Dysregulated key long non-coding RNAs TP53TG1, RFPL1S, DLEU1, and HCG4 associated with epithelial-mesenchymal transition (EMT) in castration-resistant prostate cancer

Advances in Cancer Biology - Metastasis, Год журнала: 2025, Номер unknown, С. 100132 - 100132

Опубликована: Янв. 1, 2025

Язык: Английский

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Development of a ferroptosis-related gene prognostic model and molecular subgroups characterization in sepsis

Molecular Immunology, Год журнала: 2025, Номер 178, С. 1 - 11

Опубликована: Янв. 6, 2025

Язык: Английский

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CircPRKCA facilitates esophageal squamous cell carcinoma metastasis via m5C-dependent CSF2 mRNA stabilization

Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)

Опубликована: Апрель 1, 2025

Esophageal squamous cell carcinoma (ESCC) is a serious invasive malignancy with an ambiguous etiology. Evidence indicates that circular RNA (circRNA) significantly involved in the regulatory processes associated cancer development. Nevertheless, specific molecular mechanisms through which circRNA facilitates progression of ESCC are still largely undefined. Here, we identified expression hsa_circ_0007580 (designated circPRKCA) was markedly elevated ESCC. Fluorescence situ hybridization (FISH) conducted to verify expression, intracellular localization, and potential prognostic value circPRKCA based on tissue microarray. Gain- loss-of-function assays were employed investigate effects both vitro vivo. pull-down mass spectrometry (MS) performed identify proteins bound circPRKCA. mRNA sequencing screen downstream target genes Furthermore, immunoprecipitation methylated (MeRIP) analysis used explore mechanisms. We found exhibited significant upregulation tissues correlated unfavorable outcomes. Biological function experiments further confirmed enhances capabilities migration, invasion, angiogenesis Mechanistically, engages interaction Y-box binding protein 1 (YBX1) within cytoplasmic milieu, consequently preventing ubiquitination-mediated degradation YBX1. Increased concentrations YBX1 increase stability granulocyte–macrophage colony-stimulating factor (CSF2) 5-methylcytosine (m5C)-dependent manner. This process metastasis In this research, correlation between prognoses patients It instrumental metastatic via YBX1/CSF2 signaling pathway. Consequently, targeting may represent promising therapeutic strategy for

Язык: Английский

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Identification of potential immune-related genes and infiltrations in temporomandibular joint osteoarthritis

Annals of Medicine and Surgery, Год журнала: 2024, Номер 86(12), С. 7135 - 7146

Опубликована: Окт. 22, 2024

Objective: The aim of this study was to investigate the potential inflammatory cytokines and chemokines markers for temporomandibular joint osteoarthritis (TMJOA) diagnosis using a bioinformatics analysis. Methods: differentially expressed genes mRNA (DEGs) transcripts lncRNA (DETs) were identified between TMJOA samples normal controls curated from GSE205389 by “DESeq. 2” R package. KEGG GO conducted package “ggplot2” “clusterProfiler”. A PPI network constructed identify hub STRING Cytoscape. co-expression check regulation function on protein-coding genes. Finally, immune cell infiltration analysis with CIBERSORTx confirmed xCells. Results: authors 171 DEGs DETs, which closely related response, T-cell activation, cytokine-cytokine-receptor interaction, muscle system process. screened top 10 genes, including IL6, IL1B, IL10, CCL2, CCL5, CXCL1, CXCL10, ICAM1, CSF1 MMP1 . Additionally, showed that CD8 + T cells, M1 macrophage B cells increased in samples. demonstrated mainly enriched muscle-related pathways. Conclusions: found system-related pathways as well played significant role development. could be crucial early-stage personalized treatment strategies.

Язык: Английский

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AEBP1 Silencing Protects Against Cerebral Ischemia/Reperfusion Injury by Regulating Neuron Ferroptosis and Microglia M2 Polarization Through PRKCA‐PI3K‐Akt Axis

Drug Development Research, Год журнала: 2024, Номер 85(8)

Опубликована: Дек. 1, 2024

ABSTRACT Cerebral ischemia/reperfusion injury is one of the main causes neuronal damage. Neuron ferroptosis and microglia polarization are considered as critical processes during cerebral ischemia/reperfusion. Adipocyte enhancer‐binding protein 1 (AEBP1) usually acts a transcriptional repressor which involved in various diseases. However, it still remains unknown whether AEBP1 could have important roles regulating neuron injury. The oxygen‐glucose deprivation reperfusion (OGD/R)‐treated cells middle artery occlusion (MCAO)‐treated mice were used vitro vivo models. differentially expressed factors analyzed according to GEO datasets. Relative mRNA expression levels detected by qRT‐PCR western blot analysis. Cell viability was measured CCK‐8 assay. ROS, GSH iron contents using specifical assay kits. CD26 CD206 immunofluorescence Inflammatory cytokines ELISA. association between PRKCA assessed luciferase reporter ChIP analyses. damage TTC staining neurological deficit score. Transcription factor increased OGD/R‐treated HT22 BV2 cells. silencing attenuated OGD/R‐induced cell through increasing viability, GPX4 levels, decreasing ACSL4 levels. knockdown promoted M2 CD206‐positive Arg‐1 level, reducing iNOS, TNF‐α, IL‐1β IL‐6 transcriptionally repressed expression, further regulated PI3K/Akt signaling activation. Inhibition or reversed effects on polarization. downregulation infarct size scores MCAO‐treated mice. mitigated activating signaling, indicating potentially protective action

Язык: Английский

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Dual-specificity phosphatase 23 functions as a promising prognostic biomarker in non-small cell lung cancer

Genes & Genomics, Год журнала: 2024, Номер unknown

Опубликована: Дек. 18, 2024

Язык: Английский

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