Decoding multiple myeloma: single-cell insights into tumor heterogeneity, immune dynamics, and disease progression DOI Creative Commons

Zhenzhen Zhao,

Zhijie Zhao, Zhiheng Lin

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Май 8, 2025

Background Multiple myeloma (MM) is a biologically heterogeneous malignancy of clonal plasma cells, often progressing from MGUS or smoldering MM. It causes anemia, bone lesions, and immune dysfunction due to abnormal cell expansion in the marrow. Neuroinflammatory neurotrophic factors may influence MM progression by affecting cells marrow niche. Growing evidence points role for neuroimmune regulation tumor immunity. Despite therapeutic progress, disease heterogeneity resistance highlight need new strategies targeting microenvironment axis. Methods This investigation exploited single-cell RNA sequencing (scRNA-seq) analyze high-risk multiple (SMMh) samples, identifying 11 distinct types. We examined their transcriptional signatures, stemness, proliferative properties, metabolic pathways, with particular attention interactions microenvironment. Using trajectory inference tools such as CytoTRACE, Monocle2, Slingshot, we traced differentiation paths subpopulations identified key signaling pathways that responses progression. Results The analysis four C0 IGLC3+ representing least differentiated most subset. These played critical contribute evasion mechanisms. Additionally, receptor-ligand within were identified, which be influenced neuroinflammatory factors. findings suggest nervous system modulation significantly affect biology, highlighting potential targets could overcome conventional therapies. Conclusion provided insights into cellular diversity trajectories MM, offering deeper understanding complex drive resistance. By incorporating neuroinflammation modulation, our study suggested novel axis oncology, ultimately contributing development more effective, personalized treatment approaches

Язык: Английский

MAZ-mediated tumor progression and immune evasion in hormone receptor-positive breast cancer: Targeting tumor microenvironment and PCLAF+ subtype-specific therapy DOI
Gaofeng Ni,

Yuwei Sun,

Hongling Jia

и другие.

Translational Oncology, Год журнала: 2025, Номер 52, С. 102280 - 102280

Опубликована: Янв. 13, 2025

Язык: Английский

Процитировано

6
Single-cell RNA sequencing and immune microenvironment analysis reveal PLOD2-driven malignant transformation in cervical cancer DOI Creative Commons
Zhiheng Lin,

Fengxin Wang,

Renwu Yin

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 15

Опубликована: Янв. 7, 2025

Cervical cancer is the fourth most common in women globally, and main cause of disease has been found to be ongoing HPV infection. remains primary cancer-related death despite major improvements screening treatment approaches, especially low- middle-income nations. Therefore, it crucial investigate tumor microenvironment advanced cervical order identify possible targets. In better understand malignant epithelial cells (EPCs), this study used bulk RNA-seq data from UCSC conjunction with single-cell RNA sequencing ArrayExpress database. After putting quality control procedures into place, cell type identification clustering analysis using Seurat software were carried out. To clarify functional pathways, enrichment differential gene expression The CIBERSORT ESTIMATE R packages evaluate immune characteristics, univariate multivariate Cox regression analyses extract prognostic features. Furthermore, assessments drug sensitivity Eight types identified, EPCs showing high proliferative stemness Five EPC subpopulations defined, C1 NNMT+ CAEPCs driving differentiation. A NNMT Risk Score (NCRS) model was developed, revealing a correlation between elevated NCRS scores adverse patient outcomes characterized by evasion. vitro experiments validated that PLOD2 significantly enhances proliferation, migration, invasion cells. This investigation delineated eight five cancer, establishing as therapeutic target. demonstrated its capability, indicating higher are associated poorer clinical outcomes. validation highlights potential, underscoring critical need for integrating immunotherapy targeted strategies enhance diagnostic approaches cancer.

Язык: Английский

Процитировано

5
Inhibition of programmed cell death by melanoma cell subpopulations reveals mechanisms of melanoma metastasis and potential therapeutic targets DOI Creative Commons
Yuepeng An,

Fu Zhao,

Hongling Jia

и другие.

Discover Oncology, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 20, 2025

Melanoma is an aggressive type of skin cancer that arises from melanocytes, the cells responsible for producing pigment. In contrast to non-melanoma cancers like basal cell carcinoma and squamous carcinoma, melanoma more invasive. was distinguished by its rapid progression, high metastatic potential, significant resistance conventional therapies. Although it accounted a small proportion cases, accounts majority deaths caused due ability invade deep tissues, adapt diverse microenvironments, evade immune responses. These unique features highlighted challenges treating underscored importance advanced tools, such as single-cell sequencing, unravel biology develop personalized therapeutic strategies. Thus, we conducted analysis cellular composition within tumor tissues further subdivided into subpopulations. Through analyzing metabolic pathways, stemness genes, transcription factors (TFs) among in different phases (G1, G2/M, S) well between primary foci cells, investigated specific mechanisms underlying metastasis. We also revisited temporal trajectories subpopulations, identifying core subpopulation C0 SOD3 + cells. Our findings revealed close relationship pivotal oxidative pathways tissues. Additionally, analyzed prognostically relevant differentially expressed genes (DEGs) built predictive model associated with outcomes. selected gene IGF1 highest coefficient (coef) value analysis, experimentally validated essential function proliferation invasive metastasis melanoma. infiltration discovered critical roles played M1/M2 macrophages progression evasion. Furthermore, development malignant were closely various forms programmed death (PCD), including apoptosis, autophagic death, ferroptosis, pyroptosis. often resisted mechanisms, maintaining their growth inhibiting apoptosis evading death. Meanwhile, induction ferroptosis pyroptosis thought trigger responses helped suppress dissemination. A deeper understanding PCD provided foundation developing novel targeted therapies, potential enhance treatment efficacy. contributed prognostic models shed light on research directions concerning targets.

Язык: Английский

Процитировано

0
Discovering the Potential Role of the C2 DUSP2+ MCs Subgroup in Lung Adenocarcinoma DOI
Shengyi Zhang,

Xinhan Li,

Zhikai Xiahou

и другие.

Translational Oncology, Год журнала: 2025, Номер 54, С. 102295 - 102295

Опубликована: Фев. 26, 2025

Язык: Английский

Процитировано

0
Exposing the cellular situation: findings from single cell RNA sequencing in breast cancer DOI Creative Commons
Gaofeng Ni,

Xinhan Li,

Wenyang Nie

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Март 6, 2025

Breast Cancer (BC) ranks among the top three most prevalent cancers globally and stands as principal contributor to cancer-related fatalities women. In spite of substantial occurrence rate BC, early stage this disease is generally regarded curable. However, intra-tumor heterogeneity presents a formidable obstacle success effective treatment. research, single cell RNA sequencing was utilized dissect tumor microenvironment within BC. Slingshot, CytoTRACE Monocle 2 were applied illustrate differentiation process each subpopulation in pseudotime sequence. To comprehensively comprehend cells (TCs) an analysis upstream transcription factors carried out via pySCENIC, while downstream pathway enrichment conducted through KEGG, GO GSEA. The prognosis model established based on bulk data obtained from TCGA GEO databases. Knock-down experiments also implemented explore function factor CEBPD TCs. Our in-depth identified eight types. Notably, TCs predominantly found epithelial cells. classification further uncovered five unique subpopulations, with one characterized by high UGDH expression. This shown possess distinct metabolic features metabolism-related investigations. intricate communication modalities different types effectively demonstrated means CellChat. Additionally, crucial factor, CEBPD, identified, which pronounced propensity towards tumors harbored potential tumor-advancing characteristics. Its role promoting cancer subsequently verified vitro knock-down experiments. Moreover, prognostic developed, risk score genes incorporated model. Through comparing prognoses UTRS levels, it determined that group had less favorable prognosis. These outcomes contributed elucidation complex interrelationships BC microenvironment. By specifically targeting certain subpopulations TCs, novel treatment strategies could potentially be devised. study shed light direction future research should take, furnishing valuable information can enhance regimens.

Язык: Английский

Процитировано

0
Single-cell insights into HNSCC tumor heterogeneity and programmed cell death pathways DOI

Yuanhao Chai,

Jianlin Zhang, Wenwen Shao

и другие.

Translational Oncology, Год журнала: 2025, Номер 54, С. 102341 - 102341

Опубликована: Март 10, 2025

Язык: Английский

Процитировано

0
The Potential Role of C4 MYH11+ Fibroblasts and the MDK-SDC2 Ligand-Receptor Pair in Lung Adenocarcinoma: Implications for Prognosis and Therapeutic Strategies DOI

H. Jia,

Yanjie Bian, Jie Yuan

и другие.

Translational Oncology, Год журнала: 2025, Номер 55, С. 102364 - 102364

Опубликована: Март 22, 2025

Язык: Английский

Процитировано

0
Investigation of the role of GEM in systemic lupus erythematosus through multi-omics joint analysis DOI Creative Commons
Ruofei Chen, Xiao Zhang,

Yifang Shang

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Апрель 9, 2025

Systemic lupus erythematosus (SLE) is a persistent autoimmune disorder marked by dysregulation of the immune system, resulting in extensive tissue inflammation and subsequent damage. Fibroblasts are essential contributors to pathogenesis SLE, particularly driving progression fibrosis inflammation. Recent research has proposed that GEM gene may regulate fibroblast activity SLE. However, precise molecular mechanisms through which modulates functions context SLE yet be fully elucidated. Gaining insight into these crucial for uncovering potential therapeutic targets aimed at addressing associated with Single-cell RNA sequencing was integrated cell-based assays, such as quantitative reverse transcription PCR (qRT-PCR) functional cellular experiments, investigate underlying mechanisms. The regulatory fibroblasts were analyzed cell assays. Differential expression subpopulations identified single-cell sequencing, emerging key implicated alterations. Trajectory analysis indicated correlated proliferation migration. Subsequent experiments confirmed regulates viability influences disease modulation proliferation, migration, apoptosis. highly differentially expressed within its altered impacts apoptosis, potentially contributing

Язык: Английский

Процитировано

0
Single-cell RNA sequencing in diffuse large B-cell lymphoma: tumor heterogeneity, microenvironment, resistance, and prognostic markers DOI Creative Commons
Linwei Li,

Qiwei Li,

Rui Niu

и другие.

Frontiers in Oncology, Год журнала: 2025, Номер 15

Опубликована: Апрель 9, 2025

Diffuse large B-cell lymphoma (DLBCL) is a highly heterogeneous malignancy with challenges in treatment resistance and relapse. Single-cell RNA sequencing (scRNA-seq) has provided important insights into tumor heterogeneity, microenvironment interactions, mechanisms, prognostic biomarkers. This review summarizes key findings from scRNA-seq studies, which have deepened our understanding of DLBCL contributed to the development precision therapeutic strategies. Integrating spatial transcriptomics single-cell multi-omics may further elucidate disease mechanisms identify novel targets, supporting advancement medicine DLBCL.

Язык: Английский

Процитировано

0
Immunotherapeutic strategies for invasive bladder cancer: a comprehensive review DOI Creative Commons
Yingying Wang, Min He, Jian Li

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Апрель 30, 2025

Bladder cancer is a prevalent malignancy, with muscle-invasive bladder (MIBC) presenting significant therapeutic challenge. Standard treatments, including radical cystectomy (RC) and neoadjuvant chemotherapy, pose substantial risks impact quality of life, leading to increasing interest in bladder-preserving therapies (BPT). Immunotherapy has revolutionized management, strategies ranging from intravesical Bacillus Calmette-Guérin (BCG) immune checkpoint inhibitors targeting programmed cell death protein 1 (PD-1) its ligand (PD-L1). In BCG-unresponsive non-muscle-invasive (NMIBC), PD-1 such as pembrolizumab offer promising response rates. MIBC, immunotherapy agents like atezolizumab improves pathological complete (pCR) facilitates preservation. Combination regimens integrating radiotherapy, not only enhance treatment efficacy but also exploit mechanisms immunogenic antigen release that further augment antitumor responses. This review provides comprehensive analysis current immunotherapeutic for invasive cancer, highlighting their clinical applications future potential.

Язык: Английский

Процитировано

0