Intestinal
mucositis
(IM)
is
a
common
adverse
effect
of
chemotherapy,
limiting
its
clinical
application.
Edible
Chinese
medicine,
specifically
Codonopsis
pilosula-derived
CP-A,
with
anti-inflammatory
and
gastrointestinal
protective
effects.
The
present
work
was
to
investigate
CP-A's
role
in
ameliorating
IM
mechanism
using
vitro
rat
models.
Western
blot,
immunohistochemical
(IHC)
real-time
PCR
(RT-PCR)
analyses
were
used
assess
protein
expression
related
the
extracellular-regulated
kinases
(ERK)/myosin
light
chain
kinase
(MLCK)/myosin
2
(MLC2)
signaling
pathway
tight
junction
proteins.
Enzyme-linked
immunosorbent
assay
(ELISA)
conducted
measure
inflammatory
factors,
16S
rRNA
Amplicon
Sequencing
employed
for
cecum
content
analysis.
results
indicated
that
CP-A
could
restore
body
weight,
food
intake
histopathological
changes
rats.
Besides,
abnormal
MLCK
activation
induced
by
5-fluorouracil
(5-FU)
attenuated
via
ERK/MLCK/MLC2
pathway.
treatment
improved
levels
reduced
factor
expression.
On
other
hand,
intestinal
flora
experiment
demonstrated
intervention
increase
abundance
lactobacillus.
In
conclusion,
mitigates
5-FU-induced
inhibiting
pathway,
reducing
improving
mucosal
barrier
regulating
flora.
This
study
sheds
on
therapeutic
potential
treatment,
providing
insights
future
research.
The
addition
of
wine
lees
to
diets
can
make
up
for
the
deficiencies
caused
by
traditional
forages
in
beef
cattle
farming.
However,
effects
different
ratios
on
average
daily
weight,
gastrointestinal
microbial
community
structure
and
metabolites
Guanling
crossbred
have
been
rarely
studied.
This
study
assessed
feeds
containing
weight
gain,
structure,
elucidated
metabolic
responses
induced
lees.
Eighteen
cows
were
randomly
assigned
receive
fed
concentrate
(C
group),
feed
15%
(group
A),
or
30%
B)
60
days.
gain
group
A
B
increased
76.75%
57.65%,
respectively,
compared
with
C.
Microbial
analysis
showed
that
abundance
Prevotella_1
rumen,
decreased
Ruminococcaceae
UCG
011
Lachnospiraceae_FCS020_group
Tyzzerella_4,
Family_Xlll_AD3011_group,
Granulicella,
Eisenbergiella
cecum.
Metabolomics
analyses
concentrations
indole-3-ethanol
complexity
cecal
metabolism.
Notably,
linoleic
acid
metabolism
was
significantly
enriched
both
rumen
Mantel
test
indicated
adverse
WL
reduced
stimulating
acid,
α-linolenic
tryptophan,
these
changes
mediated
intestinal
microorganisms.
cecum
several
unfavorable
pathways
when
reached
30%,
which
likelihood
lesions.
shows
supplementation
alters
gut
microbiota
pathways,
improving
growth
health.
Moderate
levels
(15%)
enhance
health
beneficial
(e.g.,
alpha-linolenic
metabolism).
higher
inclusion
(30%)
may
activate
raising
risk
damage.
To
maximize
benefits
minimize
risks,
should
be
carefully
managed.
•
Feeding
lees(WL)
Changes
flora
feeding
WL.
Excess
lesions
cattle.
are
stimulated
reduce
regulating
tryptophan.
Nutrients,
Год журнала:
2024,
Номер
16(23), С. 4232 - 4232
Опубликована: Дек. 7, 2024
Gut
bacterial
dysbiosis
along
with
intestinal
mucosal
disruption
plays
a
critical
role
in
inflammatory
disorders
like
ulcerative
colitis.
Flavonoids
and
other
food
bioactives
have
been
studied
mice
models
as
alternative
treatments
minimal
side
effects.
However,
most
of
the
research
has
carried
out
mice-native
microbiota,
which
limits
comprehension
interaction
between
flavonoids
human-associated
bacteria.
Hence,
objective
our
study
was
to
determine
effect
healthy
microbiota
on
anti-colitic
activity
diets
rich
anthocyanins
(3-HF)
phlobaphenes
(3-DF).
Intestinal
mucositis
(IM)
is
a
common
adverse
effect
of
chemotherapy,
limiting
its
clinical
application.
Edible
Chinese
medicine,
specifically
Codonopsis
pilosula-derived
CP-A,
with
anti-inflammatory
and
gastrointestinal
protective
effects.
The
present
work
was
to
investigate
CP-A's
role
in
ameliorating
IM
mechanism
using
vitro
rat
models.
Western
blot,
immunohistochemical
(IHC)
real-time
PCR
(RT-PCR)
analyses
were
used
assess
protein
expression
related
the
extracellular-regulated
kinases
(ERK)/myosin
light
chain
kinase
(MLCK)/myosin
2
(MLC2)
signaling
pathway
tight
junction
proteins.
Enzyme-linked
immunosorbent
assay
(ELISA)
conducted
measure
inflammatory
factors,
16S
rRNA
Amplicon
Sequencing
employed
for
cecum
content
analysis.
results
indicated
that
CP-A
could
restore
body
weight,
food
intake
histopathological
changes
rats.
Besides,
abnormal
MLCK
activation
induced
by
5-fluorouracil
(5-FU)
attenuated
via
ERK/MLCK/MLC2
pathway.
treatment
improved
levels
reduced
factor
expression.
On
other
hand,
intestinal
flora
experiment
demonstrated
intervention
increase
abundance
lactobacillus.
In
conclusion,
mitigates
5-FU-induced
inhibiting
pathway,
reducing
improving
mucosal
barrier
regulating
flora.
This
study
sheds
on
therapeutic
potential
treatment,
providing
insights
future
research.
