Intestinal
mucositis
(IM)
is
a
common
adverse
effect
of
chemotherapy,
limiting
its
clinical
application.
Edible
Chinese
medicine,
specifically
Codonopsis
pilosula-derived
CP-A,
with
anti-inflammatory
and
gastrointestinal
protective
effects.
The
present
work
was
to
investigate
CP-A's
role
in
ameliorating
IM
mechanism
using
vitro
rat
models.
Western
blot,
immunohistochemical
(IHC)
real-time
PCR
(RT-PCR)
analyses
were
used
assess
protein
expression
related
the
extracellular-regulated
kinases
(ERK)/myosin
light
chain
kinase
(MLCK)/myosin
2
(MLC2)
signaling
pathway
tight
junction
proteins.
Enzyme-linked
immunosorbent
assay
(ELISA)
conducted
measure
inflammatory
factors,
16S
rRNA
Amplicon
Sequencing
employed
for
cecum
content
analysis.
results
indicated
that
CP-A
could
restore
body
weight,
food
intake
histopathological
changes
rats.
Besides,
abnormal
MLCK
activation
induced
by
5-fluorouracil
(5-FU)
attenuated
via
ERK/MLCK/MLC2
pathway.
treatment
improved
levels
reduced
factor
expression.
On
other
hand,
intestinal
flora
experiment
demonstrated
intervention
increase
abundance
lactobacillus.
In
conclusion,
mitigates
5-FU-induced
inhibiting
pathway,
reducing
improving
mucosal
barrier
regulating
flora.
This
study
sheds
on
therapeutic
potential
treatment,
providing
insights
future
research.
Pharmaceuticals,
Год журнала:
2024,
Номер
17(3), С. 297 - 297
Опубликована: Фев. 26, 2024
Intestinal
mucositis
(IM)
is
a
common
adverse
effect
of
chemotherapy,
limiting
its
clinical
application.
Codonopsis
pilosula-derived
CP-A
(an
inulin-type
fructan)
an
edible
Chinese
medicine
with
anti-inflammatory
and
gastrointestinal
protective
effects,
which
may
be
useful
for
treating
IM.
Here,
we
explored
CP-A’s
role
in
ameliorating
IM
induced
by
5-fluorouracil
(5-FU)
investigated
the
underlying
mechanism
using
vitro
experiments
rat
models.
Western
blotting,
immunohistochemistry
(IHC),
real-time
PCR
(RT-PCR)
analyses
were
used
to
assess
protein
expression
related
extracellular-regulated
kinases
(ERK)/myosin
light
chain
kinase
(MLCK)/myosin
2
(MLC2)
signaling
pathway
tight
junction
proteins.
Inflammatory
factors
quantified
enzyme-linked
immunosorbent
assays
(ELISAs),
16S
rRNA
amplicon
sequencing
was
employed
cecum
content
analysis.
The
results
indicated
that
restored
body
weight
food
intake
reversed
histopathological
changes
rats.
Further,
abnormal
MLCK
activation
5-FU
attenuated
via
ERK/MLCK/MLC2
pathway.
treatment
improved
levels
reduced
inflammatory
factor
expression.
Moreover,
intervention
regulated
intestinal
microbiota
community
structure,
increasing
abundance
Lactobacillus
decreasing
Shigella.
In
conclusion,
mitigates
5-FU-induced
inhibiting
pathway,
reducing
factors,
improving
mucosal
barrier,
regulating
microbial
community.
This
study
highlights
therapeutic
potential
provides
insights
future
research.
Heliyon,
Год журнала:
2024,
Номер
10(3), С. e24617 - e24617
Опубликована: Фев. 1, 2024
Chrozophora
plicata
has
been
extensively
utilized
in
India
for
the
management
of
numerous
disorders.
The
effective
Phytoconstituents
derived
from
Ethyl
Acetate
Fraction
[EAFCP]
have
identified
as
Camptothecin
Agathisflavone,
Rutin,
Procynidine
B,
and
Apigenin.
These
detected
EAFCP
through
qualitative
analysis
using
LC-Q-TOF-MS/MS.
anti-inflammatory
properties
are
yet
to
be
determined.
Therefore,
aim
this
study
was
utilize
a
network
pharmacology-based
methodology
predict
potential
therapeutic
targets
setting
inflammation.
identification
inflammation
followed
by
acquisition
verified
EAFCP.
key
against
were
found
creating
target-functional
PPI
network,
GO
studies
conducted
on
core
order
assess
essential
signalling
pathways
involved
effects
A
total
38
significant
hub
associated
with
EAFCP's
identified.
proteins
retrieved
docking
investigation
based
findings,
which
aided
anticipating
interaction
between
components
targets.
vivo
revealed
that
had
notable
efficiency
decreasing
paw
edema
induced
carrageenan
rats.
evidence
we
gathered
collectively
offers
clarification
about
activity
EAFCP,
is
predominantly
linked
suppression
Cox
1,
2
pathway.
aforementioned
findings
highlight
could
Molecules,
Год журнала:
2024,
Номер
29(8), С. 1713 - 1713
Опубликована: Апрель 10, 2024
Traditional
Chinese
medicine
(TCM)
possesses
the
potential
of
providing
good
curative
effects
with
no
side
for
effective
management
slow
transit
constipation
(STC),
an
intestinal
disease
characterized
by
colonic
dyskinesia.
Mulberry
leaves
(Morus
alba
L.)
and
black
sesame
(Sesamum
indicum
L.),
referred
to
as
SH,
are
processed
conditioned
per
standardized
protocols.
SH
has
applications
food
medicine.
Accordingly,
we
investigated
therapeutic
in
alleviating
STC.
The
analysis
composition
identified
a
total
504
compounds.
intervention
significantly
improved
motility,
reduced
time
first
stool,
increased
antioxidant
activity,
enhanced
water
content,
thereby
effectively
colon
damage
caused
Transcriptome
revealed
treatment
STC
related
SOD1,
MUC2,
AQP1.
16S
rRNA
gene
sequences
indicated
notable
differences
abundance
10
bacteria
between
model.
Metabolomic
further
that
supplementation
levels
nine
metabolites
associated
Integrative
modulated
amino
acid
metabolism,
balanced
flora,
targeted
key
genes
(i.e.,
AQP1)
exert
its
effects.
also
inhibited
AQP1
expression
promoted
SOD1
MUC2
expression.
In
this
study,
we
investigated
how
different
proportions
blends
of
RG-I
pectic
polysaccharides
and
hesperidin
impact
the
gut
microbiota
metabolites
using
an
in
vitro
simulated
digestion
fermentation
model.
The
results
indicated
that
both
them
could
modulate
produce
beneficial
metabolites.
However,
their
particular
exhibited
remarkable
synergistic
effects
on
modulating
intestinal
microenvironment,
surpassing
observed
with
individual
components.
Specifically,
these
enhance
acidogenic
potential,
increase
short-chain
fatty
acids
production,
improve
bioavailability,
promote
microbial
diversity.
This
additive
effect
was
inseparable
from
role
microbiota.
Certain
bacteria,
such
as
Blautia,
Faecalibacterium,
Prevotella,
strong
preferences
for
those
blends,
thereby
contributing
to
host
health
through
participating
carbohydrate
flavonoid
metabolism.
