Astragaloside IV inhibits retinal pigment epithelial cell senescence and reduces IL-1β mRNA stability by targeting FTO-mediated m6A methylation

Phytomedicine, Год журнала: 2025, Номер 138, С. 156408 - 156408

Опубликована: Янв. 18, 2025

Язык: Английский

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Mettl3-Mediated N6-Methyladenosine Modification Mitigates Ganglion Cell Loss and Retinal Dysfunction in Retinal Ischemia–Reperfusion Injury by Inhibiting FoxO1-Mediated Autophagy

Investigative Ophthalmology & Visual Science, Год журнала: 2025, Номер 66(2), С. 58 - 58

Опубликована: Фев. 21, 2025

Purpose: N6-methyladenosine (m6A) modification has been implicated in ischemia–reperfusion injury various systems and several neurodegenerative diseases. Glaucoma is characterized by degeneration of retinal ganglion cells (RGCs) shares similar pathologic characteristics with (RIR) injury. However, the specific role m6A RIR unclear, involvement autophagy also remains controversial. Therefore, our study explored Methods: Male wild-type C57BL/6J mice (6–8 weeks old) were used to induce Retinal flat-mount immunofluorescence was performed assess RGC survival rate. Electroretinogram optomotor response conducted evaluate electrophysiologic function visual acuity. Autophagy level reflected Western blot transmission electron microscope images. M6A levels determined via dot blot. Methyltransferase-like protein 3 (Mettl3) forkhead box O1 (FoxO1) expressions tested Methylated RNA immunoprecipitation–quantitative PCR examine on FoxO1 mRNA. We employed 3-methyladenine rapamycin regulate Results: Inhibiting ameliorated loss preserved Additionally, a decrease Mettl3-mediated observed mice. By overexpressing Mettl3 intravitreal injection type 2 recombinant adeno-associated virus before injury, we established that overexpression can ameliorate dysfunction induced Furthermore, inhibited reduced expression upregulating modifications Conclusions: mitigates inhibiting FoxO1-mediated

Язык: Английский

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Exploring m6A methylation in skin Cancer: Insights into molecular mechanisms and treatment

Cellular Signalling, Год журнала: 2024, Номер unknown, С. 111420 - 111420

Опубликована: Сен. 1, 2024

Язык: Английский

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HO-1-Mediated Ferroptosis Regulates Retinal Neovascularization via the COX2/VEGFA Axis

Free Radical Biology and Medicine, Год журнала: 2024, Номер 226, С. 84 - 95

Опубликована: Ноя. 12, 2024

Язык: Английский

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