Sex determination in moths and butterflies: Masculinizer as key player
Current Opinion in Insect Science,
Год журнала:
2025,
Номер
unknown, С. 101375 - 101375
Опубликована: Апрель 1, 2025
Язык: Английский
Masculinizer gene controls male sex determination in the codling moth, Cydia pomonella
Insect Biochemistry and Molecular Biology,
Год журнала:
2023,
Номер
160, С. 103991 - 103991
Опубликована: Авг. 1, 2023
Язык: Английский
Dichotomous sperm in Lepidopteran insects: a biorational target for pest management
Frontiers in Insect Science,
Год журнала:
2023,
Номер
3
Опубликована: Авг. 23, 2023
Lepidoptera
are
unusual
in
possessing
two
distinct
kinds
of
sperm,
regular
nucleated
(eupyrene)
sperm
and
anucleate
(apyrene)
(‘parasperm’).
Sperm
both
types
transferred
to
the
female
required
for
male
fertility.
Apyrene
play
‘helper’
roles,
assisting
eupyrene
gain
access
unfertilized
eggs
influencing
reproductive
behavior
mated
moths.
development
promising
targets
environmentally
safer,
target-specific
biorational
control
strategies
lepidopteran
pest
insects.
dimorphism
provides
a
wide
window
which
manipulate
functionality
dynamics,
thereby
impairing
fitness
species.
Opportunities
interfere
with
spermatozoa
available
not
only
while
still
(before
copulation),
but
also
(after
copulation,
when
male-provided
spermatophore,
or
during
storage
female’s
spermatheca).
Biomolecular
technologies
like
RNAi,
miRNAs
CRISPR-Cas9
achieve
by
targeting
genes
directly
indirectly
involved
dichotomous
production,
function,
persistence.
Язык: Английский
Dosage compensation in non-model insects – progress and perspectives
Trends in Genetics,
Год журнала:
2024,
Номер
41(1), С. 76 - 98
Опубликована: Сен. 27, 2024
Язык: Английский
A W chromosome-derived feminizing piRNA in pyralid moths demonstrates convergent evolution for primary sex determination signals in Lepidoptera
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 26, 2024
Abstract
Background
The
primary
signals
of
sex
determination
in
insects
are
diverse
and
evolve
erratically.
This
also
appears
to
be
the
case
with
moths
butterflies.
In
silkworm
Bombyx
mori,
female
development
is
triggered
by
a
W-linked
Feminizer
(
Fem)
piRNA
degrading
mRNA
Z-linked
Masculinizer
(
Masc)
gene,
which
controls
male
development.
However,
Fem
absent
related
Trilocha
varians.
Since
mechanism
similar
that
has
evolved
phylogenetically
distant
Plutella
xylostella,
we
investigated
whether
this
conserved
other
groups
Lepidoptera.
Results
We
examined
W
chromosome
Ephestia
kuehniella
for
non-coding
feminizing
genes
identified
putative
many
partial
copies
EkMasc
gene.
generated
repetitive
sequence
named
E.
kuehniella
Moth-overruler-of-masculinization
(
EkMom).
EkMom
shows
high
similarity
region
expressed
at
onset
development,
suggesting
it
likely
trigger
determination.
then
mapped
small
RNA-seq
data
from
embryos
Plodia
interpunctella
PiMasc
gene
single
RNA,
PiMom
piRNA,
able
target
PiMasc,
identity
piRNA.
Both
repeats
present
copy
number
form
cluster
on
chromosome.
Conclusions
Our
study
provides
multiple
lines
evidence
sex-determining
signal
two
pyralid
highlights
possible
pathway
origin
piRNAs
A
between
proposed
P.
(Pyralidae)
xylostella
(Plutellidae)
function
B.
mori
(Bombycidae)
suggests
convergent
evolution
Язык: Английский
Masculinizer Gene Controls Male Sex Determination in the Codling Moth, Cydia Pomonella
Опубликована: Янв. 1, 2023
The
molecular
mechanisms
of
sex
determination
in
moths
and
butterflies
(Lepidoptera)
with
female
heterogamety
(WZ/ZZ)
are
poorly
understood,
except
the
silkworm
Bombyx
mori.
However,
Masculinizer
(Masc)
gene
that
controls
male
development
dosage
compensation
B.
mori,
appears
to
be
conserved
Lepidoptera,
as
its
masculinizing
function
was
recently
confirmed
several
moth
species.
In
this
work,
we
investigated
role
Masc
codling
Cydia
pomonella
(Tortricidae),
a
globally
important
pest
pome
fruits
walnuts.
structure
C.
ortholog,
CpMasc,
is
similar
mori
Masc.
unlike
identified
14
splice
variants
CpMasc
available
transcriptomes.
Subsequent
screening
for
specificity
genetic
variation
using
publicly
data
RT-PCR
revealed
three
male-specific
variants.
Then
qPCR
analysis
these
sex-specific
expression
showing
peak
only
early
embryos.
Knockdown
by
RNAi
during
embryogenesis
resulted
shift
from
male-
female-specific
splicing
doublesex
(Cpdsx)
gene,
downstream
effector,
ZZ
embryos,
leading
strongly
female-biased
ratio.
These
clearly
demonstrate
functions
sex-determining
cascade
pomonella.
Our
study
also
showed
transcripts
provided
maternally,
they
were
detected
unfertilized
eggs
after
oviposition
mature
dissected
virgin
females.
This
finding
unique,
maternal
provision
mRNA
has
rarely
been
studied
Lepidoptera.
Язык: Английский
Non-coding RNAs in Lepidoptera
IntechOpen eBooks,
Год журнала:
2023,
Номер
unknown
Опубликована: Сен. 24, 2023
In
the
last
few
years,
amount
of
genomic
sequence
data
has
grown
exponentially.
A
large
number
non-coding
RNAs
(ncRNAs)
have
been
identified
from
bacteria
to
humans.
ncRNAs
are
various
and
multi-faced;
they
can
regulate
gene
expression
through
chromosomal,
transcriptional,
post-transcriptional,
translational
levels
then
participate
in
whole
process
development
different
organisms.
1980s
Lepidoptera;
play
roles
growth,
metamorphosis,
metabolism,
sex
determination,
reproduction,
immune
response
insects.
Now,
use
pest
control
Lepidoptera
is
also
process.
This
chapter
will
review
recent
advance
prospect
future
studies
Язык: Английский