Development of a SYBR Green-Based Real-Time PCR Assay to Detect Oncomelania hupensis quadrasi DNA in Environmental Water Samples DOI Creative Commons
Daria L. Manalo, Jude Karlo G. Bolivar,

Karl Ian T. Ermino

и другие.

Tropical Medicine and Infectious Disease, Год журнала: 2025, Номер 10(5), С. 140 - 140

Опубликована: Май 20, 2025

Oncomelania hupensis quadrasi is the intermediate host of S. japonicum, causative species schistosomiasis in Philippines. Conventionally, risk areas are identified by procedures requiring highly skilled personnel and constant surveillance efforts. Recent developments disease diagnostics explore utilization environmental DNA as targets for polymerase chain reactions surveillance. In this study, a low-cost, specific, efficient SYBR Green-based real-time PCR assay to detect O. h. from water samples was developed, optimized, validated. Primers were designed based on A18 microsatellite region quadrasi. The exhibited detection limit one copy number per microliter at 99.4% efficiency R2 = 0.999, which specifically amplified only. Validation demonstrated 100% PPV NPV values, suggesting its potential tool identifying areas, pathogen-directed surveillance, policy making, control.

Язык: Английский

Development of a SYBR Green-Based Real-Time PCR Assay to Detect Oncomelania hupensis quadrasi DNA in Environmental Water Samples DOI Creative Commons
Daria L. Manalo, Jude Karlo G. Bolivar,

Karl Ian T. Ermino

и другие.

Tropical Medicine and Infectious Disease, Год журнала: 2025, Номер 10(5), С. 140 - 140

Опубликована: Май 20, 2025

Oncomelania hupensis quadrasi is the intermediate host of S. japonicum, causative species schistosomiasis in Philippines. Conventionally, risk areas are identified by procedures requiring highly skilled personnel and constant surveillance efforts. Recent developments disease diagnostics explore utilization environmental DNA as targets for polymerase chain reactions surveillance. In this study, a low-cost, specific, efficient SYBR Green-based real-time PCR assay to detect O. h. from water samples was developed, optimized, validated. Primers were designed based on A18 microsatellite region quadrasi. The exhibited detection limit one copy number per microliter at 99.4% efficiency R2 = 0.999, which specifically amplified only. Validation demonstrated 100% PPV NPV values, suggesting its potential tool identifying areas, pathogen-directed surveillance, policy making, control.

Язык: Английский

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