Synthesis, characterization, and evaluation of copper-doped zinc oxide nanoparticles anticancer effects: in vitro and in vivo experiments

BMC Cancer, Год журнала: 2025, Номер 25(1)

Опубликована: Янв. 8, 2025

Zinc oxide and copper nanoparticles are known for their promising biological activities. This study aims to synthesize zinc copper-doped harness the combined cytotoxic anticancer effects of them in vitro vivo studies. nanoparticles, both doped undoped, were synthesized using a chemical co-precipitation method. All synthetized examined shape, crystal structure morphology/ microstructure X-ray diffractometers, scanning electron microscopy transmission microscopy. The hydrodynamic diameter zeta-potential was measured by dynamic light scattering. Energy Dispersive Spectroscopy evaluated doping nanoparticles. tested on bone cancer fibroblast cells normal lung cell viability test, colony formation assay, lactate dehydrogenase assay at concentrations 0, 1, 10, 17.5, 25, 50, 100, 200 μg/ml. In experiments assessed serum markers (Aspartate aminotransferase, Alanine transaminase, blood urea nitrogen creatinine) liver malondialdehyde levels response 5 mg/kg 50 doses. exhibited spherical morphology good dispersion, with an average grain size ranging from 15–39 nm. Copper-doped displayed mixture rod-like grain-like structures, larger 18–68 diffraction analysis confirmed wurtzite types While individual sizes varied, mean particle all samples, including those increasing doping, approximately 100 ± 0.1 Both negative zeta potential. studies revealed that bulk activity (cell < 80%) induced apoptosis 17.5 μg/ml after 72 h (P 0.05). demonstrated higher cytotoxicity compared also showed significant inhibition proliferation over 10 days indicated no changes Aspartate nitrogen, creatinine mg/kg. However, dose significantly increased these Histological injury rats treated but not 0.5 exhibit enhanced oxide, particularly concentrations. High doses could induce biochemical vivo, highlighting need careful management.

Язык: Английский

Impact of Cu1-xNixO on the structural, optical and photoluminescence performance of nano Zn0.95Mg0.05O/Cu1-xNixO

Applied Physics A, Год журнала: 2025, Номер 131(6)

Опубликована: Май 8, 2025

Язык: Английский