Genetic characterisation of candidate ecdysteroid kinases inDrosophila melanogaster DOI Creative Commons
Jack L. Scanlan, Charles Robin

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 25, 2024

Abstract Ecdysteroids are major hormones in insects and control moulting, growth, reproduction, physiology, behaviour. The biosynthesis of ecdysteroids such as 20-hydroxyecdysone (20E) from dietary sterols is well characterised, but ecdysteroid catabolism poorly understood. Ecdysteroid kinases (EcKs) mediate the reversible phosphorylation ecdysteroids, which has been implicated recycling during embryogenesis reproduction various insects. However, to date only two EcK-encoding genes have identified, silkworm Bombyx mori mosquito Anopheles gambiae . Previously, we identified kinase-like (EcKL) genes— Wallflower ( Wall ) Pinkman pkm )—in model fruit fly Drosophila melanogaster that orthologs 22-kinase gene BmEc22K Here, using knockdown, knockout misexpression, explore ’s possible functions genetically test hypothesis they encode EcKs. null mutants viable fertile, suggesting not essential for development or whereas phenotypes arising RNAi somatic CRISPR appear derive off-target effects other artefacts. misexpression results dramatic phenotypes, including developmental arrest, defects trachea, cuticle pigmentation. fails phenocopy irreversible through Cyp18a1 , does directly inactivate 20E. Additionally, attenuated mutants, strongly an 26-kinase. We hypothesise substrate this experiment possibly generally unknown, atypical plays roles development, may highlight aspects insect endocrinology as-yet uncharacterised. also provide preliminary evidence CG5644 encodes conserved across Diptera.

Язык: Английский

Expression of short neuropeptide F and prothoracicotropic hormone in relation to photoperiodic regulation of pupariation and pupal diapause in the flesh fly, Sarcophaga similis (Diptera: Sarcophagidae) DOI

N Yoshinaga,

Shin G. Goto

Applied Entomology and Zoology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 22, 2025

Язык: Английский

Процитировано

0
Insect nuclear receptors: From orphans to ligands DOI
Wen Liu, Hang Chen, Kirst King‐Jones

и другие.

Elsevier eBooks, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Cis-regulatory polymorphism at fiz ecdysone oxidase contributes to polygenic evolutionary response to malnutrition in Drosophila DOI Creative Commons
Fanny Cavigliasso, Mikhail Savitsky, Alexey Koval

и другие.

PLoS Genetics, Год журнала: 2024, Номер 20(3), С. e1011204 - e1011204

Опубликована: Март 7, 2024

We investigate the contribution of a candidate gene, fiz ( fezzik ), to complex polygenic adaptation juvenile malnutrition in Drosophila melanogaster . Experimental populations maintained for >250 generations experimental evolution nutritionally poor larval diet (Selected populations) evolved several-fold lower expression compared unselected Control populations. Here we show that this divergence is mediated by cis -regulatory polymorphism. This polymorphism, originally sampled from natural population Switzerland, distinct second SNP previously identified non-African D populations, implying two independent variants promoting high segregate Enzymatic analyses Fiz protein expressed E coli demonstrate it has ecdysone oxidase activity acting on both and 20-hydroxyecdysone. Four five paralogs annotated ecdysteroid metabolism also reduced Selected larvae, malnutrition-driven selection favored general downregulation oxidases. Finally, as an test role adaptation, knockdown RNAi results faster growth diet, but at cost greatly survival. These imply was because its suppressing response nutrient shortage. However, they suggest only adaptive combination with other changes which ensure organism can sustain promoted downregulation.

Язык: Английский

Процитировано

3
Evolution of insect metamorphosis – an update DOI
Barbora Konopová

Current Opinion in Insect Science, Год журнала: 2024, Номер 67, С. 101289 - 101289

Опубликована: Окт. 30, 2024

Язык: Английский

Процитировано

3
Eclosion muscles secrete ecdysteroids to initiate asymmetric intestinal stem cell division in Drosophila DOI Creative Commons
Song Zhang, Song Wu,

Ruining Yao

и другие.

Developmental Cell, Год журнала: 2023, Номер 59(1), С. 125 - 140.e12

Опубликована: Дек. 13, 2023

Язык: Английский

Процитировано

6
KDM5-mediated activation of genes required for mitochondrial biology is necessary for viability in Drosophila DOI Creative Commons
Michael F. Rogers, Owen J. Marshall, Julie Secombe

и другие.

Development, Год журнала: 2023, Номер 150(21)

Опубликована: Окт. 6, 2023

ABSTRACT Histone-modifying proteins play important roles in the precise regulation of transcriptional programs that coordinate development. KDM5 family interact with chromatin through demethylation H3K4me3 as well demethylase-independent mechanisms remain less understood. To gain fundamental insights into activities proteins, we examined essential single Drosophila Kdm5 ortholog during performs crucial functions larval neuroendocrine prothoracic gland, providing a model to study its role regulating key gene expression programs. Integrating genome binding and transcriptomic data, identify regulates genes required for function maintenance mitochondria, find loss causes morphological changes mitochondria. This is developmental KDM5, mitochondrial biogenesis transcription factor Ets97D, homolog GABPα, able suppress altered morphology lethality null animals. Together, these data establish KDM5-mediated cellular are normal development could contribute KDM5-linked disorders when dysregulated.

Язык: Английский

Процитировано

4
Genetic characterization of candidate ecdysteroid kinases in Drosophila melanogaster DOI Creative Commons
Jack L. Scanlan, Charles Robin

G3 Genes Genomes Genetics, Год журнала: 2024, Номер 14(11)

Опубликована: Авг. 29, 2024

Ecdysteroids are major hormones in insects and control molting, growth, reproduction, physiology, behavior. The biosynthesis of ecdysteroids such as 20-hydroxyecdysone (20E) from dietary sterols is well characterized, but ecdysteroid catabolism poorly understood. Ecdysteroid kinases (EcKs) mediate the reversible phosphorylation ecdysteroids, which has been implicated recycling during embryogenesis reproduction various insects. However, to date, only 2 EcK-encoding genes have identified, silkworm Bombyx mori mosquito Anopheles gambiae. Previously, we identified kinase-like (EcKL) genes-Wallflower (Wall) Pinkman (pkm)-in model fruit fly Drosophila melanogaster that orthologs 22-kinase gene BmEc22K. Here, using knockdown, knockout, misexpression, explore Wall pkm's possible functions genetically test hypothesis they encode EcKs. pkm null mutants viable fertile, suggesting not essential for development or whereas phenotypes arising RNAi somatic CRISPR appear derive off-target effects other artifacts. misexpression results dramatic phenotypes, including developmental arrest, defects trachea, cuticle, pigmentation. fails phenocopy irreversible through Cyp18a1, does directly inactivate 20E. Additionally, attenuated Cyp18a1 mutants, strongly an 26-kinase. We hypothesize substrate this experiment possibly generally unknown, atypical plays roles development, may highlight aspects insect endocrinology as-yet uncharacterized. also provide preliminary evidence CG5644 encodes conserved across Diptera.

Язык: Английский

Процитировано

1
Ptth regulates lifespan through innate immunity pathway inDrosophila DOI Creative Commons
Ping Kang, Peiduo Liu, Jin Oh Kim

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Окт. 2, 2023

The prothoracicotropic hormone (PTTH) is a well-known neuropeptide that regulates insect metamorphosis (the juvenile-to-adult transition) by inducing the biosynthesis of steroid hormones. However, role PTTH in adult physiology and longevity largely unexplored. Here, we show Ptth loss-of-function mutants are long-lived exhibit increased resistance to oxidative stress Drosophila. Intriguingly, find loss blunt age-dependent upregulation NF-κB signaling specifically fly hepatocytes (oenocytes). We further oenocyte-specific overexpression Relish/NF-κB blocks lifespan extension mutants, suggesting through signaling. Surprisingly, adult-specific knockdown did not prolong lifespan, indicating controls developmental programs. Indeed, receptor Torso prothoracic gland (PG) during development prolongs lifespan. To uncover processes underlying PTTH-regulated perform transcriptomic analysis identify an unexpected activation developing oenocytes metamorphosis, which blocked mutants. Importantly, early pupal stages significantly flies. Thus, our findings controlling temporal spatial highlight vital shaping physiology.

Язык: Английский

Процитировано

3
Cis-regulatory polymorphism atfizecdysone oxidase contributes to polygenic adaptation to malnutrition inDrosophila DOI Creative Commons
Fanny Cavigliasso,

Mikhail Savitskiy,

Alexey Koval

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Авг. 29, 2023

Abstract We investigate the contribution of a candidate gene, fiz ( fezzik ), to complex polygenic adaptation juvenile malnutrition in Drosophila melanogaster . show that experimental populations adapted during >250 generations evolution nutritionally poor larval diet (Selected populations) evolved several-fold lower expression compared unselected Control populations. This divergence is mediated by cis-regulatory polymorphism. polymorphism, which was originally present sample from natural population Switzerland, distinct second SNP previously identified non-African D. populations, implying two independent variants promoting high segregate Enzymatic analyses Fiz protein expressed E. coli demonstrate it has ecdysone oxidase activity acting on both and 20-hydroxyecdysone. Four five paralogs annotated ecdysteroid metabolism also reduced Selected larvae, suggesting malnutrition-driven selection favored general downregulation oxidases. Finally, as an test role adaptation, we knockdown RNAi results faster growth diet, but at cost greatly survival. These imply because its suppressing response nutrient shortage. However, only adaptive combination with other changes such acquisition metabolism, ensure organism can actually sustain promoted downregulation.

Язык: Английский

Процитировано

2
Genetic characterisation of candidate ecdysteroid kinases inDrosophila melanogaster DOI Creative Commons
Jack L. Scanlan, Charles Robin

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 25, 2024

Abstract Ecdysteroids are major hormones in insects and control moulting, growth, reproduction, physiology, behaviour. The biosynthesis of ecdysteroids such as 20-hydroxyecdysone (20E) from dietary sterols is well characterised, but ecdysteroid catabolism poorly understood. Ecdysteroid kinases (EcKs) mediate the reversible phosphorylation ecdysteroids, which has been implicated recycling during embryogenesis reproduction various insects. However, to date only two EcK-encoding genes have identified, silkworm Bombyx mori mosquito Anopheles gambiae . Previously, we identified kinase-like (EcKL) genes— Wallflower ( Wall ) Pinkman pkm )—in model fruit fly Drosophila melanogaster that orthologs 22-kinase gene BmEc22K Here, using knockdown, knockout misexpression, explore ’s possible functions genetically test hypothesis they encode EcKs. null mutants viable fertile, suggesting not essential for development or whereas phenotypes arising RNAi somatic CRISPR appear derive off-target effects other artefacts. misexpression results dramatic phenotypes, including developmental arrest, defects trachea, cuticle pigmentation. fails phenocopy irreversible through Cyp18a1 , does directly inactivate 20E. Additionally, attenuated mutants, strongly an 26-kinase. We hypothesise substrate this experiment possibly generally unknown, atypical plays roles development, may highlight aspects insect endocrinology as-yet uncharacterised. also provide preliminary evidence CG5644 encodes conserved across Diptera.

Язык: Английский

Процитировано

0