Biochemical and Biophysical Research Communications, Год журнала: 2024, Номер 726, С. 150276 - 150276
Опубликована: Июнь 18, 2024
Язык: Английский
Developmental Cell, Год журнала: 2023, Номер 59(1), С. 125 - 140.e12
Опубликована: Дек. 13, 2023
Язык: Английский
Процитировано
6
Insect Molecular Biology, Год журнала: 2024, Номер 33(6), С. 792 - 805
Опубликована: Июль 15, 2024
Abstract Metamorphosis plays an important role in the evolutionary success of insects. Accumulating evidence indicated that microRNAs (miRNAs) are involved regulation processes associated with insect metamorphosis. However, miRNAs coordinated juvenile hormone (JH)‐regulated metamorphosis remain poorly reported. In present study, using high‐throughput miRNA sequencing combined Drosophila genetic approaches, we demonstrated miR‐iab‐8, which primarily targets homeotic genes to modulate haltere‐wing transformation and sterility was up‐regulated by JH JH‐mediated Overexpression miR‐iab‐8 fat body resulted delayed development failure larval‐pupal transition. Furthermore, metabolomic analysis results revealed overexpression caused severe energy metabolism defects especially lipid metabolism, resulting significantly reduced triacylglycerol (TG) content glycerophospholipids but enhanced accumulation phosphatidylcholine (PC) phosphatidylethanolamine (PE). line this, Nile red staining during third larval development, TG larvae continuously decreased, is opposite control. Additionally, transcription levels committed synthesis breakdown were found be increased expression responsible for also altered. Overall, proposed induced perturb homeostasis storage body, turn affected growth
Язык: Английский
Процитировано
1
Insect Molecular Biology, Год журнала: 2023, Номер 33(2), С. 124 - 135
Опубликована: Ноя. 2, 2023
Abstract Differentiation of imaginal epidermal cells Drosophila melanogaster to form adult cuticles occurs at approximately 40–93 h after puparium formation. Juvenile hormone (JH) given pupariation results in formation a second pupal cuticle the abdomen instead cuticle. Although gene Acp65A has been reported be down‐regulated following JH treatment, regulatory mechanism remains unclear. Here, we found that primary response Krüppel homologue 1 ( Kr‐h1 ) plays vital role repression through mediation action. Overexpression mimicked—while knocking down attenuated—the inhibitory action on abdominal Further, inhibited transcription by directly binding consensus site (KBS) within promoter region. Moreover, DNA methyltransferase Dnmt2 was shown interact with Kr‐h1, combined KBS promote methylation sequences around KBS, turn inhibiting . This study advances our understanding molecular basis “status quo” metamorphosis.
Язык: Английский
Процитировано
1
Biochemical and Biophysical Research Communications, Год журнала: 2024, Номер 726, С. 150276 - 150276
Опубликована: Июнь 18, 2024
Язык: Английский
Процитировано
0