Journal of Inflammation Research,
Год журнала:
2024,
Номер
Volume 17, С. 10785 - 10805
Опубликована: Дек. 1, 2024
Ulcerative
colitis
(UC),
a
major
type
of
inflammatory
bowel
disease,
is
characterized
by
chronic
inflammation
the
colonic
mucosa
and
submucosa.
Estrogen
receptor
β
(ERβ)
predominates
in
colon
exerts
anti-inflammatory
effects.
Ferroptosis,
recently
discovered
form
iron-dependent
programmed
cell
death,
implicated
pathogenesis
several
diseases,
including
UC.
However,
link
between
ferroptosis
actions
ERβ
UC
remains
to
be
elucidated.
Journal of Biochemical and Molecular Toxicology,
Год журнала:
2024,
Номер
38(10)
Опубликована: Сен. 17, 2024
Abstract
Ischemia‐reperfusion
(I‐R)
injury
is
the
most
common
cause
of
acute
kidney
injury.
In
experiments
involving
primary
human
renal
proximal
tubular
epithelial
cells
(RPTECs)
exposed
to
anoxia‐reoxygenation,
we
explored
hypothesis
that
mitochondrial
malate
dehydrogenase‐2
(MDH‐2)
inhibition
redirects
metabolism
from
mitochondria
cytoplasm,
towards
malate‐pyruvate
cycle
and
reversed
malate‐aspartate
shuttle.
Colorimetry,
fluorometry,
western
blotting
showed
MDH2
accelerates
enhancing
cytoplasmic
NADPH,
thereby
regenerating
potent
antioxidant
reduced
glutathione.
It
also
shuttle
potentially
diminished
reactive
oxygen
species
(ROS)
production
by
transferring
electrons,
in
form
NADH,
cytoplasm.
The
excessive
ROS
induced
anoxia‐reoxygenation
led
DNA
damage
protein
modification,
triggering
unfolded
response,
ultimately
resulting
apoptosis
senescence.
Additionally,
lipid
peroxidation,
which
may
contribute
process
ferroptosis.
Inhibiting
MDH‐2
proved
effective
mitigating
overproduction
during
rescuing
RPTECs
death
or
Thus,
targeting
holds
promise
as
a
pharmaceutical
strategy
against
I‐R
Journal of Inflammation Research,
Год журнала:
2024,
Номер
Volume 17, С. 10785 - 10805
Опубликована: Дек. 1, 2024
Ulcerative
colitis
(UC),
a
major
type
of
inflammatory
bowel
disease,
is
characterized
by
chronic
inflammation
the
colonic
mucosa
and
submucosa.
Estrogen
receptor
β
(ERβ)
predominates
in
colon
exerts
anti-inflammatory
effects.
Ferroptosis,
recently
discovered
form
iron-dependent
programmed
cell
death,
implicated
pathogenesis
several
diseases,
including
UC.
However,
link
between
ferroptosis
actions
ERβ
UC
remains
to
be
elucidated.