Electroacupuncture and Tongbian decoction ameliorate CUMS-induced depression and constipation in mice via TPH2/5-HT pathway of the gut-brain axis

Brain Research Bulletin, Год журнала: 2025, Номер 221, С. 111207 - 111207

Опубликована: Янв. 14, 2025

Depression is commonly associated with gastrointestinal (GI) disorders, such as constipation, which can potentially intensify depressive symptoms. The interplay between these conditions believed to be facilitated by the gut-brain axis, suggests a complex bidirectional interaction. Current treatments, antidepressants and prokinetics, are often side effects high recurrence rates, highlighting need for effective treatments targeting both depression constipation. This study was designed assess therapeutic efficacy of electroacupuncture (EA) in conjunction Tongbian decoction (TB) management while also investigating underlying mechanisms through lens axis. Chronic unpredictable mild stress (CUMS) employed induce comorbidity model constipation mice, followed administration EA, EA + TB, fluoxetine (FLX). findings demonstrated that antidepressant combination were more pronounced than those alone. TB treatment significantly ameliorated anxiety-like behaviors, restored cognitive function, enhanced motility chronic mice. Furthermore, reduced intestinal inflammation, neuronal morphology, increased expression tryptophan hydroxylase 2 (TPH2) prefrontal cortex (PFC) colon, elevated serum levels 5-hydroxytryptophan (5-HTP)-a molecule acts connector-and promoted synthesis production serotonin (5-HT) tract brain. Contrastingly, FLX showed limited improving In conclusion, regulates TPH2/5-HT pathway via demonstrating synergistic regulation nervous systems, favorable prokinetic effects.

Язык: Английский

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Choline metabolism in ischemic stroke: An underappreciated "two-edged sword"

Pharmacological Research, Год журнала: 2025, Номер 214, С. 107685 - 107685

Опубликована: Март 5, 2025

Ischemic stroke (IS) is an important cause of death and disability worldwide, but the molecular mechanisms involved are not fully understood. In this context, choline metabolism plays increasingly role in IS due to its multifaceted involving neuroprotection, neuroregeneration, inflammatory response, immune regulation, long-term health effects. With deepening research on metabolites, such as trimethylamine-N-oxide (TMAO), scientists have gradually realized key occurrence, development potential treatment IS. This review summarizes importance neuroprotection disease management, highlighting complexity affecting cerebrovascular through gut microbes. Although metabolites exhibit a protective effect, excessive intake increases some may confer risk, suggesting need carefully balance dietary intake. The purpose integrate existing results provide theoretical basis for further exploring mechanism, prognosis evaluation clinical intervention ischemic IS, hoping new perspective enlightenment formulation effective prevention strategies, promote comprehensive understanding heart brain optimize methods.

Язык: Английский

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The dopamine receptor agonist rotigotine attenuated indomethacin-induced enteropathy in the small intestinal mucosa of mice

Molecular Biology Reports, Год журнала: 2025, Номер 52(1)

Опубликована: Март 24, 2025

Язык: Английский

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Electroacupuncture Attenuates Intestinal Barrier Disruption via the α7nAChR-Mediated HO-1/p38 MAPK/NF-κB Pathway in a Mouse Model of Metabolic Dysfunction-Associated Fatty Liver Disease: A Randomized, Single-Blind, Controlled Trial

Biomedicines, Год журнала: 2025, Номер 13(4), С. 802 - 802

Опубликована: Март 27, 2025

Background: Gut barrier integrity plays a crucial role in the pathogenesis of metabolic dysfunction-associated fatty liver disease (MAFLD). Electroacupuncture (EA) at ST-36 can ameliorate inflammatory responses via stimulating α7 nicotinic acetylcholine receptor (α7nAChR), but whether EA is effective preserving intestinal MAFLD has not been exactly illustrated. This investigation explored potential protection mechanisms targeting dismantled gut MAFLD. Methods: C57BL/6 mice were randomly allocated into several subgroups: control (CON), high-fat diet (HFD), HFD with EA, and α7nAChR inhibitor α-BGT, HO-1 knockout (KO). Body weight, visceral fat index, histopathological examination intestine determined. Serum biological indexes evaluated through corresponding kits. Furthermore, expressions HO-1, α7nAChR, barrier-associated proteins, molecular tissues assessed Western blot, RT-qPCR, immunohistology, or immunofluorescence examination. Results: treatment decreased body index gain mitigated function injury abnormal lipid indexes, exhibiting less severity hepatic steatosis, fibrosis, inflammation Lower permeability, epithelial disruption, upregulation tight junction proteins after suggested protective effects attenuating dysfunction. These abolished by α-BGT deletion. Mechanistically, markedly enriched expression phosphorylated p38 MAPK/NF-κB activation, which was lost KO treatment. Conclusions: The may be attributed to preserved barrier, thereby alleviating systemic preventing subsequent hits, where α7nAChR-mediated HO-1/p38 pathway maintain homeostasis.

Язык: Английский

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α7-Nicotinic Acetylcholine Receptor Activation Modulates BV2 Microglial Plasticity via miR-21/TNF-α/NFκB in Oxygen–Glucose Deprivation/Reoxygenation

Journal of Molecular Neuroscience, Год журнала: 2024, Номер 75(1)

Опубликована: Дек. 24, 2024

Язык: Английский

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