Phylogenomic and functional characterization of an evolutionary conserved cytochrome P450-based insecticide detoxification mechanism in bees

Proceedings of the National Academy of Sciences, Год журнала: 2022, Номер 119(26)

Опубликована: Июнь 21, 2022

The regulatory process for assessing the risks of pesticides to bees relies heavily on use honeybee, Apis mellifera , as a model other bee species. However, validity using A. surrogate and non- in pesticide risk assessment has been questioned. Related this line research, recent work shown that specific P450 enzymes belonging CYP9Q subfamily act critically important determinants insecticide sensitivity species by efficiently detoxifying certain chemotypes. extent which presence functional orthologs these is conserved across diversity unclear. Here we used phylogenomic approach identify > 100 putative 75 encompassing all major families. Functional analysis 26 P450s from 20 representative revealed P450-mediated detoxification systemic insecticides, including neonicotinoid thiacloprid butenolide flupyradifurone, pollinator our analyses also reveal -related genes are not universal species, with some Megachilidae lacking such genes. Thus, results an evolutionary capacity metabolize insecticides families while identifying small number where function may have lost. Furthermore, they illustrate potential toxicogenomic inform nonmanaged predicting capability break down synthetic insecticides.

Язык: Английский

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Divergent amplifications of CYP9A cytochrome P450 genes provide two noctuid pests with differential protection against xenobiotics

Proceedings of the National Academy of Sciences, Год журнала: 2023, Номер 120(37)

Опубликована: Сен. 5, 2023

Here, we provide mechanistic support for the involvement of CYP9A subfamily cytochrome P450 monooxygenases in detoxification host plant defense compounds and chemical insecticides Spodoptera exigua frugiperda . Our comparative genomics shows that a large cluster genes occurs two species but with significant differences its contents, including several species-specific duplicates substantial sequence divergence, both between orthologs duplicates. Bioassays CRISPR-Cas9 knockouts clusters show that, collectively, CYP9As can detoxify furanocoumarin (imperatorin xanthotoxin) representing three different chemotypes (pyrethroids, avermectins, oxadiazines). However, vitro metabolic assays heterologously expressed products individual particular activities against these compounds. We also find tight genetic linkage high levels pyrethroid resistance field strains species. propose their divergent amplifications have not only contributed to development broad ranges over long evolutionary timeframes supplied them diverse options evolving very recent past.

Язык: Английский

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Identification of differentially expressed miRNAs associated with diamide detoxification pathways in Spodoptera frugiperda

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Фев. 21, 2024

The fall armyworm (FAW) Spodoptera frugiperda is a severe economic pest of multiple crops globally. Control this often achieved using insecticides; however, over time, S. has developed resistance to new mode action compounds, including diamides. Previous studies have indicated diamide complex developmental process involving detoxification genes. Still, the mechanism underlying possible involvement microRNAs in post-transcriptional regulation not yet been elucidated. In study, global screen (miRNAs) revealed 109 known and 63 novel miRNAs. Nine miRNAs (four five novel) were differentially expressed between insecticide-resistant -susceptible strains. Gene Ontology analysis predicted putative target transcripts encoding significant genes belonging pathways. Additionally, are involved response exposure, indicating they probably associated with pathway. Thus, study provides comprehensive evidence for link repressed miRNA expression induced that possibly mediate through regulation. These findings highlight important clues further research unravel roles mechanisms conferring resistance.

Язык: Английский

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Differential detoxification enzyme profiles in C-corn strain and R-rice strain of Spodoptera frugiperda by comparative genomic analysis: insights into host adaptation

BMC Genomics, Год журнала: 2025, Номер 26(1)

Опубликована: Янв. 6, 2025

The fall armyworm (FAW) Spodoptera frugiperda, a highly invasive, polyphagous pest, poses global agricultural threat. It has two strains, the C-corn and R-rice each with distinct host preferences. This study compares detoxification enzyme gene families across these strains related species to explore their adaptation diverse plant metabolites. A total of 1,995 detoxification-related genes, including cytochrome P450 monooxygenases (CYPs), carboxylesterases (COEs), glutathione S-transferases (GSTs), UDP-glucuronosyltransferases (UGTs), ATP-binding cassette transporters (ABCs), were identified genus Spodoptera, S. littoralis, litura, picta, exigua, both FAW strains. higher abundance phase I enzymes (CYPs COEs) GSTs was observed in species, while exhibited fewer notable differences copy numbers between C R Analyses at subfamily level revealed significant variation distribution expression, particularly within II enzymes. Expansions CYP6AE detected strain, contractions GST-ε, CYP9A, CYP4M, UGT33B, UGT33F occurred In contrast, no substantial III ABC Functional predictions protein interaction networks suggest broader expansion metabolism-related genes strain compared strain. These findings emphasize pivotal role adaptation, providing molecular insights into FAW's capacity for range expansion, which are crucial devising targeted sustainable pest management strategies. differential expression different frugiperda.

Язык: Английский

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Inducible Gut-Specific Carboxylesterase SlCOE030 in Polyphagous Pests of Spodoptera litura Conferring Tolerance between Nicotine and Cyantraniliprole

Journal of Agricultural and Food Chemistry, Год журнала: 2023, Номер 71(10), С. 4281 - 4291

Опубликована: Март 6, 2023

Insecticides tolerance in herbivorous arthropods is associated with preadaptation to host plant allelochemicals. However, how secondary metabolites activate detoxifying metabolic genes develop remains unclear. Herein, the of Spodoptera litura larvae cyantraniliprole was increased after nicotine exposure. An S. α esterase, SlCOE030, predominantly expressed midgut and induced exposure cyantraniliprole, nicotine, plus nicotine. Drosophila melanogaster ectopically overexpressed SlCOE030 enhanced by 4.91- 2.12-fold, respectively. Compared UAS-SlCOE030 Esg-GAL4 lines, Esg > line laid more eggs knockdown decreased sensitivity nicotine-treated cyantraniliprole. Metabolism assays indicated that recombinant protein metabolizes Homology modeling molecular docking analysis demonstrated exhibits effective affinities for Thus, insect CarEs may result development cross-tolerance between synthetic insecticides metabolites.

Язык: Английский

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Identification of inducible CYP3 and CYP4 genes associated with abamectin tolerance in the fat body and Malpighian tubules of Spodoptera litura

Pesticide Biochemistry and Physiology, Год журнала: 2023, Номер 198, С. 105751 - 105751

Опубликована: Дек. 21, 2023

Язык: Английский

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Cytochrome P450s genes CYP321A9 and CYP9A58 contribute to host plant adaptation in the fall armyworm Spodoptera frugiperda

Pest Management Science, Год журнала: 2023, Номер 79(5), С. 1783 - 1790

Опубликована: Янв. 11, 2023

The fall armyworm, Spodoptera frugiperda is one of the most destructive agricultural pests, which can complete their entire life cycle on various plants. At present, some detoxification genes have been proved to be involved in adaptability plants insects. However, genetics behind insect pest responses host switches, and ability adapt new plants, remain poorly understood. This study was conducted evaluate adaptation S. plant determine roles CYP321A9 CYP9A58 metabolism armyworm.The results revealed that feeding maize more suitable for develop compared with rice. In addition, knocking down SfCYP321A9 SfCYP9A58 resulted a prolonged developmental time larvae fed Meanwhile, RNAi knockdown significantly higher mortality when exposed rice allelochemicals, ferulic acid, gramine tricin. Furthermore, overexpression reduced Drosophila melanogaster tricin.Our suggest play key role frugiperda, contribute greater understanding molecular basis provide means effective management tools resistance. © 2023 Society Chemical Industry.

Язык: Английский

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UDP-Glycosyltransferases UGT350C3 and UGT344L7 Confer Tolerance to Neonicotinoids in Field Populations of Aphis gossypii

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(25), С. 14141 - 14151

Опубликована: Июнь 12, 2024

The cotton aphid,

Язык: Английский

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Rapid Adaptation and Interspecific Introgression in the North American Crop Pest Helicoverpa zea

Molecular Biology and Evolution, Год журнала: 2024, Номер 41(7)

Опубликована: Июнь 27, 2024

Insect crop pests threaten global food security. This threat is amplified through the spread of nonnative species and adaptation native to control measures. Adaptations such as pesticide resistance can result from selection on variation within a population, or gene flow another population. We investigate these processes in an economically important noctuid pest, Helicoverpa zea, which has evolved wide range pesticides. Its sister armigera, first detected invasive Brazil 2013, introduced pyrethroid-resistance CYP337B3 South American H. zea via adaptive introgression. To understand whether this could contribute North America, we sequenced 237 genomes across 10 sample sites. report armigera introgression into Two individuals sampled Texas 2019 carry haplotypes 4 Mbp region containing CYP337B3. Next, identify signatures panmictic population nonadmixed identifying selective sweep at second cytochrome P450 gene: CYP333B3. estimate that its derived allele conferred ∼5% fitness advantage show explains independently observed rare nonsynonymous CYP333B3 mutations approaching fixation over ∼20-year period. also detect putative kinesin associated with Bt resistance. Overall, document two mechanisms rapid adaptation: introduction fitness-enhancing alleles interspecific introgression, intraspecific variation.

Язык: Английский

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Functionally characterized arthropod pest and pollinator cytochrome P450s associated with xenobiotic metabolism

Pesticide Biochemistry and Physiology, Год журнала: 2021, Номер 181, С. 105005 - 105005

Опубликована: Дек. 13, 2021

Язык: Английский

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