Anticancer Research,
Год журнала:
2024,
Номер
45(1), С. 73 - 79
Опубликована: Дек. 30, 2024
Background/Aim:
Organometallic
complexes
can
decrease
adhesion,
migration,
invasion
of
cancer
cells,
mainly
through
regulation
the
extracellular
matrix
and
therefore
act
against
metastases.
The
aim
was
to
investigate
anti-invasive
properties
a
rhenium-based
metal
compound,
rhenium(I)-diselenoether
(Re-diSe)
its
effects
on
metalloproteinase
MMP-2,
key
player
in
metastatic
processes,
cultured
MDA-MB231
triple-negative
breast
cells.
Materials
Methods:
Matrigel
utilized
assess
cell
adhesion
matrix.
Migration
capacity
evaluated
wound
healing
assay,
while
assays
were
conducted
using
transwell
assay
by
crystal
violet
dye.
MMP-2
levels
quantified
medium
fluorometric
kit
assay.
Cells
exposed
increasing
concentrations
drug
(5,
10,
20,
50
100
μM).
Three
time
points
exposure
cells
investigated
(48,
96
120
h).
Results:
Re-diSe
exhibited
effectiveness
even
at
doses
as
low
5
or
10
μM
all
biological
assays.
h
significantly
improved
effect
comparison
with
48
h.
Conclusion:
results
highlight
impact
Frontiers in Oncology,
Год журнала:
2024,
Номер
14
Опубликована: Ноя. 15, 2024
Proliferative
verrucous
leukoplakia
(PVL)
is
an
aggressive
and
distinct
type
of
oral
precancerous
lesion
characterized
by
warty
surfaced
white
plaque
diffusely
involving
mucosa.
Surgical
excision
the
treatment
choice.
However,
PVL
has
persistent
recurrent
growth
patterns,
requiring
multiple
surgical
procedures.
intervention
especially
challenging
if
extends
between
teeth
limiting
access.
These
interproximally
located
lesions
have
a
high
propensity
to
undergo
malignant
transformation.
We
report
case
53-year-old
man
with
covering
maxillary
mandibular
gingiva.
Despite
complete
excisions,
recurred,
focal
area
in
interproximal
gingiva
progressed
invasive
squamous
cell
carcinoma
marginal
resection.
The
remaining
areas
were
treated
topical
imiquimod
(toll-like
receptor
7
agonist)
for
six
months,
resulting
clinical
histological
resolution.
Topical
agents
can
cover
larger
surface
penetrate
areas.
Importantly,
it
allows
maximal
local
exposure
minimal
systemic
toxicity,
essential
long-term
prophylactic
use
agent
prevent
relapse.
Investigative Ophthalmology & Visual Science,
Год журнала:
2024,
Номер
65(14), С. 14 - 14
Опубликована: Дек. 5, 2024
Purpose:
Refractory
or
recurrent
retinoblastoma
results
from
acquired
chemoresistance
and
the
management
of
these
eyes
often
requires
surgical
removal.
Our
objective
was
to
develop
models
resistant
chemotherapy
by
exposing
cancer
cells
repeated
mimicking
clinical
scenario.
These
newly
were
used
evaluate
potential
novel
therapies.
Methods:
Chemoresistant
obtained
two
primary
cell
cultures
three
weekly
doses
melphalan
topotecan.
The
sensitivity
each
evaluated,
cross-resistance
topotecan,
melphalan,
carboplatin
assessed.
Genomic
alterations
differential
expression
efflux/influx
transporters
between
chemoresistant
parental
analyzed.
Subsequently,
both
repurposed
agents
digoxin,
methylene
blue,
gemcitabine
Results:
Four
successfully
established,
showing
significantly
higher
half-maximal
inhibitory
concentration
(IC50)
values
for
topotecan
compared
their
corresponding
(P
<
0.05).
Cross-resistance
demonstrated,
with
a
3-fold
increase
in
IC50.
also
showed
reduced
0.05)
cells,
whereas
evaluated
remained
unchanged.
analysis
revealed
no
selective
although
influx/efflux
observed
across
all
models.
Conclusions:
In
vitro
simulation
patient
treatment
useful
establish
retinoblastomas
identify
strategies
overcome
resistance
through
drug
repurposed.
warrant
further
investigation
support
translation.
Anticancer Research,
Год журнала:
2024,
Номер
45(1), С. 73 - 79
Опубликована: Дек. 30, 2024
Background/Aim:
Organometallic
complexes
can
decrease
adhesion,
migration,
invasion
of
cancer
cells,
mainly
through
regulation
the
extracellular
matrix
and
therefore
act
against
metastases.
The
aim
was
to
investigate
anti-invasive
properties
a
rhenium-based
metal
compound,
rhenium(I)-diselenoether
(Re-diSe)
its
effects
on
metalloproteinase
MMP-2,
key
player
in
metastatic
processes,
cultured
MDA-MB231
triple-negative
breast
cells.
Materials
Methods:
Matrigel
utilized
assess
cell
adhesion
matrix.
Migration
capacity
evaluated
wound
healing
assay,
while
assays
were
conducted
using
transwell
assay
by
crystal
violet
dye.
MMP-2
levels
quantified
medium
fluorometric
kit
assay.
Cells
exposed
increasing
concentrations
drug
(5,
10,
20,
50
100
μM).
Three
time
points
exposure
cells
investigated
(48,
96
120
h).
Results:
Re-diSe
exhibited
effectiveness
even
at
doses
as
low
5
or
10
μM
all
biological
assays.
h
significantly
improved
effect
comparison
with
48
h.
Conclusion:
results
highlight
impact
