Eco-friendly Nanoparticle Phytosynthesis via Plant Extracts: Mechanistic Insights, Recent Advances, and Multifaceted Uses
Nano TransMed,
Год журнала:
2025,
Номер
unknown, С. 100080 - 100080
Опубликована: Март 1, 2025
Язык: Английский
Chitosan Nanoparticles as a Strategy for enhancing the Tropane Alkaloid Compounds Production and h6h Gene Expression in Hyoscyamus reticulatus Hairy Roots
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Март 14, 2025
Abstract
Background:
Hyoscyamus
reticulatus
L.
is
a
rich
source
of
tropane
alkaloids
(TA)
which
widely
used
in
medicine.
In
this
study,
hairy
roots
were
obtained
from
two-week
cotyledon
explants
H.
by
using
the
A7
strain
Agrobacterium
rhizogenes.
The
effects
different
concentrations
chitosan
nanoparticles
(CNP)
(0,
10,
30,
50
and
100
mg
L−1)
as
signaling
molecule
with
two
exposure
times
(24
48
h)
on
growth
rate,
antioxidant
enzymes
activity,
phenol
content,
TA
contents
hyoscyamine-6-beta-hydroxylase
(h6h)
gene
expression
levels
investigated.
After
extraction
roots,
High-performance
liquid
chromatography
(HPLC)
was
to
measure
amount
hyoscyamine
scopolamine.
Results:
results
showed
that
highest
(1300.3
μg
g−1
FW)
scopolamine
(918.06
accumulated
treated
L−1
CNP
at
48h
10
24h,
respectively.
present
effect
on
h6h
also
investigated
semi-quantitative
RT-PCR.
observed
at
concentration
24h
lowest
control
sample.
Conclusions:
Based
these
results,
it
concluded
CNP
can
be
an
effective
stimulant
for
production
plant
secondary
metabolites,
including
TA,
probably
due
stimulation
TA.
Язык: Английский
Cross-Kingdom Communication via Plant-Derived Extracellular Vesicle Nucleic Acids in Genetically Engineered Nicotiana tabacum
Genes,
Год журнала:
2025,
Номер
16(3), С. 356 - 356
Опубликована: Март 20, 2025
Background/Objectives:
Plants
release
extracellularly
lipid
bilayer-enclosed
vesicles
of
nanometric
size
that
can
be
retrieved
in
their
fluids.
Plant-derived
extracellular
(PDEVs)
have
mostly
been
involved
modulating
host–pathogen
interaction,
making
them
a
tool
for
cross-kingdom
communication
with
key
role
plant
immunity.
In
addition,
PDEVs
demonstrated
promising
therapeutic
features,
not
only
terms
intrinsic
nutraceutical
properties
but
also
active
molecules’
delivery.
Transgenic
plants
developed
variety
purposes,
i.e.,
to
improve
functional
like
crops,
produce
molecules.
However,
it
is
unclear
whether
transgenes
end
up
PDEVs,
thus
vehicle
diffusion
into
the
environment.
Methods:
Here,
we
investigated
association
transgenic
DNA
and
RNA
secreted
by
tobacco
(Nicotiana
tabacum)
engineered
express
neomycine
phosphotransferase
II
(Npt-II)
gene.
were
isolated
from
leaf
apoplastic
fluid
ultracentrifugation
characterized
morphology
size.
The
was
assessed
qRT-PCR
immunomodulatory
assaying
PDEVs-induced
IL1β
IL10
on
THP1
monocytes.
Results:
Npt-II
RNA,
DNA,
could
amplified
whereas
no
differences
observed
between
wt
properties.
Conclusions:
Although
different
behaviour
other
types
RNAs
or
DNAs
still
possible,
our
findings
indicate
this
model,
are
associated
they
protect
including
degradation,
contributing
spreading.
Язык: Английский
A roadmap to understanding and anticipating microbial gene transfer in soil communities
Microbiology and Molecular Biology Reviews,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 8, 2025
SUMMARY
Engineered
microbes
are
being
programmed
using
synthetic
DNA
for
applications
in
soil
to
overcome
global
challenges
related
climate
change,
energy,
food
security,
and
pollution.
However,
we
cannot
yet
predict
gene
transfer
processes
assess
the
frequency
of
unintentional
engineered
environmental
when
applying
biology
technologies
at
scale.
This
challenge
exists
because
complex
heterogeneous
characteristics
soils,
which
contribute
fitness
transport
cells
exchange
genetic
material
within
communities.
Here,
describe
knowledge
gaps
about
across
microbiomes.
We
propose
strategies
improve
our
understanding
communities,
highlight
need
benchmark
performance
biocontainment
measures
situ
,
discuss
responsibly
engaging
community
stakeholders.
opportunities
address
gaps,
such
as
creating
a
set
standards
studying
diverse
types
measuring
host
range
microbiomes
emerging
technologies.
By
comparing
rates,
range,
persistence
different
posit
that
community-scale,
environment-specific
models
can
be
built
anticipate
biotechnology
risks.
Such
studies
will
enable
design
safer
biotechnologies
allow
us
realize
benefits
mitigate
risks
associated
with
release
Язык: Английский
Genetic Insights and Molecular Breeding Approaches for Downy Mildew Resistance in Cucumber (Cucumis sativus L.): Current Progress and Future Prospects
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(23), С. 12726 - 12726
Опубликована: Ноя. 27, 2024
Cucurbit
downy
mildew,
caused
by
Pseudoperonospora
cubensis,
is
a
devastating
disease
in
cucumbers
that
leads
to
significant
yield
losses
many
cucurbit-growing
regions
worldwide.
Developing
resistant
cucumber
varieties
sustainable
approach
managing
this
disease,
especially
given
the
limitations
of
chemical
control
and
evolving
nature
pathogens.
This
article
reviews
genetic
basis
mildew
resistance
cucumbers,
emphasizing
key
(R)
genes
quantitative
trait
loci
(QTLs)
have
been
mapped.
Recent
advances
molecular
breeding
tools,
including
marker-assisted
selection
(MAS),
genomic
(GS),
CRISPR/Cas9
genome
editing,
accelerated
development
cultivars.
review
also
explores
role
transcriptomics,
genomics,
other
‘omics’
technologies
unraveling
mechanisms
behind
offers
insights
into
future
strategies
aimed
at
long-term
management.
Management
for
cucurbit
are
discussed,
along
with
potential
impacts
climate
change
on
occurrence
severity
highlighting
how
changing
environmental
conditions
may
influence
dynamics.
Integrating
these
advanced
approaches
traditional
promises
accelerate
mildew-resistant
varieties,
contributing
sustainability
resilience
production.
Язык: Английский