kidney-brain crosstalk. Notably, plumbagin therapeutic candidates intervening targeting RELA.
Язык: Английский
Процитировано
0
Journal of Applied Polymer Science, Год журнала: 2025, Номер unknown
Опубликована: Март 4, 2025
ABSTRACT Recently, there has been a surge in scholarly interest regarding the application of sophisticated materials technology to expedite wound healing, particularly through integration nanocomposites endowed with multifaceted functionalities augment efficacy care products. In order propose an external power‐free healing dressing electrical stimulation function, polyvinylidene fluoride (PVDF) nanofibers incorporating graphene oxide (GO) at varying concentrations were fabricated via electrospinning technique. Scanning electron microscopy (SEM) was employed reveal morphology composite nanofibers. Fourier transform infrared (FTIR) spectroscopy and X‐ray diffraction (XRD) analyses confirmed transition PVDF from α phase β phase. The antibacterial PVDF/GO against Staphylococcus aureus rigorously examined. Results indicated marked enhancement correlation increasing content GO. Moreover, piezoelectric property assessments, cytotoxicity, hemolysis tests meticulously performed. outcomes suggested that containing 0.5 w/w% GO (PVDF/GO‐0.5) demonstrated superior performance across all evaluated metrics, terms mechanical properties, characteristics, efficacy. These findings imply PVDF/GO‐0.5 possess capability mimic endogenous electric field, which is beneficial boost cellular migration proliferation, thereby accelerating process. Overall, innovative proposed this study can be considered highly promising candidate field tissue engineering.
Язык: Английский
Процитировано
0
International Journal of General Medicine, Год журнала: 2024, Номер Volume 17, С. 2433 - 2443
Опубликована: Май 1, 2024
Introduction: Chondrocyte degeneration and senescence are characteristics of osteoarthritis (OA) other joint degenerative diseases, ferroptosis has been observed to regulate the development OA. However, role N 6 -methyladenosine (m A) modification in OA remains unclear. Methods: This study performed series assays investigate function m A reader IGF2BP1 ferroptosis, including quantitative analysis, Iron (Fe 2+ ) release Malondialdehyde (MDA) measurement, lipid peroxidation (ROS) detection Glutathione (GSH) measurement. The molecular interaction mechanism analysis was by Luciferase reporter assay, mRNA stability RNA immunoprecipitation (RIP) assay. Results: These results indicate that is upregulated IL-1β-induced chondrocytes. Functionally, silencing represses iron accumulation, malondialdehyde, reactive oxygen species (ROS). Mechanistically, among potential downstream targets, matrix metalloproteinase-3 (MMP3) harbor a significant modified site 3'-UTR. combines with MMP3 through binding sites, thereby enhancing stability. Discussion: In conclusion, our findings revealed functions mechanisms regulator chondrocyte's providing novel target for treatment. Keywords: osteoarthritic cartilage, IGF2BP1,
Язык: Английский
Процитировано
2
Heliyon, Год журнала: 2024, Номер 10(17), С. e36650 - e36650
Опубликована: Авг. 23, 2024
The increasing prevalence of multi-morbidities, particularly the incidence breast cancer in diabetic/osteoarthritic patients emphasize on need for exploring underlying molecular mechanisms resulting carcinogenesis. To address this, present study employed a systems biology approach to identify switch genes pivotal crosstalk between diseased states multi-morbid conditions. Hub previously reported type 2 diabetes mellitus (T2DM), osteoarthritis (OA), and triple negative (TNBC), were extracted from published literature fed into an integrated bioinformatics analyses pipeline. Thirty-one hub common all three diseases identified. Functional enrichment showed these mainly enriched immune metabolism associated terms including advanced glycation end products (AGE) pathways, neoplasm, system signalling adipose tissue. T2DM-OA-TNBC interactome was subjected protein-protein interaction network meta hub/clustered genes. These prioritized wired disease map presenting axes gain insight disease-disease interactions. Deciphering bases intertwined metabolic may potentiate discovery biomarkers critical identifying targeting immuno-metabolic origin disease.
Язык: Английский
Процитировано
2
Frontiers in Medicine, Год журнала: 2024, Номер 11
Опубликована: Ноя. 5, 2024
Osteoarthritis (OA) is a progressive degenerative disorder impacting bones and joints, worsened by chronic inflammation, immune dysregulation, mechanical stress, metabolic disturbances, various other contributing factors. The complex interplay of cartilage damage, loss, impaired repair mechanisms remains critical formidable aspect OA pathogenesis. At the genetic level, multiple genes have been implicated in modulation chondrocyte metabolism, displaying both promotive inhibitory roles. Recent research has increasingly focused on influence non-coding RNAs regulation distinct cell types within bone tissue OA. In particular, an expanding body evidence highlights regulatory roles microRNAs chondrocytes. This review aims to consolidate most relevant associated with chondrocytes, as identified recent studies, elucidate their involvement processes ferroptosis. Furthermore, this study explores interactions between long (lncRNAs) circular (circRNAs) OA, emphasis microRNA-mediated mechanisms. Finally, gaps current are identified, offering strategic insights advance understanding pathophysiology guide therapeutic developments field.
Язык: Английский
Процитировано
2
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(15), С. 8212 - 8212
Опубликована: Июль 27, 2024
Ferroptosis is a form of iron-dependent regulated cell death caused by the accumulation lipid peroxides. In this review, we summarize research on impact ferroptosis disease models and isolated cells in various types arthritis. While most studies have focused rheumatoid arthritis (RA) osteoarthritis (OA), there limited spondylarthritis crystal arthropathies. The effects inducing or inhibiting strongly depend studied type. search for new therapeutic targets, chondrocytes might promising any type On other hand, induction may also lead to desired decrease synovial fibroblasts RA. Thus, must consider cell-type-specific Further investigation needed clarify these complexities.
Язык: Английский
Процитировано
1
Cell Biochemistry and Biophysics, Год журнала: 2024, Номер unknown
Опубликована: Сен. 22, 2024
Язык: Английский
Процитировано
0
Journal of Ethnopharmacology, Год журнала: 2024, Номер 338, С. 119098 - 119098
Опубликована: Ноя. 16, 2024
Язык: Английский
Процитировано
0