Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)
Опубликована: Сен. 11, 2024
Язык: Английский
Frontiers in Sustainable Food Systems, Год журнала: 2025, Номер 9
Опубликована: Март 26, 2025
With a growing global population, ways to counterbalance the demand for meat products with effective food security and waste management innovative scalable solutions. Concurrently, alarming incidence of end-stage organ failure, limited availability transplantable organs, directives reduce reliance on animal testing underscore need clinically viable sustainable alternatives. Our approach introduces hypothesis-driven, renewable tissue engineering strategy that creates low-cost keratoplasty models derived entirely from agri-food waste. Specifically, we hypothesize abundant by-products, such as eyes bladders, provide practically unlimited readily available supplies corneal tissues urine-derived stem cells (USCs) can be repurposed into cost-effective, relevant Traditional approaches often rely cadaveric tissues, invasive cell sourcing, or expensive commercial lines, which require complex resource-intensive processes, including high-end bioreactor systems manufacturing environments. These requirements limit widespread adoption technological progress needed increase supply keratografts. proposed leverages combination post-mortem bladder harvesting, in turn facilitates decellularization, non-invasive USC differentiation, compartment-specific recellularization help overcome barriers associated traditional seeding generate this type Overall, our perspective suggests way devise transformative resource-efficient engineering, specifically geared toward improving outcomes while offering broader applications regeneration other bodily tissues/organs biotechnological innovation.
Язык: Английский
Процитировано
1
Bioengineering, Год журнала: 2025, Номер 12(2), С. 120 - 120
Опубликована: Янв. 27, 2025
The management of segmental bone defects presents a complex reconstruction challenge for orthopedic surgeons. Current treatment options are limited by efficacy across the spectrum injury, morbidity, and cost. Regional gene therapy is promising tissue engineering strategy repair, as it allows local implantation nucleic acids or genetically modified cells to direct specific protein expression. In cell-based approaches, variety different cell types have been described including mesenchymal stem (MSCs) derived from multiple sources—bone marrow, adipose, skeletal muscle, umbilical cord tissue, among others. MSCs, in particular, well studied, they serve source osteoprogenitor addition providing vehicle transgene delivery. Furthermore, MSCs possess immunomodulatory properties, which may support development an allogeneic “off-the-shelf” product. Identifying optimal type paramount successful clinical translation approaches. Here, we review current strategies loss surgery, grafting, graft substitutes, operative techniques. We also highlight regional with focus on sources suitable this application.
Язык: Английский
Процитировано
0
Advances in experimental medicine and biology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
International Journal of Nanomedicine, Год журнала: 2025, Номер Volume 20, С. 1615 - 1634
Опубликована: Фев. 1, 2025
Background: Scar formation following large-area vaginal defects post-vaginoplasty is a major clinical challenge. Compared to skin scars, scars can lead pain during intercourse and urinary difficulties, severely impacting quality of life. Small extracellular vesicles (sEVs) encapsulate diverse bioactive components, making them potential therapeutic agents. Designing functional scaffolds that incorporate sEVs promising approach for scarless defect repair. Methods: sEVs-loaded were developed through electrostatic interactions between negatively charged secreted by urine-derived stem cells (USC-sEVs) positively human acellular amniotic membranes. The efficacy in the treatment rabbits was assessed histological analysis. Immunofluorescence staining, Western blot, qRT-PCR collagen gel contraction analyses conducted evaluate antifibrotic effects USC-sEVs. RNA sequencing employed elucidate underlying mechanisms involved. LC‒MS/MS analysis used identify candidate upstream proteins Results: In vivo experiments demonstrated promoted healing modulating deposition, reducing fibrosis, diminishing inflammation. vitro revealed USC-sEVs significantly inhibited proliferation, production, activation fibroblasts with fibrotic phenotype, indicating properties Transcriptome blot fibrosis downregulating TGF-β p38 MAPK signaling pathways. identified 2653 encapsulated decorin, an inhibitor pathway, DUSP3, negative regulator phosphorylation, enriched could be transferred fibroblasts. Conclusion: delivering decorin DUSP3 proteins, which regulate pathways, respectively. This study highlights as strategy repair vaginoplasty, offering novel regenerative medicine significant translational application. Keywords: small vesicles, cells, defects, repair, anti-fibrosis
Язык: Английский
Процитировано
0
Cell Death Discovery, Год журнала: 2025, Номер 11(1)
Опубликована: Апрель 15, 2025
Abstract Ataxia-telangiectasia (A-T) is a rare neurodegenerative disorder caused by the deficiency of serine/threonine kinase ataxia telangiectasia mutated (ATM) protein, whose loss function leads to altered cell cycle, apoptosis, oxidative stress balance and DNA repair after damage. The clinical manifestations are multisystemic, among them cerebellar degeneration muscular ataxia. molecular mechanism which ATM A-T still uncertain and, currently only symptomatic treatments available. In this study, we generated functional skeletal muscle model that recapitulates highlights role in calcium signalling contraction. To aim, using CRISPR/Cas9 technology, knocked out protein urine-derived stem cells (USCs) from healthy donors. resulting USCs-ATM-KO maintained stemness but showed G2/S cycle progression an inability UV Moreover, they increased cytosolic release ATP stimulation detriment mitochondria. alterations homoeostasis were differentiation into (USC-SkMCs) correlated with impaired Indeed, USC-SkMCs-ATM-KO contraction kinetics dramatically accelerated compared control cells. These results highlight relevant muscle, not dependent on non-functional neuronal communication, paving way for future studies interpretation
Язык: Английский
Процитировано
0
Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)
Опубликована: Сен. 11, 2024
Язык: Английский
Процитировано
1