Spermatogenic cell apoptosis and impaired spermatogenesis in prepubertal mice: time- and dose-dependent toxicity of silver nanoparticles DOI

Zannatul Maowa,

Md Rafiur Rahman,

M. Nazmul Hoque

и другие.

Reproduction Fertility and Development, Год журнала: 2025, Номер 37(8)

Опубликована: Апрель 29, 2025

Context The increasing use of nanoparticles (NPs) in various consumer, agricultural, and pharmaceutical applications has raised considerable concern about their potential risks to human health the environment. Aims This study investigated progressive toxic effects silver (AgNPs) mouse testes after single repeated exposure. Methods Prepubertal male mice were exposed AgNPs by gavage at 50, 200, 500 mg/kg body weight. Testis, epididymis, serum collected subjected histopathological analysis. Key results Daily exposure for 7 15 days (n = 8) decreased sperm count, while abnormal count testicular atrophy a dose- exposure-time-dependent manner. A dose 200 weight resulted changes spermatogenic cell apoptosis time-dependent highest number apoptotic cells was detected 24 h exposure, whereas testosterone (TT) concentrations 12 h. To explore whether suppress TT affecting hypothalamus–pituitary–testicular (HPT) axis, we analyzed LH concentrations; however, no significant levels found. Conclusion showed that cause adverse on testis, specifically, apoptosis, impaired spermatogenesis an time- dose-dependent toxicity not associated with suppression HPT possibly involving other mechanisms. Implications These findings contribute broader discussion NP safety regulatory considerations, particularly regarding reproductive toxicity.

Язык: Английский

Spermatogenic cell apoptosis and impaired spermatogenesis in prepubertal mice: time- and dose-dependent toxicity of silver nanoparticles DOI

Zannatul Maowa,

Md Rafiur Rahman,

M. Nazmul Hoque

и другие.

Reproduction Fertility and Development, Год журнала: 2025, Номер 37(8)

Опубликована: Апрель 29, 2025

Context The increasing use of nanoparticles (NPs) in various consumer, agricultural, and pharmaceutical applications has raised considerable concern about their potential risks to human health the environment. Aims This study investigated progressive toxic effects silver (AgNPs) mouse testes after single repeated exposure. Methods Prepubertal male mice were exposed AgNPs by gavage at 50, 200, 500 mg/kg body weight. Testis, epididymis, serum collected subjected histopathological analysis. Key results Daily exposure for 7 15 days (n = 8) decreased sperm count, while abnormal count testicular atrophy a dose- exposure-time-dependent manner. A dose 200 weight resulted changes spermatogenic cell apoptosis time-dependent highest number apoptotic cells was detected 24 h exposure, whereas testosterone (TT) concentrations 12 h. To explore whether suppress TT affecting hypothalamus–pituitary–testicular (HPT) axis, we analyzed LH concentrations; however, no significant levels found. Conclusion showed that cause adverse on testis, specifically, apoptosis, impaired spermatogenesis an time- dose-dependent toxicity not associated with suppression HPT possibly involving other mechanisms. Implications These findings contribute broader discussion NP safety regulatory considerations, particularly regarding reproductive toxicity.

Язык: Английский

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