Structural characterization and anti-inflammatory activity of a neutral polysaccharide from Dendrobium huoshanense C. Z. Tang et S. J. Cheng

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 140339 - 140339

Опубликована: Янв. 1, 2025

Язык: Английский

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Immunometabolism of ferroptosis in the tumor microenvironment

Frontiers in Oncology, Год журнала: 2024, Номер 14

Опубликована: Авг. 12, 2024

Ferroptosis is an iron-dependent form of cell death that results from excess lipid peroxidation in cellular membranes. Within the last decade, physiological and pathological roles for ferroptosis have been uncovered autoimmune diseases, inflammatory conditions, infection, cancer biology. Excitingly, metabolism may be targeted to induce by cancers are resistant other forms death. sensitivity regulated oxidative stress, metabolism, iron which all influenced tumor microenvironment (TME). Whereas some types shown adapt these stressors, it not clear how immune cells regulate their sensitivities ferroptosis. In this review, we discuss mechanisms different subsets, influences infiltrate TME, interactions can determine epithelial-to-mesenchymal transition (EMT) metastasis. While much focus has placed on inducing cells, important considerations ferroptosis-modulating strategies impact anti-tumor immunity. From perspective, also promising immunotherapies field challenges associated with targeting specific populations.

Язык: Английский

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Skin Telocytes Could Fundament the Cellular Mechanisms of Wound Healing in Platelet-Rich Plasma Administration

Cells, Год журнала: 2024, Номер 13(16), С. 1321 - 1321

Опубликована: Авг. 8, 2024

For more than 40 years, autologous platelet concentrates have been used in clinical medicine. Since the first formula used, namely platelet-rich plasma (PRP), other experimented with, including fibrin and concentrated growth factor. Platelet three standard characteristics: they act as scaffolds, serve a source of factors cytokines, contain live cells. PRP has become extensively regenerative medicine for successful treatment variety (non-)dermatological conditions like alopecies, acne scars, skin burns, ulcers, muscle, cartilage, bone repair, an adjuvant post-surgery wound healing, with obvious benefits terms functionality aesthetic recovery affected tissues/organs. These indications were well documented, large amount evidence already published supporting efficacy this method. The primordial principle behind minimally invasive treatments is usage patient’s own platelets. transplantation thrombocytes are significant, representing fast economic method that requires only basic equipment training, it biocompatible, thus being low risk patient (infection immunological reactions can be virtually disregarded). Usually, structural applying attributed to fibroblasts only, considered most numerous cell population within interstitium. However, apparent simplistic explanation still eluding those different types interstitial cells (distinct from fibroblasts) residing stromal tissue, e.g., telocytes (TCs). Moreover, dermal TCs documented potential angiogenesis (extra-cutaneous, but also skin), their implication few dermatological was attested described ultrastructurally immunophenotypically. Interestingly, biochemically consists series factors, molecules, which proven positive expression. Thus, attractive hypothesize document any tissular collaboration between cutaneous administered local recovery/repair/regeneration. Therefore, could perceived missing link necessary provide solid good results achieved by administering skin-repairing processes.

Язык: Английский

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Integrins and Integrin-Driven Secretory Pathways as Multi-dimensional Regulators of Tumor-Associated Macrophage Recruitment and Reprogramming in Tumor Microenvironment

Matrix Biology, Год журнала: 2024, Номер 135, С. 55 - 69

Опубликована: Дек. 5, 2024

Язык: Английский

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The Anti‐Inflammatory Activities of Sphagnum palustre L. Ethanol Extract to Control Inflammation in RAW264.7 Cells

Flavour and Fragrance Journal, Год журнала: 2025, Номер unknown

Опубликована: Янв. 15, 2025

ABSTRACT Discomfort caused by inflammation leads to stress and anxiety in patients seriously decreases the patients' quality of life. People prefer use natural products instead anti‐inflammatory drugs because their low toxicity side effects. Studies have shown that Sphagnum palustre L. ( S. ) can be used as medicinal plant, but few studies focused on its This study explored mechanism action ethanol extract peat moss plant lipopolysaccharide‐induced macrophage RAW264.7 cells. Components extracts (SPE) were identified HPLC‐MS, which mainly included 4‐methoxybenzaldehyde, 4‐methoxycinnamaldehyde oleanolic acid. The effects different concentrations (6.25–100 μg/mL) SPE after 24 h administration evaluated establish a cellular model. Three biological replicates performed based each experiment, MTT assay results showed concentration promoted cell proliferation marked Formazan. In neutral red uptake assay, group was effectively inhibited phagocytosis rate. With increase concentration, intracellular ROS release decreased, detected DCFH‐DA. Immunofluorescence result reactive oxygen species from macrophages with fluorescent markers DAPI. nitric oxide well. What's more, significantly decreased protein expression interleukin (IL)‐1, IL‐6, nuclear factor (NF)‐κB according enzyme‐linked immunosorbent immunocytochemical assays. reduces cells thus is promising plant.

Язык: Английский

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Association between pan-immune-inflammation value and heart failure: Evidence from the NHANES 2011–2020

Journal of International Medical Research, Год журнала: 2025, Номер 53(3)

Опубликована: Март 1, 2025

Objective This study aimed to explore the association between pan-immune-inflammation value and heart failure outcomes using data from nationally representative National Health Nutrition Examination Survey database. Methods We conduct a cross-sectional cohort analysis data, including participants aged ≥20 years with available data. performed smooth curve fitting threshold analysis, both linear non-linear regression models, assess dose–response relationship continuous effect of on outcomes. Results Our revealed significant independent elevated levels an increased risk occurrence. After adjustment for multiple covariates, these findings remained consistent each increment unit in logarithmic is associated 34% increase Furthermore, we identified inflection point = 5.98 as critical threshold. Stratified analyses that occurrence remains across different subgroups. Conclusions confirmed clinical novel inflammatory biomarker assessment monitoring failure.

Язык: Английский

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Protocol for the generation and assessment of functional macrophages from mouse bone marrow cells

STAR Protocols, Год журнала: 2025, Номер 6(2), С. 103706 - 103706

Опубликована: Март 21, 2025

Язык: Английский

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Novel function of macrophage migration inhibitory factor in regulating post-infarct inflammation and the therapeutic significance

Journal of Advanced Research, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

Recent studies indicate that macrophage migration inhibitory factor (MIF) has a dual role in myocardial infarction (MI), with different cellular sources of MIF influencing inflammation and healing differentially. To investigate the underlying mechanism MI interventional efficacy targeting MIF. Wild-type (WT), global gene knockout (KO) chimeric mice were subjected to coronary artery occlusion. The inflammatory responses processes following studied both vivo vitro settings. Furthermore, therapeutic potential pharmacological inhibition improve prognosis was explored. Globally, enhanced systemic local responses, as well splenic monocyte mobilization, MI. promoted through CCR2 CXCR4 peripheral blood mononuclear cells (PBMCs) infarcted myocardium. Additionally, augmented angiotensin Ⅱ type 1 receptor (AT-1R) expression interacted AT-1R promote mobilization acute derived from bone marrow (KOWT mice) had stronger monocytes. In contrast, deficiency leukocytes (WTKO increased Ly-6Clow accumulation, M2 infiltration, degree fibrosis vitro, ischemic heart but impaired M1 marker PBMCs. Anti-MIF treatment effectively attenuated regional post-MI without affecting process, thereby improving long-term prognosis. Deletion inflammatory-cell-derived diminished by inhibiting downregulating pro-inflammatory mediators, while cardiac-derived exerted anti-inflammatory influence facilitated healing. antibody therapy protected severe injury improved

Язык: Английский

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IgG4-mediated M2 macrophage polarization in tertiary lymphoid structures of esophageal cancer: implications for immunosuppression

Frontiers in Immunology, Год журнала: 2025, Номер 15

Опубликована: Янв. 17, 2025

Our previous research highlighted the potential role of immunoglobulin G4 (IgG4) in mediating immunosuppression within tumor microenvironment (TME). Tertiary lymphoid structures (TLS) TME have important immune-related functions. This study aims to analyze distribution characteristics IgG4-expressing cells, regulatory T cells (Tregs), and M2-type macrophages as well elucidate relationship between IgG4 polarization M2 TLS esophageal cancer. To IgG4, Treg assess impact on macrophage polarization. Esophageal cancer tissue were analyzed with multiplex immunofluorescence determine spatial density B their subtypes. The CD8+ TLS, along interleukin-10 (IL-10) expression presence, was studied. Serum IL-10 levels compared patients healthy controls. In vitro, monocyte differentiation into observed. inversely related mature indicating a immunosuppressive (P<0.05,*). analysis revealed that both (P<0.01, **) (P<0.0001, ****) significantly elevated positively correlated controls **). vitro experiments confirmed macrophages, potentially enhancing phenotype TLS. may contribute by promoting which could be therapeutic target.

Язык: Английский

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The Relationship Between the Monocyte-to-High-Density Lipoprotein Cholesterol Ratio and Platelet Volume Indices With Diabetic Retinopathy

Cureus, Год журнала: 2025, Номер unknown

Опубликована: Фев. 28, 2025

Aim This study aims to evaluate the roles of monocyte count-to-high-density lipoprotein (HDL) cholesterol ratio (MHR) and platelet volume indices (PVI) in predicting diabetic retinopathy (DR) patients diagnosed with type 2 diabetes mellitus (DM) explore new methods for early prediction retinopathy. Methods prospective included 120 aged over 18 years DM according American Diabetes Association criteria, along a control group 40 healthy individuals. Patients were divided into three groups: without retinopathy, non-proliferative (NPDR), proliferative (PDR). Parameters such as complete blood count, PVI (mean volume, distribution width, plateletcrit, large cell ratio), lipid profile, inflammatory markers (sedimentation rate, C-reactive protein), MHR, renal function tests, glucose metabolism analyzed alongside demographic, clinical, laboratory data assess their associations DR both patient groups. Results investigated relationship between hematological parameters (PVI, MHR). Hypertension, hyperlipidemia, chronic kidney disease common comorbidities. NPDR more frequently used metformin dipeptidyl peptidase (DPP)-4 inhibitors, while PDR often insulin (p<0.05). Diabetic neuropathy nephropathy prevalent group. had significantly longer duration. Mean (MPV) (PLCR) higher ROC analysis identified MHR (>11.9, 65% sensitivity, 35% specificity) plateletcrit (PCT<0.29%, 61% 80% cut-offs prediction. MHR's area under curve (AUC) was greater than PCT's MPV differed NPDR, but did not differ among groups or DR/non-DR Glycosylated hemoglobin (HbA1c) duration correlated positively which also leukocyte count. HbA1c width (PDW). Each unit increase increased 1.13-fold, each 1.9-fold. Conclusions The compared those PLCR levels patients. In evaluating significant cut-off values PCT found be predictive indicators

Язык: Английский

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